Effects of thrombin peptides and thrombin on the release of TGF-β and VEGF from human fibroblasts
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摘要: 目的 探讨凝血酶受体激活肽TP508和α-凝血酶对体外培养的人成纤维细胞Lu13释放转化生长因子-β(TGF-β)和血管内皮生长因子(VEGF)的影响,以了解其促进创面愈合的部分机制 方法 在体外培养融合的Lu13中,加入不同浓度的凝血酶和TP508,置培养箱培养一定时间后,收集上清液离心,采用ELISA法测定TGF-β1、TGF-β2和VEGF的含量。 结果 α-凝血酶能显著促进Lu13释放TGF-β1;单纯TP508使Lu13释放TGF-β1,显著减少(
P <0.01),与小剂量凝血酶复合应用,对Lu13释放TGF-β1,有一定协同作用。经凝血酶处理后,Lu13释放TGF-β2也显著增高(P <0.05)。在较大剂量(100 μg/ml)时,TP508对Lu13释放TGF-β2呈轻度抑制作用(P >0.05)。经凝血酶处理后,VEGF释放量显著增加,TP508使Lu13释放VEGF减少。 结论 凝血酶可刺激Lu13释放TGF-β1,、TGF- β1,和VEGF,TP508抑制上述生长因子释放。TP508对TGF-β1,释放的抑制作用,可能有助于减少瘢痕形成,提高创面愈合质量。Abstract: Objective To explore the effect of thrombin peptide 508 (TP508) and thrombin on the release of TGF-β and VEGF from cultured human fibroblasts( Lu13 ) in vitro. Methods TP508 and thrombin in different concentrations were added to the confluent cultured Lu13 and incubated for 8 to 24 hours. Then the supernatant was obtained by centrifugation, and the TGF-β 1, TGF-β 2 and VEGF contents were measured by ELISA. Results TGF-β 1 release from Lu13 was markedly increased by thrombin, but decreased obviously by TP508 (P <0.05). A combinedation application of TP508 and thrombin showed a synergistic effect to the release of TGF-β1, from Lu13. The release of TGF-β2 was also increased(P <0.05) after thrombin treatment, while slightly decreased when incubated with 100ug/ml of TP508 (P > 0.05). VEGF release was also stimulated by thrombin and inhibited by TP508. Conclusion The release of TGF-β 1, TGF-β 2 and VEGF from Lu13 could be stimulated by thrombin, and inhibited by TP508, which might be beneficial to reducing scar formation and enhancing the healing quality of the wound.-
Key words:
- Trombin peptide /
- Fibroblast /
- Transforming growth factor-β /
- Endothelial growth factor
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