瘢痕疙瘩患者瘢痕疙瘩与其周围正常皮肤水屏障功能的差异及其相关机制

于磊; 杨雅婷; 刘伟

doi:10.3760/cma.j.cn501120-20210427-00156

瘢痕疙瘩患者瘢痕疙瘩与其周围正常皮肤水屏障功能的差异及其相关机制

doi: 10.3760/cma.j.cn501120-20210427-00156

于磊¹,
杨雅婷²,
刘伟^2, ,

1.
潍坊医学院整形外科研究所，潍坊　　261042
2.
上海交通大学医学院附属第九人民医院整复外科，上海市组织工程研究重点实验室，上海　　200011

基金项目:

国家自然科学基金面上项目 81671921

详细信息

通讯作者:
刘伟，Email：liuwei_md@126.com

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出版历程
- 收稿日期: 2021-04-27

Differences of water barrier function between keloid and its surrounding normal skin in patients with keloids and its related mechanism

Yu Lei¹,
Yang Yating²,
Liu Wei^{2
, ,}

1.
Plastic Surgery Research Institute, Weifang Medical University, Weifang 261042, China
2.
Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai Tissue Engineering Key Laboratory, Shanghai 200011, China

Funds:

General Program of National Natural Science Foundation of China 81671921

More Information

Corresponding author: Liu Wei, Email: liuwei_md@126.com

摘要

摘要: 目的比较瘢痕疙瘩患者的瘢痕疙瘩与其周围正常皮肤的皮肤水屏障功能的差异并初步探索相关机制。方法采用横断面观察性研究方法。2020年10月—2021年3月，上海交通大学医学院附属第九人民医院接诊30例符合入选标准的瘢痕疙瘩患者，其中男12例、女18例，年龄20~48岁。接诊当日采用多功能皮肤测试仪测定30例患者瘢痕疙瘩皮肤及其周围正常皮肤的经皮水分丢失（TEWL）。对5例患者瘢痕疙瘩修复术后的瘢痕疙瘩皮肤及其周围正常皮肤行苏木精-伊红染色观测表皮厚度，取其中3例患者标本采用免疫组织化学法检测瘢痕疙瘩皮肤及正常皮肤细胞角蛋白10、内披蛋白和聚丝蛋白的表达。对数据行配对样本 t检验或独立样本 t检验。结果接诊当日，30例患者瘢痕疙瘩皮肤TEWL为9.0（6.9，13.4）g·m ^-2·h ^-1，正常皮肤的TEWL为8.1（6.4，18.1）g·m ^-2·h ^-1，二者比较差异无统计学意义（ t=0.44， P>0.05）。瘢痕疙瘩修复术后，5例患者瘢痕疙瘩皮肤表皮厚度为（194±44）μm，明显厚于正常皮肤的（44±11）μm（ t=6.88， P<0.01）；且瘢痕疙瘩区域均存在角质形成细胞（KC）数量增多、正常皮肤的表皮脊结构缺乏、表皮明显增厚等现象。瘢痕疙瘩修复术后，3例患者瘢痕疙瘩皮肤细胞角蛋白10表达明显低于正常皮肤（ t=8.50， P<0.01），而瘢痕疙瘩皮肤表皮的内披蛋白和聚丝蛋白表达均与正常皮肤相近（ t值分别为0.07、0.96， P>0.05）。结论瘢痕疙瘩患者瘢痕疙瘩皮肤组织中存在KC增多、表皮增厚的现象，但瘢痕疙瘩区域的水屏障功能与周围正常皮肤组织相近，TEWL可能并不是影响瘢痕疙瘩持续发展的主要机制。
- 瘢痕疙瘩 /
- 表皮 /
- 皮肤水屏障功能 /
- 角质形成细胞 /
- 经皮水分丢失
Abstract: Objective To compare the differences of water barrier function between keloids and its surrounding normal skin in patients with keloids, and to explore the primary mechanism. Methods A cross-sectional observational study was conducted. From October 2020 to March 2021, 30 patients with keloids who met the inclusion criteria visited Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, including 18 females and 12 males, aged 20-48 years. The transepidermal water loss (TEWL) of their keloids and the surrounding normal skin of the 30 patients were measured by multi probe adapter on the reception day. The keloid tissues and normal skin of 5 patients after keloid repair surgery were processed for hematoxylin-eosin staining to measure the thickness of epidermis. Immunohistochemistry was performed on samples from 3 of those 5 patients to detect the expressions of cytokeratin-10, involucrin, and filaggrin in keloids and normal skin. Data were statistically analyzed with paired sample ttest and independent sample t test. Results On the reception day, the TEWL of keloids of 30 patients was 9.0 (6.9, 13.4) g·m ^-2·h ^-1 and the TEWL of the normal skin was 8.1 (6.4, 18.1) g·m ^-2·h ^-1, between which the difference was not statistically significant ( t=0.44, P>0.05). After keloid repair surgery, the thickness of epidermis in the keloids of 5 patients was (194±44) μm, which was significantly thicker than that of the normal skin (44±11) μm, ( t=6.88, P<0.01). Furthermore, increased keratinocytes, lack of normal epidermal ridge structures, and thickened stratum corneum were observed in the keloid area. After keloid repair surgery, the expression level of cytokeratin-10 in keloids was significantly lower than that in normal skin of 3 patients ( t=8.50, P<0.01), but there were no statistically significant differences in the expression levels of involucrin or filaggrin between keloids and normal skin (with t values of 0.07 and 0.96, respectively, P>0.05). Conclusions Keloid tissue from patients with keloids displays increased number of keratinocytes and thickened epidermis. But the water barrier function in keloid area is similar to the surrounding normal skin, suggesting that TEWL may not be the main mechanism lead to the persistent development of keloids.
- Keloid /
- Epidermis /
- Skin water barrier function /
- Keratinocyte /
- Transepidermal water loss
于磊：实施研究、采集数据、分析/解释数据；杨雅婷：起草文章，对文章的知识性内容作批判性审阅、统计分析；刘伟：酝酿和设计实验、分析/解释数据、对文章的知识性内容作批判性审阅、统计分析、获取研究经费、论文修改和指导

所有作者均声明不存在利益冲突

该研究旨在探讨血红素加氧酶1在烧伤早期急性肾损伤中的作用及其相关机制。采用100 ℃水浴建立经典的大鼠烧伤模型，伤后即刻腹腔注射血红素加氧酶1。采用基于结构变化和功能的组织病理学和血生物化学评估早期急性肾损伤进展，ELISA、免疫染色、实时荧光定量PCR和蛋白质印迹法检测血红素加氧酶1、氧化应激、促炎症介质和Toll样受体4（TLR4）相关信号通路。使用TLR4抑制剂环己烯衍生物TAK242和诱导剂LPS检测血红素加氧酶1在烧伤大鼠肾脏炎症及TLR4信号通路中的作用。研究表明，氯化高铁血红素诱导的血红素加氧酶1通过减少肾氧化应激和释放促炎症介质，下调TLR4的表达及下游核因子κB抑制剂（IκB）激酶α/β、IκBα和核因子κB p65的磷酸化，改善烧伤大鼠的肾损伤和功能障碍；TAK242的作用与血红素加氧酶1相似但较弱；LPS抑制了血红素加氧酶1的抗炎及TLR4信号的调节作用。结果证实血红素加氧酶1通过TLR4/核因子κB信号途径保护肾脏。

吴学洁,编译自《Burns》, 2021： S0305-4179(21)00094-2;申传安,审校

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1 1例瘢痕疙瘩患者瘢痕疙瘩皮肤和正常皮肤的组织形态学观察苏木精-伊红×100，图中标尺为200 µm。1A.正常皮肤；1B.瘢痕疙瘩皮肤，较图1A表皮增厚，细胞层数增多

下载: 全尺寸图片幻灯片

2 免疫组织化学法检测瘢痕疙瘩患者瘢痕疙瘩皮肤与正常皮肤细胞角蛋白10、内披蛋白、聚丝蛋白的表达情况二氨基联苯胺-苏木精×100，图中标尺为100 μm。2A、2B、2C.分别为正常皮肤细胞角蛋白10、内披蛋白、聚丝蛋白阳性表达；2D、2E、2F.分别为瘢痕疙瘩皮肤细胞角蛋白10、内披蛋白、聚丝蛋白阳性表达，其中图2D染色明显较图2A浅，图2E的棕色区域较图2B分布更广，图2F与图2C阳性表达相近

注：细胞角蛋白10、内披蛋白、聚丝蛋白阳性染色均为棕色

下载: 全尺寸图片幻灯片