基于组织工程新技术构建理想体外瘢痕模型的研究进展

朱冬振; 姚斌; 闫自强; 黄沙; 付小兵

doi:10.3760/cma.j.cn501120-20210723-00257

基于组织工程新技术构建理想体外瘢痕模型的研究进展

doi: 10.3760/cma.j.cn501120-20210723-00257

1.
解放军总医院医学创新研究部创伤修复与组织再生研究中心，北京　　100048
2.
天津大学医学工程与转化医学研究院，天津　　300072
3.
解放军69213部队，喀什　　844900

基金项目:

国家重点研发计划 2017YFC1103303

国家自然科学基金青年科学基金项目 32000969

上海王正国创伤医学发展基金会生长因子复兴计划 SZYZ-TR-03

详细信息

通讯作者:
黄沙，Email：stellarahuang@sina.com

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出版历程
- 收稿日期: 2021-07-23
- 网络出版日期: 2022-10-24

Research advances on the construction of an ideal scar model in vitro based on innovative tissue engineering technology

1.
Research Center for Wound Repair and Tissue Regeneration, Medical Innovation Research Department, the PLA General Hospital, Beijing 100048, China
2.
Institute of Medical Engineering and Translational Medicine, Tianjin University, Tianjin 300072, China
3.
Unit 69213 of PLA, Kashgar 844900, China

Funds:

National Key Research and Development Program of China 2017YFC1103303

Youth Science Foundation of National Natural Science Foundation of China 32000969

Shanghai Wang Zhengguo Foundation for Traumatic Medicine Growth Factor Rejuvenation Plan SZYZ-TR-03

More Information

Corresponding author: Huang Sha, Email: stellarahuang@sina.com

摘要

摘要: 瘢痕的形成给患者造成巨大的经济负担和严重的心理阴影。尽管目前用于瘢痕治疗的手段趋于多样化，但是能够真正实现人体皮肤损伤后的“完美愈合”或是“无瘢痕愈合”的治疗方法相当匮乏。随着组织工程技术在医学研究中的广泛应用，诸如生物三维打印、类器官培养和器官芯片技术等新技术不断涌现，基于这些新技术构建的体外疾病模型也展现出比以往传统动物疾病模型更大的优势。该文介绍了类器官培养、生物三维打印、器官芯片技术等目前在皮肤组织工程中应用的热点技术，重点总结了构建理想的体外瘢痕模型需把握的3个关键要素，并结合该研究团队长期从事皮肤组织修复与再生研究的经验，对未来构建理想体外瘢痕模型进行展望。
- 瘢痕 /
- 组织工程 /
- 皮肤 /
- 疾病模型
Abstract: The scar brings a huge economic burden and creates a serious psychological shadow for patients. Although the current methods for scar treatment tend to be diversified, the treatment method that can truly achieve the goal of "perfect healing" or "scarless healing" after human skin injury is quite scarce. With the wide application of tissue engineering technologies in medicine research, technologies such as three-dimensional bioprinting, organoid culture, and organ chip technologies are constantly emerging. Disease models in vitro based on these innovative technologies showed more advantages than traditional animal disease models. The article introduces the current hotspot technologies in skin tissue engineering such as organoid culture, three-dimensional bioprinting, and organ chip technologies, focuses on summarizing the three key elements to be mastered for constructing an ideal scar model in vitro, and puts forward the future prospect of constructing an ideal scar model in vitro based on our research team's long-term experience in skin tissue repair and regeneration research.
- Cicatrix /
- Tissue engineering /
- Skin /
- Disease model
所有作者均声明不存在利益冲突

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表 1 皮肤组织工程常用的生物三维打印方法的优点和不足

打印方法	优点	不足
喷墨式打印	（1）打印速度快	（1）需采用有一定黏滞性的材料
	（2）可采用多种聚合机制	（2）不能打印厚度较高的模型
	（3）低成本、可商业化	（3）打印材料中细胞密度较低
		（4）打印过程影响细胞活性
微挤出式打印	（1）可采用高黏滞性和高细胞密度溶液打印	（1）喷头易阻塞
	（2）可打印较厚的垂直结构	（2）高精度打印影响细胞活性
	（3）可采用多种聚合机制	（3）各层连接松散
激光辅助式打印	（1）打印材料中细胞活性高	（1）需采用有一定黏滞性的材料
	（2）高细胞密度打印	（2）不能打印厚度较高的模型
	（3）适用于低黏滞性材料	（3）单一聚合机制
	（4）打印精度高	（4）过程复杂、费用高
立体光刻式打印	（1）打印速度快	（1）打印墨水只能用光交联聚合物
	（2）打印精度高	（2）需采用有一定黏滞性的材料
	（3）可打印较厚的垂直结构	（3）紫外线可影响细胞活性
	（4）打印材料内部连接较为紧密

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