Targeted energy metabolomics study on wound tissue of diabetic rats

Wang Xiaoyang; Hu Yujie; Wang Xiaochuan; Zhao Jie; Zhang Jixun; Jiang Duyin

doi:10.3760/cma.j.cn501225-20241014-00385

Volume 41 Issue 2

Feb. 2025

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Article Navigation > CHINESE JOURNAL OF BURNS AND WOUNDS > 2025 > 41(2): 137-144

Wang XY,Hu YJ,Wang XC,et al.Targeted energy metabolomics study on wound tissue of diabetic rats[J].Chin J Burns Wounds,2025,41(2):137-144.DOI: 10.3760/cma.j.cn501225-20241014-00385.

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Wang XY,Hu YJ,Wang XC,et al.Targeted energy metabolomics study on wound tissue of diabetic rats[J].Chin J Burns Wounds,2025,41(2):137-144.DOI: 10.3760/cma.j.cn501225-20241014-00385.

Citation:

Wang XY,Hu YJ,Wang XC,et al.Targeted energy metabolomics study on wound tissue of diabetic rats[J].Chin J Burns Wounds,2025,41(2):137-144.DOI: 10.3760/cma.j.cn501225-20241014-00385.

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Targeted energy metabolomics study on wound tissue of diabetic rats

doi: 10.3760/cma.j.cn501225-20241014-00385

1.
Emergency Medicine Center, the Second Hospital of Shandong University, Jinan 250033, China
2.
Department of Plastic and Burns Surgery, the Second Hospital of Shandong University, Jinan 250033, China

Funds:

General Program of National Natural Science Foundation of China 81873934

The Youth Science Fund Project of National Natural Science Foundation of China 82202454

Growth Factor Rejuvenation Plan of Wang Zhengguo Foundation for Traumatic Medicine SZYZ-TR-09

Science and Technology Development Project of Shandong Province of China 2015GSF118041

The Youth Fund Project of Natural Science Foundation of Shandong Province of China ZR2020QH168

Jinan Science and Technology Plan 202225065

More Information

Corresponding author: Jiang Duyin, Email: jdybs2@vip.163.com
Received Date: 2024-10-14

Abstract

Abstract

Objective To explore the change of energy metabolism in wound tissue of diabetic rats. Methods This study was an experimental study. Six 8-week-old male Sprague-Dawley rats were divided into diabetic group inflicted with a diabetic full-thickness skin defect wound and control group only inflicted with a full-thickness skin defect wound according to the random number table method, with 3 rats in each group. The wound healing rates of rats in the two groups were calculated at 14 d after operation. The wound tissue of two groups of rats was collected at 14 d after operation, the targeted energy metabolomics detection was carried out by chromatography-mass spectrometry analysis, and the differential energy metabolites in the wound tissue were screened with significant change in the expression between the two groups of rats. The Kyoto encyclopedia of genes and genomes enrichment analysis was performed on the differential energy metabolites. Results At 14 d after operation, the wound healing rate of rats in diabetic group was (68.3±2.8)%, which was significantly lower than (98.1±1.2)% in control group (t=16.92, P<0.05). At 14 d after operation, compared with those in control group, the expressions of cis-aconitic acid, nicotinamide adenine dinucleotide phosphate, and 5'-guanosine diphosphate were significantly increased in wound tissue of rats in diabetic group (with t values of 4.74, 3.09, and 3.99, respectively, P<0.05), the expressions of L-malic acid and lactic acid were significantly decreased (with t values of 3.45 and 12.20, respectively, P<0.05), and there were no statistically significant differences in the expression levels of the other 21 energy metabolites in wound tissue of rats in the two groups (P>0.05). The five differential energy metabolites were enriched mainly in the glucagon, Rap1, Ras, hypoxia-inducible factor 1 signaling pathways as well as the tricarboxylic acid cycle, pyruvate metabolism, mitochondrial autophagy,and endocytosis pathways. Conclusions The expressions of lactic acid and L-malic acid in wound tissue of diabetic rats decreased, while the expressions of cis-aconitic acid, nicotinamide adenine dinucleotide phosphate, and 5'-guanosine diphosphate increased. The differential energy metabolites were mainly enriched in oxidative stress, energy regulation, inflammatory reaction, and other related signaling pathways or pathways.
- Wounds and injuries,
- Diabetes mellitus,
- Energy metabolism,
- Citric acid cycle,
- Lactic acid,
- L-malic acid

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References(41)

References

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