各种组学分析在体表慢性难愈合创面中的研究进展

曾帅丹; 杨磊

doi:10.3760/cma.j.cn501225-20220216-00030

各种组学分析在体表慢性难愈合创面中的研究进展

doi: 10.3760/cma.j.cn501225-20220216-00030

曾帅丹,
杨磊^,

南方医科大学南方医院烧伤科，广州　　510515

基金项目:

广东省重点领域研发计划项目 2020B1111150001

广东省自然科学基金项目 2020A151501108

广东省省级科技计划项目 2018KJYZ005

深圳市医疗卫生三名工程项目 SZSM202011012

详细信息

通讯作者:
杨磊，Email：yuanyang@smu.edu.cn

计量
- 文章访问数: 400
- HTML全文浏览量: 77
- PDF下载量: 78
- 被引次数: 0
出版历程
- 收稿日期: 2022-02-16

Research advances of various omics analyses in chronic refractory wounds on body surface

Zeng Shuaidan,
Yang Lei^,

Department of Burns, Nanfang Hospital, Southern Medical University, Guangzhou 510515, ChinaCorresponding auhtor: Yang Lei, Email: yuanyang@smu.edu.cn

Funds:

Guangdong Province Key Field R&D Program Project 2020B1111150001

Natural Science Foundation of Guangdong Province of China 2020A151501108

Science and Technology Innovation Project of Guangdong Province of China 2018KJYZ005

Sanming Project of Medicine in Shenzhen SZSM202011012

摘要

摘要: 体表慢性难愈合创面的诊断和治疗一直充满着挑战，对医疗和社会造成巨大负担。利用高通量测序技术结合组学分析可以揭示慢性创面形成的潜在机制，识别与慢性创面诊断、预后和筛查相关的潜在生物标志物。而联合多个水平的组学分析能进一步探索和理解其中详细的分子机制，为制订个性化治疗方法提供线索，为慢性创面的精准医疗打下坚实的基础。因此，该文针对近年各种组学分析在体表慢性难愈合创面相关研究中的进展进行综述。
- 精准医学 /
- 蛋白质组学 /
- 慢性创面 /
- 创面修复
Abstract: The diagnosis and treatment of chronic refractory wounds on body surface has always been full of challenges, and it also poses a huge burden on medical care and society. High-throughput sequencing combined with omics analysis can reveal potential mechanisms of chronic wound formation, and identify potential biomarkers related to diagnosis, prognosis, and screening of chronic wound. Combined with multiple levels of omics analysis, the detailed molecular mechanism of chronic wound development can be further explored and understood, so as to provide clues for the formulation of personalized treatment methods and lay a solid foundation for the precision medicine of chronic wounds. Therefore, this review addresses the recent progress of various omics analyses in chronic refractory wounds on body surface.
- Precision medicine /
- Proteomics /
- Chronic wounds /
- Wound repair
所有作者均声明不存在利益冲突

HTML全文

参考文献(52)

[1]	GravesN,PhillipsCJ,HardingK.A narrative review of the epidemiology and economics of chronic wounds[J].Br J Dermatol,2022,187(2):141-148.DOI: 10.1111/bjd.20692.
[2]	JonesRE,FosterDS,LongakerMT.Management of chronic wounds-2018[J].JAMA,2018,320(14):1481-1482.DOI: 10.1001/jama.2018.12426.
[3]	ShawTJ,MartinP.Wound repair: a showcase for cell plasticity and migration[J].Curr Opin Cell Biol,2016,42:29-37.DOI: 10.1016/j.ceb.2016.04.001.
[4]	OlivierM,AsmisR,HawkinsGA,et al.The need for multi-omics biomarker signatures in precision medicine[J].Int J Mol Sci,2019, 20(19):4781. DOI: 10.3390/ijms20194781.
[5]	LuM,ZhanX.The crucial role of multiomic approach in cancer research and clinically relevant outcomes[J].EPMA J,2018,9(1):77-102.DOI: 10.1007/s13167-018-0128-8.
[6]	董炜,肖玉瑞,吴敏洁,等.中国慢性难愈性创面诊疗思路及原则[J].中华烧伤杂志,2018,34(12):868-873.DOI: 10.3760/cma.j.issn.1009-2587.2018.12.010.
[7]	FrykbergRG,BanksJ.Challenges in the treatment of chronic wounds[J].Adv Wound Care (New Rochelle),2015,4(9):560-582.DOI: 10.1089/wound.2015.0635.
[8]	The Wound Healing SocietyChronic wound care guidelines2016-04-212022-02-16https://woundheal.org/Publications/WHS-Wound-Care-Guidelines.cgi The Wound Healing Society. Chronic wound care guidelines[EB/OL].(2016-04-21)[2022-02-16]. https://woundheal.org/Publications/WHS-Wound-Care-Guidelines.cgi.
[9]	廖新成,郭光华.慢性难愈性创面的分类鉴别及临床评估[J/CD].中华损伤与修复杂志:电子版,2017,12(4):303-305.DOI: 10.3877/cma.j.issn.1673-9450.2017.04.012.
[10]	BowersS,FrancoE.Chronic wounds: evaluation and management[J].Am Fam Physician,2020,101(3):159-166.
[11]	RaffettoJD.Pathophysiology of chronic venous disease and venous ulcers[J].Surg Clin North Am,2018,98(2):337-347.DOI: 10.1016/j.suc.2017.11.002.
[12]	SoyoyeDO,AbiodunOO,IkemRT,et al.Diabetes and peripheral artery disease: a review[J].World J Diabetes,2021,12(6):827-838.DOI: 10.4239/wjd.v12.i6.827.
[13]	HutchingsG,KruszynaŁ,NawrockiMJ,et al.Molecular mechanisms associated with ROS-dependent angiogenesis in lower extremity artery disease[J].Antioxidants (Basel),2021, 10(5):735. DOI: 10.3390/antiox10050735.
[14]	BandykDF.The diabetic foot: pathophysiology, evaluation, and treatment[J].Semin Vasc Surg,2018,31(2/3/4):43-48.DOI: 10.1053/j.semvascsurg.2019.02.001.
[15]	HeadlamJ,IllsleyA.Pressure ulcers: an overview[J].Br J Hosp Med (Lond),2020,81(12):1-9.DOI: 10.12968/hmed.2020.0074.
[16]	KwekM,ThangavelooM,HuiS,et al.Characterisation of an ischemia reperfusion model for the formation of a stage I pressure ulcer in mouse skin[J].J Tissue Viability,2021,30(3):352-362.DOI: 10.1016/j.jtv.2021.03.004.
[17]	KuroseT,HashimotoM,OzawaJ,et al.Analysis of gene expression in eExperimental pressure ulcers in the rat with special reference to inflammatory cytokines[J].PLoS One,2015,10(7):e0132622.DOI: 10.1371/journal.pone.0132622.
[18]	MirzaRE,FangMM,Weinheimer-HausEM,et al.Sustained inflammasome activity in macrophages impairs wound healing in type 2 diabetic humans and mice[J].Diabetes,2014,63(3):1103-1114.DOI: 10.2337/db13-0927.
[19]	MirzaR,KohTJ.Dysregulation of monocyte/macrophage phenotype in wounds of diabetic mice[J].Cytokine,2011,56(2):256-264.DOI: 10.1016/j.cyto.2011.06.016.
[20]	ParnhamA,BousfieldC.The influence of matrix metalloproteases and biofilm on chronic wound healing: a discussion[J].Br J Community Nurs,2018,23(Suppl 3):S22-29.DOI: 10.12968/bjcn.2018.23.Sup3.S22.
[21]	ZhangC,LimJ,JeonHH,et al.FOXO1 deletion in keratinocytes improves diabetic wound healing through MMP9 regulation[J].Sci Rep,2017,7(1):10565.DOI: 10.1038/s41598-017-10999-3.
[22]	StechmillerJ,CowanL,SchultzG.The role of doxycycline as a matrix metalloproteinase inhibitor for the treatment of chronic wounds[J].Biol Res Nurs,2010,11(4):336-344.DOI: 10.1177/1099800409346333.
[23]	KathawalaMH,NgWL,LiuD,et al.Healing of chronic wounds: an update of recent developments and future possibilities[J].Tissue Eng Part B Rev,2019,25(5):429-444.DOI: 10.1089/ten.TEB.2019.0019.
[24]	温学良,荣新洲.慢性创面治疗新进展[J/CD].中华损伤与修复杂志:电子版,2018,13(4):308-311.DOI: 10.3877/cma.j.issn.1673-9450.2018.04.013.
[25]	郝擎宇,葛乃航,宋德恒,等.慢性难愈性创面治疗方法的研究进展[J].感染、炎症、修复,2017,18(3):186-189.DOI: 10.3969/j.issn.1672-8521.2017.03.017.
[26]	GouldL,StuntzM,GiovannelliM,et al.Wound Healing Society 2015 update on guidelines for pressure ulcers[J].Wound Repair Regen,2016,24(1):145-162.DOI: 10.1111/wrr.12396.
[27]	ChinL,AndersenJN,FutrealPA.Cancer genomics: from discovery science to personalized medicine[J].Nat Med,2011,17(3):297-303.DOI: 10.1038/nm.2323.
[28]	EckhardU,MarinoG,ButlerGS,et al.Positional proteomics in the era of the human proteome project on the doorstep of precision medicine[J].Biochimie,2016,122:110-118.DOI: 10.1016/j.biochi.2015.10.018.
[29]	DuarteTT,SpencerCT.Personalized proteomics: the future of precision medicine[J].Proteomes,2016,4(4):29.DOI: 10.3390/proteomes4040029.
[30]	EverettJR.NMR-based pharmacometabonomics: a new paradigm for personalised or precision medicine[J].Prog Nucl Magn Reson Spectrosc,2017,102-103:1-14.DOI: 10.1016/j.pnmrs.2017.04.003.
[31]	AdilA,KumarV,JanAT,et al.Single-cell transcriptomics: current methods and challenges in data acquisition and analysis[J].Front Neurosci,2021,15:591122.DOI: 10.3389/fnins.2021.591122.
[32]	CollinsFS,VarmusH.A new initiative on precision medicine[J].N Engl J Med,2015,372(9):793-795.DOI: 10.1056/NEJMp1500523.
[33]	PatelS,MaheshwariA,ChandraA.Biomarkers for wound healing and their evaluation[J].J Wound Care,2016,25(1):46-55.DOI: 10.12968/jowc.2016.25.1.46.
[34]	StoneRC,ChenV,BurgessJ,et al.Genomics of human fibrotic diseases: disordered wound healing response[J].Int J Mol Sci,2020,21 (22):8590.DOI: 10.3390/ijms21228590.
[35]	FuH,ZhouH,YuX,et al.Wounding triggers MIRO-1 dependent mitochondrial fragmentation that accelerates epidermal wound closure through oxidative signaling[J].Nat Commun,2020,11(1):1050.DOI: 10.1038/s41467-020-14885-x.
[36]	ScottiMM,SwansonMS.RNA mis-splicing in disease[J].Nat Rev Genet,2016,17(1):19-32.DOI: 10.1038/nrg.2015.3.
[37]	JamesGA,Ge ZhaoA,UsuiM,et al.Microsensor and transcriptomic signatures of oxygen depletion in biofilms associated with chronic wounds[J].Wound Repair Regen,2016,24(2):373-383.DOI: 10.1111/wrr.12401.
[38]	BeelerJS,MarshallCB,Gonzalez-EricssonPI,et al.p73 regulates epidermal wound healing and induced keratinocyte programming[J].PLoS One,2019,14(6):e0218458.DOI: 10.1371/journal.pone.0218458.
[39]	LiD,ChengS,PeiY,et al.Single-cell analysis reveals major histocompatibility complex Ⅱ-expressing keratinocytes in pressure ulcers with worse healing outcomes[J].J Invest Dermatol,2022,142(3 Pt A):705-716.DOI: 10.1016/j.jid.2021.07.176.
[40]	YueL,ZhangF,SunR,et al.Generating proteomic big data for precision medicine[J].Proteomics,2020,20(21/22):e1900358.DOI: 10.1002/pmic.201900358.
[41]	FernandezML,BroadbentJA,ShooterGK,et al.Development of an enhanced proteomic method to detect prognostic and diagnostic markers of healing in chronic wound fluid[J].Br J Dermatol,2008,158(2):281-290.DOI: 10.1111/j.1365-2133.2007.08362.x.
[42]	BerberichB,ThrieneK,GretzmeierC,et al.Proteomic profiling of fibroblasts isolated from chronic wounds identifies disease-relevant signaling pathways[J].J Invest Dermatol,2020,140(11):2280-2290.e4.DOI: 10.1016/j.jid.2020.02.040.
[43]	BealeDJ,PinuFR,KouremenosKA,et al.Review of recent developments in GC-MS approaches to metabolomics-based research[J].Metabolomics,2018,14(11):152.DOI: 10.1007/s11306-018-1449-2.
[44]	NicholsonJK,LindonJC,HolmesE."Metabonomics": understanding the metabolic responses of living systems to pathophysiological stimuli via multivariate statistical analysis of biological NMR spectroscopic data[J].Xenobiotica,1999,29(11):1181-1189.DOI: 10.1080/004982599238047.
[45]	JunkaA,WojtowiczW,ZąbekA,et al.Metabolic profiles of exudates from chronic leg ulcerations[J].J Pharm Biomed Anal,2017,137:13-22.DOI: 10.1016/j.jpba.2017.01.018.
[46]	CzajkowskaJ,JunkaA,HoppeJ,et al.The co-culture of staphylococcal biofilm and fibroblast cell line: the correlation of biological phenomena with metabolic NMR1 footprint[J].Int J Mol Sci,2021,22(11):5826. DOI: 10.3390/ijms22115826.
[47]	RamirezHA,LiangL,PastarI,et al.Comparative genomic, microRNA, and tissue analyses reveal subtle differences between non-diabetic and diabetic foot skin[J].PLoS One,2015,10(8):e0137133.DOI: 10.1371/journal.pone.0137133.
[48]	IcliB,WuW,OzdemirD,et al.MicroRNA-135a-3p regulates angiogenesis and tissue repair by targeting p38 signaling in endothelial cells[J].FASEB J,2019,33(4):5599-5614.DOI: 10.1096/fj.201802063RR.
[49]	ZhongH,QianJ,XiaoZ,et al.MicroRNA-133b inhibition restores EGFR expression and accelerates diabetes-impaired wound healing[J].Oxid Med Cell Longev,2021,2021:9306760.DOI: 10.1155/2021/9306760.
[50]	JanuszykM,ChenK,HennD,et al.Characterization of diabetic and non-diabetic foot ulcers using single-cell RNA-sequencing[J].Micromachines (Basel),2020,11(9):815.DOI: 10.3390/mi11090815.
[51]	TheocharidisG, BaltzisD, RoustitM, et al. Integrated skin transcriptomics and serum multiplex assays reveal novel mechanisms of wound healing in diabetic foot ulcers[J]. Diabetes, 2020, 69(10): 2157-2169. DOI: 10.2337/db20-0188.
[52]	ⅡÁlvarez-Rodríguez,Castaño-TostadoE,García-GutiérrezDG,et al.Non-targeted metabolomic analysis reveals serum phospholipid alterations in patients with early stages of diabetic foot ulcer[J].Biomark Insights,2020,15:1177271920954828.DOI: 10.1177/1177271920954828.