Explorative study of the immobilizing effect of full-thickness skin subcutaneous grafting on allogeneic full-thickness skin graft in rats
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摘要:
目的 探讨大鼠全层皮肤皮下移植异体全厚皮的制动效果。 方法 采用实验研究方法。取近交系雄性6~8周龄Brown-Norway大鼠和Lewis大鼠各10只，分别作为供体和受体。将受体大鼠项背部全层皮肤皮下游离2.2 cm×2.2 cm区域后，皮下移植取自供体大鼠腹部的2.0 cm×2.0 cm大小全厚皮，供区拉拢缝合。移植术后5~6 d剪除受体大鼠移植的异体皮片上方的自体皮，剪除后7 d拆线。移植术后2个月内观察供受体大鼠进食、活动、存活情况，受体大鼠撕咬、抓挠创面情况，受体大鼠剪除自体皮后创面情况，移植的异体皮片成活及毛发生长情况。 结果 移植术后2个月内，供受体大鼠均正常进食，可自由活动，无大鼠发生非正常死亡；未发生受体大鼠撕咬、搔抓创面情况。受体大鼠剪除自体皮后创面有少量渗出，并有部分表皮脱痂现象。移植的异体皮片均未发生感染或坏死，全部成活，新毛发顺利长出。 结论 大鼠全层皮肤皮下移植异体全厚皮的制动方法操作简单省时，术后无须换药，加压固定可靠，皮片成活率高，可推广应用于动物皮片移植模型。 Abstract:Objective To investigate the immobilizing effect of full-thickness skin subcutaneous grafting on allogeneic full-thickness skin graft in rats. Methods The experimental research method was used. The inbred male Brown-Norway rats (n=10) and Lewis rats (n=10) were used as donors and recipients respectively. After subcutaneously full-thickness separation of a 2.2 cm×2.2 cm area on the nape of the recipient rat, a full-thickness skin of 2.0 cm×2.0 cm taken from the abdomen of the donor rat was subcutaneously grafted, and the donor site was pulled together and sutured. The autologous skin over the allograft in the recipient rat was excised 5-6 d after grafting, and the stitches were removed 7 d after excision. Within 2 months after grafting, the feeding, activity, and survival of the donor and recipient rats, behavior of tearing and scratching the wounds of the recipient rats, the wound condition after autologous skin excision in recipient rats, and the survival and hair growth of the grafted allogeneic skin were observed. Results Within 2 months after grafting, the donor and recipient rats all ate normally and could move freely with no abnormal death. No tearing or scratching of the wounds occurred in recipient rats. There was a small amount of exudation and partial epidermal desquamation after autologous skin excision in recipient rats. All transplanted allografts survived, which were free of infection and necrosis, with new hairs growing out smoothly. Conclusions The immobilizing method of full-thickness skin subcutaneous grafting of allogeneic full-thickness skin graft in rats is simple and time-saving without postoperative dressing change, with reliable pressure fixation and high survival rate of skin grafts, which can be promoted for animal skin grafting models. -
Key words:
- Models, animal /
- Skin transplantation /
- Wound healing
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1 大鼠全层皮肤皮下移植异体全厚皮的操作。1A.Brown-Norway大鼠腹部全厚皮设计；1B.将Brown-Norway大鼠全厚皮从Lewis大鼠项背部切口植入全层皮肤下；1C.放置引流皮条并缝合皮片与切口
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[1] PeiJM,LiY,ChenC,et al.Inhibition of lymphatic drainage with a self-designed surgical approach prolongs the vascularized skin allograft survival in rats[J].Ann Plast Surg,2018,80(1):76-82.DOI: 10.1097/SAP.0000000000001210. [2] ZhaiQY,ZhouF,IbrahimMM,et al.An immune-competent rat split thickness skin graft model: useful tools to develop new therapies to improve skin graft survival[J].Am J Transl Res,2018,10(6):1600-1610. [3] 施彦,涂龙翔,邓琴,等.经外源性血管内皮生长因子处理的大鼠表皮干细胞对深Ⅱ度烧伤大鼠创面愈合的影响及机制[J].中华烧伤杂志,2020,36(3):195-203.DOI: 10.3760/cma.j.cn501120-20191125-00441. [4] RandallP.Extending the use of the tied-on dressing[J].Plast Reconstr Surg,1962,29(6):708-709.DOI: 10.1097/00006534-196206000-00011. [5] 周锦烽,徐艳,郝超.自制约束材料在大鼠植皮模型中的应用[J].中华烧伤杂志,2019,35(8):628-629.DOI: 10.3760/cma.j.issn.1009-2587.2019.08.018. -
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