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Volume 40 Issue 8
Aug.  2024
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Article Navigation >  CHINESE JOURNAL OF BURNS AND WOUNDS  > 2024  >  40(8): 746-755
Li CY,Zhao XG,Hao JW,et al.Characteristics of renal oxidative stress injuries in rats with high-voltage electric burns and the intervention effects of breviscapine[J].Chin J Burns Wounds,2024,40(8):746-755.DOI: 10.3760/cma.j.cn501225-20240222-00070.
Citation: Li CY,Zhao XG,Hao JW,et al.Characteristics of renal oxidative stress injuries in rats with high-voltage electric burns and the intervention effects of breviscapine[J].Chin J Burns Wounds,2024,40(8):746-755.DOI: 10.3760/cma.j.cn501225-20240222-00070.
shu

Characteristics of renal oxidative stress injuries in rats with high-voltage electric burns and the intervention effects of breviscapine

doi: 10.3760/cma.j.cn501225-20240222-00070

  • Burn and Wound Repair Center, the Third Hospital of Hebei Medical University, Hebei Burn Treatment Technology Innovation Center, Shijiazhuang 050051, China

Funds:

General Program of Natural Science Foundation of Hebei Province of China H2024206495

Hebei Province Innovation Capability Enhancement Plan Project 225676120H

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    Abstract
  •   Objective  To explore the characteristics of renal oxidative stress injuries in rats with high-voltage electric burns and the intervention effects of breviscapine.  Methods  This study was an experimental study. One hundred and sixty 8-10-week-old male Sprague Dawley rats were divided into sham injury group, electric burn group, saline group, low breviscapine group, middle breviscapine group, and high breviscapine group, with 60 rats in each of the sham injury group and electric burn group, 10 rats in each of the other 4 groups, respectively. The rats in sham injury group and electric burn group were divided into 10 rats at each time point, including post injury hour (PIH) 0 (immediately), 8, 24, 48, and 72, and post injury week (PIW) 1. The rats in sham injury group were not conducted with electrical current to cause sham injury. The rats in the other 5 groups were caused high-voltage electric burns. The rats in sham injury group and electric burn group were not treated after injury. The rats in saline group, low breviscapine group, middle breviscapine group, and high breviscapine group were intraperitoneally injected with 5 mL/kg normal saline or 0.4, 1.6, and 4.0 g/L breviscapine, repeated every 24 h until PIH 72. After the model was successfully made, 14 rats died, including 1, 2, 2, and 1 rat (s) at PIH 24, 48, and 72 and PIW 1 in electric burn group, 4, 1, 2, and 1 rat (s) at PIH 72 in saline group, low breviscapine group, middle breviscapine group, and high breviscapine group, respectively. The kidney tissue collected from rats in the 6 groups was weighed and the kidney/body weight ratio was calculated. The left upper pole tissue of kidney was collected from each 4 rats in sham injury group, and in electric burn group at PIH 8, 24, 48, and 72 and PIW 1, and in saline group, low breviscapine group, middle breviscapine group, and high breviscapine group at PIH 72. The renal tubular and renal interstitial injury was evaluated by a semi-quantitative histological scoring system after hematoxylin-eosin staining. The inferior vena cava blood samples were collected from rats in the 6 groups to measure the serum creatinine levels via sarcosine oxidase method, and serum urea nitrogen levels via urease method. The right renal cortices were collected from rats in the 6 groups to measure the catalase (CAT) activity in the supernatant of renal tissue via molybdic acid method, and the levels of advanced oxidation protein product (AOPP) and Klotho protein in the supernatant of renal tissue via enzyme-linked immunosorbent assay.  Results  At PIH 8, 48, and 72 and PIW 1, the kidney/body weight ratios of rats in electric burn group were significantly higher than those in sham injury group (with t values of -0.52, -3.75, -4.05, and -2.25,respectively, P<0.05). At PIH 72, compared with those in electric burn group, saline group, low breviscapine group, and middle breviscapine group, the kidney/body weight ratio of rats in high breviscapine group was significantly decreased (with P values all <0.05). Compared with those in sham injury group, the renal tubular and renal interstitial injury scores of rats in electric burn group at PIH 48 and 72 and PIW 1 were significantly increased (P<0.05). Compared with those in electric burn group at PIH 8 and 24, the renal tubular and renal interstitial injury score of rats in electric burn group at PIW 1 was significantly increased (with P values all <0.05). At PIH 72, the renal tubular and renal interstitial injury scores of rats in the 5 groups of rats with electric burns were similar (P>0.05). At PIH 8, 24, 48, and 72 and PIW 1, the levels of serum creatinine and serum urea nitrogen of rats in electric burn group were significantly higher than those in sham injury group (with Z values of -2.00, -2.37, -2.62, -2.67, -3.67, -2.34, -3.11, -3.43, -3.11, and -3.51, respectively, P<0.05). Compared with that in electric burn group at PIH 0, the levels of serum creatinine of rats in electric burn group at PIH 72 and PIW 1 were significantly increased (P<0.05). Compared with that in electric burn group at PIH 8, the levels of serum creatinine of rats in electric burn group at PIH 72 and PIW 1 were significantly increased (P<0.05). Compared with that in electric burn group at PIH 24, the level of serum creatinine of rats in electric burn group at PIW 1 was significantly increased (P<0.05). At PIH 72, the levels of serum creatinine of rats in the 5 groups of rats with electric burns were similar (P>0.05). Compared with that in electric burn group, the levels of serum urea nitrogen of rats in low breviscapine group, middle breviscapine group, and high breviscapine group were significantly decreased (P<0.05). Compared with that in saline group, the levels of serum urea nitrogen in middle breviscapine group and high breviscapine group were significantly decreased (P<0.05). At PIH 48 and 72 and PIW 1, the CAT activities in the supernatant of renal tissue of rats in electric burn group were significantly lower than those in sham injury group (with Z values of -2.22, -2.13, and -3.51, respectively, P<0.05). At PIH 8, 24, 48, and 72 and PIW 1, the levels of AOPP in the supernatant of renal tissue of rats in electric burn group were significantly higher than those in sham injury group (with Z values of -2.00, -3.15, -2.71, -2.04, and -2.33, respectively, P<0.05). At PIH 0-PIW 1, the levels of Klotho protein in the supernatant of renal tissue of rats in sham injury group and electric burn group were all similar (P>0.05). Compared with that in electric burn group at PIH 0, the CAT activities in the supernatant of renal tissue of rats in electric burn group at PIH 72 and PIW 1 and the levels of Klotho protein in the supernatant of renal tissue of rats in electric burn group at PIH 48 and 72 and PIW 1 were significantly decreased (P<0.05). Compared with that in electric burn group at PIH 8, the CAT activities in the supernatant of renal tissue of rats in electric burn group at PIH 72 and PIW 1 and the levels of Klotho protein in the supernatant of renal tissue of rats in electric burn group at PIH 48 and 72 and PIW 1 were significantly decreased (P<0.05). Compared with that in electric burn group at PIH 24, the CAT activities in the supernatant of renal tissue of rats in electric burn group at PIH 72 and PIW 1 were significantly decreased (P<0.05). Compared with that in electric burn group at PIH 48, the CAT activity in the supernatant of renal tissue of rats in electric burn group at PIW 1 was significantly decreased (P<0.05). At PIH 72, the levels of Klotho protein in the supernatant of renal tissue of rats in the 5 groups of rats with electric burns were similar (P<0.05). Compared with 14.6 (12.6, 23.6) U/mgprot in electric burn group, the CAT activities in the supernatant of renal tissue of rats in low breviscapine group (20.5 (18.0, 39.8) U/mgprot), middle breviscapine group (24.9 (14.7, 28.9) U/mgprot), and high breviscapine group (28.0 (21.9, 39.1) U/mgprot) were significantly increased (P<0.05). Compared with 15.7 (13.7, 25.6) U/mgprot in saline group, the CAT activities in the supernatant of renal tissue of rats in middle breviscapine group and high breviscapine group were significantly increased (P<0.05). Compared with that in low breviscapine group, the CAT activity in the supernatant of renal tissue of rats in high breviscapine group was significantly increased (P<0.05). Compared with those in electric burn group and saline group, the levels of AOPP in the supernatant of renal tissue of rats in middle breviscapine group and high breviscapine group were significantly decreased (P<0.05).  Conclusions  After high-voltage electric burns, oxidative stress injury occur in the kidneys of rats, which is aggravated with time extension. Breviscapine can alleviate oxidative stress injuries in the kidneys of rats with high-voltage electric burns.

     

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    • References(35)
  • [1]
    蒋梅君,李泽,谢卫国.2 133例电烧伤住院患者流行病学调查[J].中华烧伤杂志,2017,33(12):732-737. DOI: 10.3760/cma.j.issn.1009-2587.2017.12.003.
    [2]
    SchweizerR, PedrazziN, KleinHJ, et al. Risk factors for mortality and prolonged hospitalization in electric burn injuries[J]. J Burn Care Res, 2021,42(3):505-512. DOI: 10.1093/jbcr/iraa192.
    [3]
    KymD,SeoDK,HurGY,et al.Epidemiology of electrical injury: differences between low- and high-voltage electrical injuries during a 7-year study period in South Korea[J].Scand J Surg,2015,104(2):108-114.DOI: 10.1177/1457496914534209.
    [4]
    GurbuzK, DemirM. Patterns and outcomes of high-voltage vs low-voltage pediatric electrical injuries: an 8-year retrospective analysis of a tertiary-level burn center[J]. J Burn Care Res, 2022,43(3):704-709. DOI: 10.1093/jbcr/irab178.
    [5]
    DingH,HuangM,LiD,et al.Epidemiology of electrical burns: a 10-year retrospective analysis of 376 cases at a burn centre in South China[J].J Int Med Res,2020,48(3):300060519891325.DOI: 10.1177/0300060519891325.
    [6]
    张庆富,李勇,冯建科,等.高压电烧伤大鼠血小板流变行为变化及乌司他丁的干预效果[J].中华烧伤杂志,2017,33(12):744-749.DOI: 10.3760/cma.j.issn.1009-2587.2017.12.005.
    [7]
    孙斯琴,郭威,陈军,等.兔肢体高压电烧伤后骨骼肌MRI特征[J].放射学实践,2022,37(5):605-610.DOI: 10.13609/j.cnki.1000-0313.2022.05.014.
    [8]
    张庆富,高志娟,张紫薇,等.高压电烧伤对大鼠血清血小板相关因子及血小板聚集数的影响及血必净的干预作用[J].中华烧伤杂志,2020,36(6):426-432.DOI: 10.3760/cma.j.cn501120-20200407-00212.
    [9]
    LvY,ZhaoX,ZhangR,et al.Oxidative stress mediates hippocampal neuronal apoptosis through ROS/JNK/P53 pathway in rats with PTSD triggered by high-voltage electrical burn[J/OL].Folia Morphol (Warsz),2023(2023-09-11)[2024-02-22].https://pubmed.ncbi.nlm.nih.gov/37691512/.DOI:10.5603/fm.95727.[published online ahead of print].
    [10]
    鲁梦远,赵学刚,郝嘉文,等. 高压电烧伤大鼠肝脏氧化应激损伤及灯盏花素的干预作用[J/CD]. 中华损伤与修复杂志(电子版),2024,19(2):113-118. DOI: 10.3877/cma.j.issn.1673-9450.2024.02.004.
    [11]
    杨文飞,郝嘉文,鲁梦远,等.高压电烧伤对大鼠心肌氧化应激的影响及N-乙酰半胱氨酸的干预作用[J/CD].中华损伤与修复杂志(电子版),2024,19(2):102-108.DOI: 10.3877/cma.j.issn.1673-9450.2024.02.003.
    [12]
    闫聚瀚,赵朋涛,闫晓冬,等.小鼠肾缺血再灌注损伤模型的研究进展[J].西部医学,2023,35(10):1555-1560.DOI: 10.3969/j.issn.1672-3511.2023.10.029.
    [13]
    HeM,XueZM,LiJ,et al.Breviscapine inhibits high glucose-induced proliferation and migration of cultured vascular smooth muscle cells of rats via suppressing the ERK1/2 MAPK signaling pathway[J].Acta Pharmacol Sin,2012,33(5):606-614.DOI: 10.1038/aps.2012.6.
    [14]
    马华林,林海雁,徐莹,等.人脐带间充质干细胞延缓大鼠肾脏衰老的作用[J].暨南大学学报(自然科学与医学版),2018,39(1):41-46.DOI: 10.11778/j.jdxb.2018.01.007.
    [15]
    余美荣,郭松雪,金荣华,等.虾青素对Ⅲ度烧伤大鼠急性肾损伤的作用及机制[J].中华烧伤杂志,2020,36(11):1050-1059.DOI: 10.3760/cma.j.cn501120-20200526-00287.
    [16]
    SiesH.Oxidative stress: a concept in redox biology and medicine[J].Redox Biol,2015,4:180-183.DOI: 10.1016/j.redox.2015.01.002.
    [17]
    何小龙,张兵,李峥,等.富氢盐水对严重高电压烧伤大鼠肾功能及肾组织病理变化的影响[J].中国临床研究,2019,32(7):874-878. DOI: 10.13429/j.cnki.cjcr.2019.07.003.
    [18]
    陈卓,胡光俊,褚志刚,等.丙泊酚通过减轻炎症反应和内质网应激缓解烧伤后急性肾损伤[J].华中科技大学学报(医学版),2022,51(6):786-790,804.DOI: 10.3870/j.issn.1672-0741.2022.06.007.
    [19]
    任广胜,胡善友,张和凤,等.血清胱抑素C联合血乳酸对脓毒症急性肾损伤早期诊断的价值[J].中国急救复苏与灾害医学杂志,2019,14(6):540-543.DOI: 10.3969/j.issn.1673-6966.2019.06.013.
    [20]
    SemenovichDS, PlotnikovEY, LukiyenkoEP, et al. Protective effect of D-panthenol in rhabdomyolysis-induced acute kidney injury[J]. Int J Mol Sci, 2022, 23(20):12273. DOI: 10.3390/ijms232012273.
    [21]
    LinQ,LiS,JiangN,et al.PINK1-parkin pathway of mitophagy protects against contrast-induced acute kidney injury via decreasing mitochondrial ROS and NLRP3 inflammasome activation[J].Redox Biol,2019,26:101254.DOI: 10.1016/j.redox.2019.101254.
    [22]
    王东,李娟,王艳.血清肌酐、尿素氮与胱抑素C水平检测与肾病综合征病情严重程度的关系研究[J].临床研究,2024,32(5):139-142.DOI: 10.12385/j.issn.2096-1278(2024)05-0139-04.
    [23]
    魏志权,苟巧,张伟.过氧化氢酶与肿瘤的关系[J].癌变.畸变.突变,2013,25(1):79-81.DOI: 10.3969/j.issn.1004-616x.2013.01.019.
    [24]
    刘婷婷,彭静,李重阳,等.麻黄素对小鼠心肌组织结构和总抗氧化能力、过氧化氢酶活力的影响[J].解剖学报,2016,47(4):516-520.DOI: 10.16098/j.issn.0529-1356.2016.04.014.
    [25]
    丁星星,王建刚,刘新宇,等.大黄素预处理对脓毒症大鼠急性肾损伤保护作用机制研究[J].山西中医,2023,39(11):57-61.DOI: 10.20002/j.issn.1000-7156.2023.11.022.
    [26]
    MertH, AçikkolS, Çalliİ, et al. Advanced oxidation protein product (AOPP) levels in second-and third-degree thermal burns[J]. J Burn Care Res, 2021, 42(2): 207-211. DOI: 10.1093/jbcr/iraa127.
    [27]
    CaoW,HouFF,NieJ.AOPPs and the progression of kidney disease[J].Kidney Int Suppl (2011),2014,4(1):102-106.DOI: 10.1038/kisup.2014.19.
    [28]
    Abdel-MagiedN,ElkadyAA.Possible curative role of curcumin and silymarin against nephrotoxicity induced by gamma-rays in rats[J].Exp Mol Pathol,2019,111:104299.DOI: 10.1016/j.yexmp.2019.104299.
    [29]
    XuY,SunZ.Molecular basis of Klotho: from gene to function in aging[J].Endocr Rev,2015,36(2):174-193.DOI: 10.1210/er.2013-1079.
    [30]
    HuMC,ShiMJ,ZhangJN,et al.Klotho deficiency is an early biomarker of renal ischemia-reperfusion injury and its replacement is protective[J].Kidney Int,2010,78(12):1240-1251.DOI: 10.1038/ki.2010.328.
    [31]
    徐正良,李德璇,李亚山,等.灯盏花素对铅中毒大鼠心肌细胞氧化应激损伤的影响[J].工业卫生与职业病,2023,49(5):385-388,410.DOI: 10.13692/j.cnki.gywsyzyb.2023.05.001.
    [32]
    赵丽雅,胡宏英,任利彬.灯盏花素对庆大霉素所致急性肾损伤的保护作用及机制研究[J].天津中医药,2019,36(6):603-607.DOI: 10.11656/j.issn.1672-1519.2019.06.21.
    [33]
    王静,李敏,何立明,等.灯盏花素保护大鼠免受糖尿病肾病侵害的作用机制研究[J].世界中医药,2022,17(22):3162-3167.DOI: 10.3969/j.issn.1673-7202.2022.22.007.
    [34]
    张明昊,赵绅,杜婧雯,等.灯盏花素通过调控TGF-β1介导的Smad和ERK通路干预肾纤维化大鼠的作用机制研究[J].中药新药与临床药理,2022,33(10):1347-1356.DOI: 10.19378/j.issn.1003-9783.2022.10.008.
    [35]
    刘向云,王永,李雷,等.注射用灯盏花素对大鼠和犬的毒性病理学比较[J].中国药理学与毒理学杂志,2013,27(6):1000-1006.DOI: 10.3867/j.issn.1000-3002.2013.06.014.
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