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Volume 39 Issue 8
Aug.  2023
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Article Navigation >  CHINESE JOURNAL OF BURNS AND WOUNDS  > 2023  >  39(8): 771-778
Tang LJ,Li XM,Zhang XW,et al.Effects of advanced platelet-rich fibrin on deep partial-thickness burn wounds in nude mice[J].Chin J Burns Wounds,2023,39(8):771-778.DOI: 10.3760/cma.j.cn501225-20220804-00334.
Citation: Tang LJ,Li XM,Zhang XW,et al.Effects of advanced platelet-rich fibrin on deep partial-thickness burn wounds in nude mice[J].Chin J Burns Wounds,2023,39(8):771-778.DOI: 10.3760/cma.j.cn501225-20220804-00334.
shu

Effects of advanced platelet-rich fibrin on deep partial-thickness burn wounds in nude mice

doi: 10.3760/cma.j.cn501225-20220804-00334
  • 1.

    Department of Burn Rehabilitation, Shanghai Yangzhi Rehabilitation Hospital (Shanghai Sunshine Rehabilitation Center), Tongji University, Shanghai 201613, China

  • 2.

    Department of Burns and Plastic Surgery of Subei People's Hospital, Clinical Medical College of Yangzhou University, Yangzhou 225001, China

Funds:

Science and Technology Development Plan of Traditional Chinese Medicine of Jiangsu Province of China YB2020086

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    Abstract
  •   Objective   To explore the effects of advanced platelet-rich fibrin (A-PRF) on deep partial-thickness burn wounds in nude mice and its mechanism.   Methods   The experimental study method was adopted. Forty healthy volunteers in Subei People's Hospital were recruited, including 32 females and 8 males, aged 60 to 72 years. Leukocyte platelet-rich fibrin (L-PRF) and A-PRF membranes were prepared after venous blood was extracted from them. The microstructure of two kinds of platelet-rich fibrin (PRF) membranes was observed by field emission scanning electron microscope. The number of samples was 3 in the following experiments. The L-PRF and A-PRF membranes were divided into L-PRF group and A-PRF group and cultured, and then the release concentrations of platelet-derived growth factor-AB (PDGF-AB) and vascular endothelial growth factor (VEGF) in culture supernatant were determined by enzyme-linked immunosorbent assay on culture day 1, 3, 7, and 14. Mice L929 fibroblasts (Fbs) were divided into L-PRF group and A-PRF group, and cultured with L-PRF or A-PRF conditioned medium, respectively. On culture day 1, 3, and 7, the cell proliferation activity was detected by thiazole blue method. The cell migration rate was detected and calculated at 24 h after scratching by scratch test. Thirty-six male BALB/c nude mice aged 6-8 weeks were selected to make a deep partial-thickness burn wound on one hind leg, and then divided into normal saline group, L-PRF group, and A-PRF group, according to the random number table, with 12 mice in each group. The wounds of nude mice in normal saline group were only washed by normal saline, while the wounds of nude mice in L-PRF group and A-PRF group were covered with the corresponding membranes in addition. The wounds of nude mice in the 3 groups were all bandaged and fixed with dressings. On treatment day 4, 7, and 14, the wound healing was observed and the wound healing rate was calculated. Masson staining was used to observe the new collagen in wound tissue, and immunohistochemical staining was used to detect the percentage of CD31 positive cells in the wound. Data were statistically analyzed with independent sample t test, analysis of variance for repeated measurement, analysis of variance for factorial design, one-way analysis of variance, and least significant difference test.   Results   L-PRF membrane's dense network structure was composed of coarse fibrin bundles, with scattered white blood cells and platelets with complete morphology. A-PRF membrane's loose network structure was composed of fine fibrin bundles, with scattered small amount of deformed white blood cells and platelets. On culture day 1, the release concentration of PDGF-AB in PRF culture supernatant in A-PRF group was significantly higher than that in L-PRF group ( t=5.73, P<0.05), while the release concentrations of VEGF in PRF culture supernatant in the two groups were similar ( P>0.05). On culture day 3, 7, and 14, the release concentrations of PDGF-AB and VEGF in PRF culture supernatant in A-PRF group were significantly higher than those in L-PRF group (with t values of 6.93, 7.45, 5.49, 6.97, 8.97, and 13.64, respectively, P<0.05). On culture day 3, 7, and 14, the release concentrations of PDGF-AB and VEGF in PRF culture supernatant in the two groups were all significantly higher than those in the previous time points within the group ( P<0.05). On culture day 1, 3, and 7, the proliferation activity of mice Fbs in A-PRF group was 0.293±0.034, 0.582±0.054, and 0.775±0.040, respectively, which were significantly stronger than 0.117±0.013, 0.390±0.036, and 0.581±0.037 in L-PRF group (with t values of 8.38, 5.14, and 6.16, respectively, P<0.05). At 24 h after scratching, the migration rate of mice Fbs in A-PRF group was (60.9±2.2)%, which was significantly higher than (39.1±2.3)% in L-PRF group ( t=11.74, P<0.05). On treatment day 4, the wound exudates of nude mice in L-PRF group and A-PRF group were less with no obvious signs of infection, while the wounds of nude mice in normal saline group showed more exudation. On treatment day 7, the wounds of nude mice in L-PRF group and A-PRF group were dry and crusted, while there was still a small amount of exudate in the wounds of nude mice in normal saline group. On treatment day 14, the wounds of nude mice in A-PRF group tended to heal; a small portion of wounds remained in nude mice in L-PRF group; the wound of nude mice was still covered with eschar in normal saline group. On treatment day 4, 7, and 14, the wound healing rate and percentage of CD31 positive cells of nude mice in L-PRF group were all significantly higher than those in normal saline group ( P<0.05); compared with those in normal saline group and L-PRF group, the wound healing rate of nude mice in A-PRF group was significantly increased ( P<0.05), the newborn collagen was orderly and evenly distributed, with no excessive deposition, and the percentage of CD31 positive cells was significantly increased ( P<0.05).   Conclusions   The stable fibrin network structure of A-PRF can maintain the sustained release of growth factors, accelerate cell proliferation, and promote cell migration, so as to shorten the healing time and improve the healing quality of deep partial-thickness burn wounds in nude mice.

     

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    • References(29)
  • [1]
    HettiaratchyS,DziewulskiP.ABC of burns: pathophysiology and types of burns[J].BMJ,2004,328(7453):1427-1429.DOI: 10.1136/bmj.328.7453.1427.
    [2]
    PeckMD.Epidemiology of burns throughout the world. Part I: distribution and risk factors[J].Burns,2011,37(7):1087-1100.DOI: 10.1016/j.burns.2011.06.005.
    [3]
    GragnaniA, TonarelliE, ChomiskiV, et al. Fibroblast growth factor in the treatment of burns: a systematic review[J].Burns,2022,48(1):104-110.DOI: 10.1016/j.burns.2021.04.006.
    [4]
    ShuWT, WangYN, ZhangX, et al. Functional hydrogel dressings for treatment of burn wounds[J]. Front Bioeng Biotechnol,2021,9:788461.DOI: 10.3389/fbioe.2021.788461.
    [5]
    HermansMH.Porcine xenografts vs. (cryopreserved) allografts in the management of partial thickness burns: is there a clinical difference?[J].Burns,2014,40(3):408-415.DOI: 10.1016/j.burns.2013.08.020.
    [6]
    WangY, BeekmanJ, HewJ, et al. Burn injury: challenges and advances in burn wound healing, infection, pain and scarring[J]. Adv Drug Deliv Rev,2018,123:3-17.DOI: 10.1016/j.addr.2017.09.018.
    [7]
    HexterAT,Sanghani-KeraiA,HeidariN,et al.Mesenchymal stromal cells and platelet-rich plasma promote tendon allograft healing in ovine anterior cruciate ligament reconstruction[J].Knee Surg Sports Traumatol Arthrosc,2021,29(11):3678-3688.DOI: 10.1007/s00167-020-06392-9.
    [8]
    KarimiK, RockwellH. The benefits of platelet-rich fibrin[J]. Facial Plast Surg Clin North Am,2019,27(3):331-340.DOI: 10.1016/j.fsc.2019.03.005.
    [9]
    NakanishiY, MatsushitaT, NagaiK, et al. Fibrin clot and leukocyte-rich platelet-rich fibrin show similar release kinetics and amount of growth factors: a pilot study[J]. J Orthop Surg Res,2023,18(1):238.DOI: 10.1186/s13018-023-03709-5.
    [10]
    WangPH,HuangBS,HorngHC,et al.Wound healing[J].J Chin Med Assoc,2018,81(2):94-101.DOI: 10.1016/j.jcma.2017.11.002.
    [11]
    KwonSH,BarreraJA,NoishikiC,et al.Current and emerging topical scar mitigation therapies for craniofacial burn wound healing[J].Front Physiol,2020,11:916.DOI: 10.3389/fphys.2020.00916.
    [12]
    ZubairM, AhmadJ. Role of growth factors and cytokines in diabetic foot ulcer healing: a detailed review[J]. Rev Endocr Metab Disord,2019,20(2):207-217.DOI: 10.1007/s11154-019-09492-1.
    [13]
    MironRJ,DhamA,DhamU,et al.The effect of age, gender, and time between blood draw and start of centrifugation on the size outcomes of platelet-rich fibrin (PRF) membranes[J].Clin Oral Investig,2019,23(5):2179-2185.DOI: 10.1007/s00784-018-2673-x.
    [14]
    BoltonL. Platelet-rich plasma: optimal use in surgical wounds[J]. Wounds,2021,33(8):219-221. DOI: 10.25270/wnds/2021.219221.
    [15]
    Vidán-EstévezJ, Sánchez-HerráezS, Escalante-BarrigónF, et al. Healing of chronic wounds with platelet-derived growth factors from single donor platelet-rich plasma following one freeze-thaw cycle. A cross-sectional study[J]. J Clin Med,2021,10(24):5762.DOI: 10.3390/jcm10245762.
    [16]
    OlivaN,AlmquistBD.Spatiotemporal delivery of bioactive molecules for wound healing using stimuli-responsive biomaterials[J].Adv Drug Deliv Rev,2020,161-162:22-41.DOI: 10.1016/j.addr.2020.07.021.
    [17]
    Castillo-HenríquezL, Castro-AlpízarJ, Lopretti-CorreaM, et al. Exploration of bioengineered scaffolds composed of thermo-responsive polymers for drug delivery in wound healing[J]. Int J Mol Sci,2021,22(3):1408.DOI: 10.3390/ijms22031408.
    [18]
    LiuMQ, WeiXR, ZhengZJ, et al. Recent advances in nano-drug delivery systems for the treatment of diabetic wound healing[J]. Int J Nanomedicine,2023,18:1537-1560.DOI: 10.2147/IJN.S395438.
    [19]
    ShahSA, SohailM, KhanS, et al. Biopolymer-based biomaterials for accelerated diabetic wound healing: a critical review[J]. Int J Biol Macromol,2019,139:975-993.DOI: 10.1016/j.ijbiomac.2019.08.007.
    [20]
    García-SánchezJM, Mirabet LisV, Ruiz-VallsA, et al. Platelet rich plasma and plasma rich in growth factors for split-thickness skin graft donor site treatment in the burn patient setting: a randomized clinical trial[J].Burns,2022,48(7):1662-1670.DOI: 10.1016/j.burns.2021.10.001.
    [21]
    BernatchezSF, BichelJ. The science of skin: measuring damage and assessing risk[J]. Adv Wound Care (New Rochelle),2023,12(4):187-204.DOI: 10.1089/wound.2022.0021.
    [22]
    ShenSH,WangFY,FernandezA,et al.Role of platelet-derived growth factor in type Ⅱ diabetes mellitus and its complications[J].Diab Vasc Dis Res,2020,17(7):1479164120942119.DOI: 10.1177/1479164120942119.
    [23]
    CuiHS, ChoYS, JooSY, et al. Wound healing potential of low temperature plasma in human primary epidermal keratinocytes[J]. Tissue Eng Regen Med,2019,16(6):585-593.DOI: 10.1007/s13770-019-00215-w.
    [24]
    GaroufaliaZ, PapadopetrakiA, KaratzaE, et al. Insulin-like growth factor-I and wound healing, a potential answer to non-healing wounds: a systematic review of the literature and future perspectives[J]. Biomed Rep,2021,15(2):66.DOI: 10.3892/br.2021.1442.
    [25]
    FangZ,YangX,WuG,et al.The use of autologous platelet-rich plasma gel increases wound healing and reduces scar development in split-thickness skin graft donor sites[J].J Plast Surg Hand Surg,2019,53(6):356-360.DOI: 10.1080/2000656X.2019.1635489.
    [26]
    OgaiK,ShibataK,TakahashiN,et al.Amplicon-based skin microbiome profiles collected by tape stripping with different adhesive film dressings: a comparative study[J].BMC Microbiol,2021,21(1):54.DOI: 10.1186/s12866-021-02122-4.
    [27]
    GuptaA, ChannaveeraC, SethiS, et al. Efficacy of intralesional platelet-rich plasma in diabetic foot ulcer[J]. J Am Podiatr Med Assoc,2021,111(3):Article_7.DOI: 10.7547/19-149.
    [28]
    洪武,李少峰,林钰梅.富血小板纤维蛋白联合网状中厚皮植皮在手背深度烧伤创面中的应用[J].中国医药科学,2022,12(21):193-196.DOI: 10.3969/j.issn.2095-0616.2022.21.049.
    [29]
    刘中波,周大鹏,左娜,等.富血小板纤维蛋白联合薄中厚皮片治疗小面积深度皮肤损伤的临床研究[J].中国临床实用医学,2023,14(2):46-49.DOI: 10.3760/cma.j.cn115570-20230107-00011.
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