Email Alerts
RSS
Volume 39 Issue 3
Mar.  2023
Turn off MathJax
Article Contents
Article Navigation >  CHINESE JOURNAL OF BURNS AND WOUNDS  > 2023  >  39(3): 256-263
Zheng F,Cai YE,Li L,et al.Effects and mechanism of astragalus polysaccharide on wound healing of deep partial-thickness burns in rats[J].Chin J Burns Wounds,2023,39(3):256-263.DOI: 10.3760/cma.j.cn501225-20220324-00087.
Citation: Zheng F,Cai YE,Li L,et al.Effects and mechanism of astragalus polysaccharide on wound healing of deep partial-thickness burns in rats[J].Chin J Burns Wounds,2023,39(3):256-263.DOI: 10.3760/cma.j.cn501225-20220324-00087.
shu

Effects and mechanism of astragalus polysaccharide on wound healing of deep partial-thickness burns in rats

doi: 10.3760/cma.j.cn501225-20220324-00087
  • 1.

    Department of Burns and Plastic Surgery, the First People's Hospital of Shangqiu, Shangqiu 476100, China

  • 2.

    Department of Burns, the First People's Hospital of Zhengzhou, Zhengzhou 450004, China

Funds:

Joint Construction Project of Henan Medical Science and Technology Project LHGJ20191002

More Information
  • Corresponding author: Zheng Fan, Email: 358517964@qq.com
  • Received Date: 2022-03-24
    Abstract
  •   Objective   To investigate the effects and mechanism of astragalus polysaccharide (APS) on wound healing of deep partial-thickness burns in rats.   Methods   The experimental study method was used. Fifty 7-week-old male Sprague-Dawley rats were divided into normal group, simple burn group, APS group, inhibitor group, and inhibitor+APS group according to the random number table, with 10 rats in each group. Except for normal group, rats in the other 4 groups were inflicted with a deep partial-thickness burn wound on the back. Rats in normal group and simple burn group were intraperitoneally injected with normal saline, and rats in the other three groups were injected with APS and/or integrin-linked kinase (ILK) inhibitor, respectively. After 7 days of injection, the wound healing rate of rats with burns in the four groups was calculated, and the serum content of interferon-γ, interleukin-2 (IL-2), and tumor necrosis factor α (TNF-α) in rats in 5 groups was determined by enzyme-linked immunosorbent assay (ELISA). The normal skin tissue of rats in normal group and wound tissue of rats with burns in the four groups were taken, the water content was determined and the water ratio was calculated, the content of interferon-γ, IL-2, and TNF-α was detected by ELISA, the mRNA expressions of epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), and ILK were detected by real-time fluorescence quantitative reverse transcription polymerase chain reaction, and the protein expressions of ILK, protein kinase B (Akt), phosphorylated Akt (p-Akt), glycogen synthetic kinase-3β (GSK-3β), and phosphorylated GSK-3β (p-GSK-3β) were detected by Western blotting. Data were statistically analyzed with one-way analysis of variance and least significant difference test.   Results   After 7 days of injection, the wound healing rate of rats in APS group was (67±5)%, which was significantly higher than (52±4)% in simple burn group and (59±5)% in inhibitor+APS group (with all the P values <0.05). The wound healing rate of rats in inhibitor+APS group was significantly higher than (48±4)% in inhibitor group ( P<0.05). After 7 days of injection, compared with those in serum or normal skin tissue of rats in normal group, the serum content of interferon-γ, TNF-α, IL-2 and the water ratio of wound tissue of rats in simple burn group were significantly increased ( P<0.05); compared with those in APS group, the serum content of interferon-γ, TNF-α, IL-2 and the water ratio of wound tissue of rats in simple burn group and inhibitor+APS group were significantly increased ( P<0.05); compared with those in inhibitor group, the serum content of interferon-γ, TNF-α, IL-2 and the water ratio of wound tissue of rats in inhibitor+APS group were significantly decreased ( P<0.05). After 7 days of injection, compared with that in normal skin tissue of rats in normal group, the content of interferon-γ, TNF-α, and IL-2 in wound tissue of rats in simple burn group was significantly increased ( P<0.05); compared with that in APS group, the content of interferon-γ, TNF-α, and IL-2 in wound tissue of rats in simple burn group and inhibitor+APS group was significantly increased ( P<0.05); compared with that in inhibitor group, the content of interferon-γ, TNF-α, and IL-2 in wound tissue of rats in inhibitor+APS group was significantly decreased ( P<0.05). After 7 days of injection, compared with those in normal skin tissue of rats in normal group, the mRNA expressions of EGF, bFGF, ILK and protein expressions of ILK, p-Akt, p-GSK-3β in wound tissue of rats in simple burn group were significantly increased ( P<0.05); compared with those in APS group, the mRNA expressions of EGF, bFGF, ILK and protein expressions of ILK, p-Akt, p-GSK-3β in wound tissue of rats in simple burn group and inhibitor+APS group were significantly decreased ( P<0.05); compared with those in inhibitor group, the mRNA expressions of EGF, bFGF, ILK and protein expressions of ILK, p-Akt, p-GSK-3β in wound tissue of rats in inhibitor+APS group were significantly increased ( P<0.05). There were no statistically significant differences in the protein expressions of Akt and GSK-3β in normal skin tissue of rats in normal group and wound tissue of rats with burns in the four groups ( P>0.05).   Conclusions   APS can alleviate systemic and local inflammation, alleviate tissue edema, and promote the expressions of healing factors in rats with deep partial-thickness burns, thus to promote the wound healing, possibly by activating ILK/Akt/GSK-3β signaling pathway.

     

    • FullText(HTML)
  • loading
    • References(30)
  • [1]
    HeM,XieWQ,ChengG,et al.The therapeutic effects of earthworm extract on deep second-degree burn wound healing[J].Ann Palliat Med,2021,10(3):2869-2879.DOI: 10.21037/apm-20-2393.
    [2]
    王子恩,王顺宾,陈昭宏.不同剂量多巴胺对严重烫伤大鼠早期脏器功能的影响[J].中华烧伤杂志,2019,35(3):179-185.DOI: 10.3760/cma.j.issn.1009-2587.2019.03.004.
    [3]
    白海良,段红杰,陈晨,等.Janus激酶/信号转导及转录激活子3通路抑制剂对严重烧伤大鼠骨骼肌功能的影响及其机制[J].中华烧伤杂志,2021,37(3):271-278.DOI: 10.3760/cma.j.cn501120-20200120-00030.
    [4]
    ZhangF, QiuXC, WangJJ, et al. Burn-related dysregulation of inflammation and immunity in experimental and clinical studies[J]. J Burn Care Res, 2017,38(6):e892-e899. DOI: 10.1097/BCR.0000000000000511.
    [5]
    XuX,CheL,XuL,et al.Green preparation of anti-inflammation an injectable 3D porous hydrogel for speeding up deep second-degree scald wound healing[J].RSC Adv,2020,10(59):36101-36110.DOI: 10.1039/d0ra04990e.
    [6]
    WangY,BeekmanJ,HewJ,et al.Burn injury: challenges and advances in burn wound healing, infection, pain and scarring[J].Adv Drug Deliv Rev,2018,123:3-17.DOI: 10.1016/j.addr.2017.09.018.
    [7]
    郑健生,刘胜利,彭晓菁,等.自体富血小板血浆联合Meek微型皮片修复严重烧伤患者四肢创面的效果及其机制的前瞻性研究[J].中华烧伤杂志,2021,37(8):731-737.DOI: 10.3760/cma.j.cn501120-20200427-00241.
    [8]
    何佳,鄢波,宋晓征,等.黄芪多糖缓解急性脑缺血再灌注损伤的氧化应激反应和免疫功能紊乱[J].中国免疫学杂志,2019,35(12):1443-1447,1452.DOI: 10.3969/j.issn.1000-484X.2019.12.007.
    [9]
    孙奇林,鞠婧,王浩,等.黄芪多糖干预糖尿病心肌氧化应激的实验研究[J].中国中西医结合杂志,2020,40(2):196-203.DOI: 10.7661/j.cjim.20191101.250.
    [10]
    李莉. 黄芪多糖对深Ⅱ度烧伤大鼠免疫功能及创面巨噬细胞炎症蛋白-2和单核细胞趋化蛋白-1表达的影响[J]. 中国临床药理学杂志, 2020, 36(9):1110-1113. DOI: 10.13699/j.cnki.1001-6821.2020.09.018.
    [11]
    巩文艺,韩冬.黄芪多糖对严重烧伤大鼠心肌组织氧化应激和炎症反应的影响[J].中国中医急症,2016,25(6):1005-1007,1022.DOI: 10.3969/j.issn.1004-745X.2016.06.017.
    [12]
    姜海,刘宇,徐刚.整合素连接激酶在大鼠烧伤创面中的表达及对创面愈合的影响[J].中国老年学杂志,2018,38(10):2462-2464.DOI: 10.3969/j.issn.1005-9202.2018.10.063.
    [13]
    李相国,杜万雪,徐娅丽,等.基于ILK信号通路探究马桑水提取物影响大鼠烧伤创面愈合和瘢痕增生的机制[J].中国病理生理杂志,2020,36(2):336-345.
    [14]
    周日兴,李叶扬,李罡,等.整合素连接激酶信号通路在糖尿病大鼠皮肤病变及创面愈合中的作用[J].中华烧伤杂志,2016,32(4):216-223.DOI: 10.3760/cma.j.issn.1009-2587.2016.04.006.
    [15]
    王士源,袁光海,鲁尧,等.负荷缓释VEGF的人工真皮对大鼠深Ⅱ度烧伤创面修复及细胞凋亡的影响[J].临床和实验医学杂志,2021,20(21):2259-2262.DOI: 10.3969/j.issn.1671-4695.2021.21.006.
    [16]
    YanY,WuR,BoY,et al.Induced pluripotent stem cells-derived microvesicles accelerate deep second-degree burn wound healing in mice through miR-16-5p-mediated promotion of keratinocytes migration[J].Theranostics,2020,10(22):9970-9983.DOI: 10.7150/thno.46639.
    [17]
    ZhaoB,ZhangX,HanW,et al.Wound healing effect of an astragalus membranaceus polysaccharide and its mechanism[J].Mol Med Rep,2017,15(6):4077-4083.DOI: 10.3892/mmr.2017.6488.
    [18]
    张臻,阙华发.黄芪多糖与糖尿病创面愈合相关机制研究进展[J].中医学报,2020,35(6):1202-1206.DOI: 10.16368/j.issn.1674-8999.2020.06.270.
    [19]
    WuR,DuD,BoY,et al.Hsp90α promotes the migration of iPSCs-derived keratinocyte to accelerate deep second-degree burn wound healing in mice[J].Biochem Biophys Res Commun,2019,520(1):145-151.DOI: 10.1016/j.bbrc.2019.09.120.
    [20]
    于泽洋,李天博,王江宁.金鸡毛草提取物经Wnt/β-catenin信号通路促进大鼠Ⅲ期压疮溃疡愈合的作用研究[J].中国中医急症,2021,30(4):612-616.DOI: 10.3969/j.issn.1004-745X.2021.04.012.
    [21]
    LiS,YangP,DingX,et al.Puerarin improves diabetic wound healing via regulation of macrophage M2 polarization phenotype[J/OL].Burns Trauma,2022,10:tkac046[2023-02-20].https://pubmed.ncbi.nlm.nih.gov/36568527/.DOI: 10.1093/burnst/tkac046.
    [22]
    TsaiKH,ShiH,ParungaoRJ,et al.Skin 11β-hydroxysteroid dehydrogenase type 1 enzyme expression regulates burn wound healing and can be targeted to modify scar characteristics[J/OL].Burns Trauma,2023,11:tkac052[2023-02-23].https://pubmed.ncbi.nlm.nih.gov/36694861/.DOI: 10.1093/burnst/tkac052.
    [23]
    BiglariS,LeTL,TanRP,et al.Simulating inflammation in a wound microenvironment using a dermal wound-on-a-chip model[J].Adv Healthc Mater,2019,8(1):e1801307.DOI: 10.1002/adhm.201801307.
    [24]
    刘川玉,唐建红,何洁,等.芦荟凝胶对深Ⅱ度烫伤大鼠创面愈合及EGF、bFGF表达的影响[J].中成药,2019,41(1):201-203.DOI: 10.3969/j.issn.1001-1528.2019.01.041.
    [25]
    SeoY,HeoY,JoS,et al.Novel positive allosteric modulator of protease-activated receptor 1 promotes skin wound healing in hairless mice[J].Br J Pharmacol,2021,178(17):3414-3427.DOI: 10.1111/bph.15489.
    [26]
    姜海,刘宇,徐刚.整合素连接激酶在大鼠烧伤创面中的表达及对创面愈合的影响[J].中国老年学杂志,2018,38(10):2462-2464.DOI: 10.3969/j.issn.1005-9202.2018.10.063.
    [27]
    王晓红,李叶扬,李罡,等.特异性整合素连接激酶抑制剂QLT0267对大鼠烧伤创面愈合影响的初步研究[J/CD].中华损伤与修复杂志:电子版,2014,9(3):20-23. DOI: 10.3877/cma.j.issn.1673-9450.2014.03.007.
    [28]
    牛欢,陈曼丽,董博,等.Urocortin-I通过激活Akt/GSK-3β改善I/R心肌单相动作电位及氧化炎症反应[J].中国动脉硬化杂志,2020,28(1):31-36.DOI: 10.3969/j.issn.1007-3949.2020.01.007.
    [29]
    FengY, LeF, TianP, et al. GTW inhibits the epithelial to mesenchymal transition of epithelial ovarian cancer via ILK/Akt/GSK3β/Slug signaling pathway[J]. J Cancer, 2021, 12(5):1386-1397. DOI: 10.7150/jca.52418.
    [30]
    ZhangS, ChenC, YingJ, et al. Alda-1, an aldehyde dehydrogenase 2 agonist, improves cutaneous wound healing by activating epidermal keratinocytes via Akt/GSK-3β/β-catenin pathway[J]. Aesthetic Plast Surg, 2020, 44(3):993-1005. DOI: 10.1007/s00266-020-01614-4.
    • Relative Articles
    • Supplements(0)
    • Cited By
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(3)  / Tables(3)

    Get Citation
    PDF
    XML

    Article Metrics

    Article views (1248) PDF downloads(33) Cited by()
    Proportional views
    Related

    Copyright © Chinese Journal of Burns京ICP备07035254号-14

    E-mail：shaoshangzazhi@163.com

    Website：https://zhsszz.xml-journal.net/

    Supported by: Beijing Renhe Information Technology Co. Ltd    

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return