Volume 39 Issue 6
Jun.  2023
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Gu C,Cao GB,Zhang ZQ,et al.Effects of tensile force on the vascular lumen formation in three-dimensional printed tissue[J].Chin J Burns Wounds,2023,39(6):565-572.DOI: 10.3760/cma.j.cn501225-20220903-00377.
Citation: Gu C,Cao GB,Zhang ZQ,et al.Effects of tensile force on the vascular lumen formation in three-dimensional printed tissue[J].Chin J Burns Wounds,2023,39(6):565-572.DOI: 10.3760/cma.j.cn501225-20220903-00377.

Effects of tensile force on the vascular lumen formation in three-dimensional printed tissue

doi: 10.3760/cma.j.cn501225-20220903-00377
Funds:

Youth Science Foundation Program of National Natural Science Foundation of China 31900969

General Program of Natural Science Foundation of Jiangsu Province of China BK20221245

Suzhou Municipal Science and Technology Plan Program SKY2022087

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  •   Objective   To explore the effects of tensile force on vascular lumen formation in three-dimensional printed tissue.   Methods   The experimental research method was used. Human umbilical vein endothelial cells (HUVECs) were extracted from discarded umbilical cord tissue of 3 healthy women (aged 22 to 35 years) who gave birth in the Department of Gynaecology and Obstetrics of Suzhou Ruihua Orthopaedic Hospital from September 2020 to May 2021. Human skin fibroblasts (HSFs) were extracted from discarded normal skin tissue of 10 male patients (aged 20 to 45 years) who underwent wound repair in the Department of Hand Surgery of Suzhou Ruihua Orthopaedic Hospital from September 2020 to September 2022. After identification of the two kinds of cells, the 4 th to 6 th passage of cells were taken for the follow-up experiments. HUVECs and HSFs were used as seed cells, and polycaprolactone, gelatin, hyaluronic acid, and fibrin were used as scaffold materials, and the three-dimensional printed vascularized tissue was created by three-dimensional bioprinting technology. The printed tissue with polycaprolactone scaffold of 6 and 10 mm spacing, and without polycaprolactone scaffold were set as 6 mm spacing polycaprolactone group, 10 mm spacing polycaprolactone group, and non-polycaprolactone group, respectively. After 4 days of culture, the printed tissue in 10 mm spacing polycaprolactone group was selected to detect the cell survival by cell viability detection kit, and the cell survival rate was calculated. After 14 days of culture, the printed tissue in three groups were taken, and the shape change of tissue was observed by naked eyes; immunofluorescence staining was performed to observe the arrangement of filamentous actin, and lumen diameter, total length, and number of branches of vessel in the tissue. The tissue with micro-spring structure in the above-mentioned three groups was designed, printed, and cultured for 9 days, and the tensile force applied in the printed tissue was measured according to the force-displacement curve. The number of samples was all 3 in the above experiments. Data were statistically analyzed with one-way analysis of variance and Tukey test.   Results   After 4 days of culture, the cell survival rate in printed tissue in 10 mm spacing polycaprolactone group was (91.3±2.2)%. After 14 days of culture, the shape change of printed tissue in non-polycaprolactone group was not obvious, while the shape changes of printed tissue in 6 mm spacing polycaprolactone group and 10 mm spacing polycaprolactone group were obvious. After 14 days of culture, the arrangement of filamentous actin in the printed tissue in non-polycaprolactone group had no specific direction, while the arrangement of filamentous actin in the printed tissue in 6 mm spacing polycaprolactone group and 10 mm spacing polycaprolactone group had a specific direction. After 14 days of culture, The vascular lumen diameters of the printed tissue in 6 mm spacing polycaprolactone group and 10 mm spacing polycaprolactone group were (6.0±1.3) and (10.8±1.3) μm, respectively, which were significantly larger than 0 μm in non-polycaprolactone group ( P<0.05), and the vascular lumen diameter of printed tissue in 10 mm spacing polycaprolactone group was significantly larger than that in 6 mm spacing polycaprolactone group ( P<0.05); the total length and number of branches of blood vessel in the printed tissue in 6 mm spacing polycaprolactone group and 10 mm spacing polycaprolactone group were significantly shorter or less than those in non-polycaprolactone group ( P<0.05), and the total length and number of branches of blood vessel in the printed tissue in 10 mm spacing polycaprolactone group were significantly shorter or less than those in 6 mm spacing polycaprolactone group. After 9 days of culture, the tensile forces applied in the printed tissue in 6 mm spacing polycaprolactone group and 10 mm spacing polycaprolactone group were (2 340±59) and (4 284±538) μN, respectively, which were significantly higher than 0 μN in non-polycaprolactone group ( P<0.05), and the tensile force applied in the printed tissue in 10 mm spacing polycaprolactone group was significantly higher than that in 6 mm spacing polycaprolactone group ( P<0.05).   Conclusions   The three-dimensional printed scaffold structure can exert different tensile force in the printed tissue, and the vascular lumen diameter of the printed tissue can be regulated by adjusting the tensile force.

     

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  • [1]
    StinnerDJ,EdwardsD.Surgical management of musculoskeletal trauma[J].Surg Clin North Am,2017,97(5):1119-1131.DOI: 10.1016/j.suc.2017.06.005.
    [2]
    XuJ,YinL,CaoS,et al.Application of WALANT technique for repairing finger skin defect with a random skin flap[J].J Orthop Surg Res,2021,16(1):164.DOI: 10.1186/s13018-021-02319-3.
    [3]
    周荣,巨积辉,唐林峰,等.带感觉神经的股前外侧穿支皮瓣修复足底皮肤软组织缺损的临床效果[J].中华烧伤杂志,2021,37(5):453-459.DOI: 10.3760/cma.j.cn501120-20200309-00136.
    [4]
    黄广涛,魏在荣,黄丽,等.二纵三横法在胸背动脉穿支皮瓣穿支定位及深度创面修复中的临床应用效果[J].中华烧伤与创面修复杂志,2022,38(2):165-169.DOI: 10.3760/cma.j.cn501120-20201207-00519.
    [5]
    戚建武,陈邵,孙斌鸿,等.游离背阔肌肌皮瓣联合人工真皮及刃厚皮治疗下肢脱套伤的临床效果[J].中华烧伤与创面修复杂志,2022,38(4):347-353.DOI: 10.3760/cma.j.cn501120-20210421-00145.
    [6]
    HerskovitzI,HughesOB,MacquhaeF,et al.Epidermal skin grafting[J].Int Wound J,2016,13 Suppl 3:S52-56.DOI: 10.1111/iwj.12631.
    [7]
    肖仕初,郑勇军.组织工程皮肤现状与挑战[J].中华烧伤杂志,2020,36(3):166-170.DOI: 10.3760/cma.j.cn501120-20191202-00449.
    [8]
    De DeckerI, HoeksemaH, VerbelenJ, et al. A single-stage bilayered skin reconstruction using Glyaderm® as an acellular dermal regeneration template results in improved scar quality: an intra-individual randomized controlled trial[J/OL]. Burns Trauma,2023,11:tkad015[2023-05-24].https://pubmed.ncbi.nlm.nih.gov/37143955/.DOI: 10.1093/burnst/tkad015.
    [9]
    VigK,ChaudhariA,TripathiS,et al.Advances in skin regeneration using tissue engineering[J].Int J Mol Sci,2017,18(4):789. DOI: 10.3390/ijms18040789.
    [10]
    王晓静,王国伟,惠光艳,等.组织工程化皮肤:从形态和功能安全替代的前景[J].中国组织工程研究,2017,21(16):2600-2605.DOI: 10.3969/j.issn.2095-4344.2017.16.024.
    [11]
    DattaP,AyanB,OzbolatIT.Bioprinting for vascular and vascularized tissue biofabrication[J].Acta Biomater,2017,51:1-20.DOI: 10.1016/j.actbio.2017.01.035.
    [12]
    DimitrievskaS,NiklasonLE.Historical perspective and future direction of blood vessel developments[J].Cold Spring Harb Perspect Med,2018,8(2):a025742. DOI: 10.1101/cshperspect.a025742.
    [13]
    RosenfeldD,LandauS,ShandalovY,et al.Morphogenesis of 3D vascular networks is regulated by tensile forces[J].Proc Natl Acad Sci U S A,2016,113(12):3215-3220.DOI: 10.1073/pnas.1522273113.
    [14]
    WenZ,ZhouH,ZhouJ,et al.Quantitative evaluation of mechanical stimulation for tissue-engineered blood vessels[J].Tissue Eng Part C Methods,2021,27(5):337-347.DOI: 10.1089/ten.TEC.2021.0007.
    [15]
    WangX,LiX,LiJ,et al.Mechanical loading stimulates bone angiogenesis through enhancing type H vessel formation and downregulating exosomal miR-214-3p from bone marrow-derived mesenchymal stem cells[J].FASEB J,2021,35(1):e21150.DOI: 10.1096/fj.202001080RR.
    [16]
    ZhangG, CaoG, GuC, et al. Regulation of vascular branch formation in 3D bioprinted tissues using confining force[J]. Appl Mater Today, 2022, 26:101240.DOI: 10.1016/j.apmt.2021.101240.
    [17]
    曹高标 CDC42在牵张力调控3D打印组织血管分支形成中的作用研究 扬州 扬州大学 2022 DOI: 10.27441/d.cnki.gyzdu.2022.001640

    曹高标.CDC42在牵张力调控3D打印组织血管分支形成中的作用研究[D].扬州:扬州大学,2022.DOI:10.27441/d.cnki.gyzdu.2022.001640.

    [18]
    林苗远,李豫皖,刘毅,等.组织工程皮肤的研究热点及应用价值[J].中国组织工程研究,2022,26(1):153-159.
    [19]
    MeuliM,Hartmann-FritschF,HügingM,et al.A cultured autologous dermo-epidermal skin substitute for full-thickness skin defects: a phase I, open, prospective clinical trial in children[J].Plast Reconstr Surg,2019,144(1):188-198.DOI: 10.1097/PRS.0000000000005746.
    [20]
    AugerFA,GibotL,LacroixD.The pivotal role of vascularization in tissue engineering[J].Annu Rev Biomed Eng,2013,15:177-200.DOI: 10.1146/annurev-bioeng-071812-152428.
    [21]
    Ben-ShaulS,LandauS,MerdlerU,et al.Mature vessel networks in engineered tissue promote graft-host anastomosis and prevent graft thrombosis[J].Proc Natl Acad Sci U S A,2019,116(8):2955-2960.DOI: 10.1073/pnas.1814238116.
    [22]
    HomaeigoharS, LiM, BoccacciniAR. Bioactive glass-based fibrous wound dressings[J/OL]. Burns Trauma,2022,10:tkac038[2023-05-24].https://pubmed.ncbi.nlm.nih.gov/36196303/. DOI: 10.1093/burnst/tkac038.
    [23]
    DimopoulosA,MarkatosDN,MitropoulouA,et al.A novel polymeric fibrous microstructured biodegradable small-caliber tubular scaffold for cardiovascular tissue engineering[J].J Mater Sci Mater Med,2021,32(2):21.DOI: 10.1007/s10856-021-06490-1.
    [24]
    KoleskyDB,HomanKA,Skylar-ScottMA,et al.Three-dimensional bioprinting of thick vascularized tissues[J].Proc Natl Acad Sci U S A,2016,113(12):3179-3184.DOI: 10.1073/pnas.1521342113.
    [25]
    李惠杰,徐雷,钟葳珍,等.光固化3D打印药物制剂技术研究进展[J].中国药学杂志,2021,56(15):1189-1195.DOI: 10.11669/cpj.2021.15.001.
    [26]
    ChenJ,AlexanderGC,BobbaPS,et al.Recent progress in vascular tissue-engineered blood vessels[J].Adv Exp Med Biol,2018,1064:123-144.DOI: 10.1007/978-981-13-0445-3_8.
    [27]
    VukicevicM,MosadeghB,MinJK,et al.Cardiac 3D printing and its future directions[J].JACC Cardiovasc Imaging,2017,10(2):171-184.DOI: 10.1016/j.jcmg.2016.12.001.
    [28]
    ZhangG,VarkeyM,WangZ,et al.ECM concentration and cell-mediated traction forces play a role in vascular network assembly in 3D bioprinted tissue[J].Biotechnol Bioeng,2020,117(4):1148-1158.DOI: 10.1002/bit.27250.
    [29]
    ZhangG,WangZ,HanF,et al.Mechano-regulation of vascular network formation without branches in 3D bioprinted cell-laden hydrogel constructs[J].Biotechnol Bioeng,2021,118(10):3787-3798.DOI: 10.1002/bit.27854.
    [30]
    BoardmanR,PangV,MalhiN,et al.Activation of Notch signaling by soluble Dll4 decreases vascular permeability via a cAMP/PKA-dependent pathway[J].Am J Physiol Heart Circ Physiol,2019,316(5):H1065-H1075.DOI: 10.1152/ajpheart.00610.2018.
    [31]
    FouilladeC,Monet-LeprêtreM,Baron-MenguyC,et al.Notch signalling in smooth muscle cells during development and disease[J].Cardiovasc Res,2012,95(2):138-146.DOI: 10.1093/cvr/cvs019.
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