Abstract: Objective To evaluate the biocompatibility of spongy PLGA-collagen membrane as a dermal scaffold. Methods Adult SD rats, cultured human keratinocytes and fibroblasts were employed in the study. The in vivo degradation process and the in vitro cytotoxicity and hemolytic response of the PLGA-collagen membrane, and the cell affinity of the PLGA membrane with the human cells were investigate. Results The PLGA-collagen membrane degraded completely in vivo at the 9th week with an acceptable speed, It was found that new tissues gradually grew into the pores of the matrix, and blood vessels could be the matrix at the 3rd week of implantation subcutaneously without obvious inflammatory reaction. The lixivium of the PLGA-collagen membrane did not induce apparent cytotoxicity and hemolytic response, and it could induce rapid adherence and proliferation of fibroblasts and keratinocytes on it. Conclusion The PLGA-collagen membrane posessed good biocompatibility cell affinity, with appropriate degradation speed, These properties seemed to meet the regairemeat of a tissue-engineering dermal scaffold.