微小RNA工程化外泌体在糖尿病创面中的作用研究进展

简喜超; 邓呈亮

doi:10.3760/cma.j.cn501225-20230721-00011

微小RNA工程化外泌体在糖尿病创面中的作用研究进展

doi: 10.3760/cma.j.cn501225-20230721-00011

简喜超,
邓呈亮^,

遵义医科大学附属医院烧伤整形外科，遵义　　563003

基金项目:

贵州省科技计划项目（黔科合基础-ZK［2021］重点011、黔科合支撑［2021］一般079、黔科合基础［2020］1Y331） ZK2021-011, 2021-079, 2020-1Y331

省部共建协同创新中心项目 2020-39

详细信息

通讯作者:
邓呈亮，Email：cheliadeng@sina.com

计量
- 文章访问数: 175
- HTML全文浏览量: 106
- PDF下载量: 33
- 被引次数: 0
出版历程
- 收稿日期: 2023-07-21
- 网络出版日期: 2024-02-26

Research advances on the role of microRNA engineered exosomes in diabetic wounds

Jian Xichao,
Deng Chengliang^,

Department of Burns and Plastic Surgery, Affiliated Hospital of Zunyi Medical University, Zunyi 563003, China

Funds:

Science and Technology Plan Project of Guizhou Province of China ZK2021-011, 2021-079, 2020-1Y331

Collaborative Innovation Center of Chinese Ministry of Education 2020-39

More Information

Corresponding author: Deng Chengliang, Email: cheliadeng@sina.com

摘要

摘要: 糖尿病创面是糖尿病患者常见的并发症之一，治疗较为困难。目前，糖尿病创面的治疗方法包括清创、功能性敷料覆盖、负压治疗、骨水泥填充、皮肤移植等。微小RNA（miRNA）工程化外泌体具有减轻炎症、刺激血管生成、促进胶原沉积和再上皮化等功能，在糖尿病创面修复中展现出巨大潜力，相关研究正在积极开展。该文对糖尿病创面的病理生理特征、miRNA与外泌体的特性、外泌体负载miRNA的工程化方法和miRNA工程化外泌体促进糖尿病创面愈合的机制进行综述，以期为miRNA工程化外泌体在糖尿病创面中的临床应用提供参考依据。
- 外泌体 /
- 微RNAs /
- 糖尿病 /
- 创面修复
Abstract: Diabetic wounds are a common complication in patients with diabetes, which is difficult to treat. Current treatment methods for diabetic wounds include debridement, functional dressing coverage, negative pressure therapy, bone cement filling, and skin grafting, etc. MicroRNA (miRNA) engineered exosomes have shown promising potential in diabetic wound repair due to the ability to alleviate inflammation, stimulate angiogenesis, and promote collagen deposition and re-epithelialization. Related researches are being actively carred out. This paper reviews the pathophysiological characteristics of diabetic wounds, the characteristics of miRNA and exosomes, the engineering methods for exosomes loaded with miRNA, and the mechanism of miRNA engineered exosomes in promoting healing of diabetic wounds, aiming to provide a reference basis for the future clinical application of miRNA engineered exosomes in diabetic wounds.
- Exosomes /
- MicroRNAs /
- Diabetes mellitus /
- Wound repair
所有作者均声明不存在利益冲突

HTML全文

参考文献(43)

[1]	SunH,SaeediP,KarurangaS,et al.IDF Diabetes Atlas: global, regional and country-level diabetes prevalence estimates for 2021 and projections for 2045[J].Diabetes Res Clin Pract,2022,183:109119.DOI: 10.1016/j.diabres.2021.109119.
[2]	ArmstrongDG,BoultonA,BusSA.Diabetic foot ulcers and their recurrence[J].N Engl J Med,2017,376(24):2367-2375.DOI: 10.1056/NEJMra1615439.
[3]	中国医疗保健国际交流促进会糖尿病足病分会,国际血管联盟中国分部糖尿病足病专家委员会.中国糖尿病足诊治指南[J].中国临床医生杂志,2020,48(1):19-27.DOI: 10.3969/j.issn.2095-8552.2020.01.007.
[4]	海峡两岸医药卫生交流协会烧创伤暨组织修复专委会.负压伤口疗法在糖尿病足创面治疗中的应用全国专家共识(2021版)[J].中华烧伤杂志,2021,37(6):508-518.DOI: 10.3760/cma.j.cn501120-20210107-00010.
[5]	WeiP, ZhongC, YangX, et al. Exosomes derived from human amniotic epithelial cells accelerate diabetic wound healing via PI3K-AKT-mTOR-mediated promotion in angiogenesis and fibroblast function[J/OL]. Burns Trauma,2020,8: tkaa020[2023-07-21]. https://pubmed.ncbi.nlm.nih.gov/32923490/. DOI: 10.1093/burnst/tkaa020.
[6]	SoheilifarMH,Masoudi-KhoramN.Wound dressings incorporating microRNAs: innovative therapy for diabetic wound treatment[J].Iran J Basic Med Sci,2022,25(9):1042-1044.DOI: 10.22038/IJBMS.2022.67236.14739.
[7]	MouraJ,MadureiraP,LealEC,et al.Immune aging in diabetes and its implications in wound healing[J].Clin Immunol,2019,200:43-54.DOI: 10.1016/j.clim.2019.02.002.
[8]	ChenT, SongP, HeM, et al. Sphingosine-1-phosphate derived from PRP-Exos promotes angiogenesis in diabetic wound healing via the S1PR1/AKT/FN1 signalling pathway[J/OL]. Burns Trauma, 2023, 11: tkad003[2023-07-21]. https://pubmed.ncbi.nlm.nih.gov/37251708/. DOI: 10.1093/burnst/tkad003.
[9]	CatrinaSB,ZhengX.Disturbed hypoxic responses as a pathogenic mechanism of diabetic foot ulcers[J].Diabetes Metab Res Rev,2016,32 Suppl 1:179-185.DOI: 10.1002/dmrr.2742.
[10]	PetkovicM,SørensenAE,LealEC,et al.Mechanistic actions of microRNAs in diabetic wound healing[J].Cells,2020,9(10):2228.DOI: 10.3390/cells9102228.
[11]	BartelDP.MicroRNAs: genomics, biogenesis, mechanism, and function[J].Cell,2004,116(2):281-297.DOI: 10.1016/s0092-8674(04)00045-5.
[12]	PegtelDM,GouldSJ.Exosomes[J].Annu Rev Biochem,2019,88:487-514.DOI: 10.1146/annurev-biochem-013118-111902.
[13]	GualdiG,CostantiniE,RealeM,et al.Wound repair and extremely low frequency-electromagnetic field: insight from in vitro study and potential clinical application[J].Int J Mol Sci,2021,22(9):5037.DOI: 10.3390/ijms22095037.
[14]	DesmetCM,PréatV,GallezB.Nanomedicines and gene therapy for the delivery of growth factors to improve perfusion and oxygenation in wound healing[J].Adv Drug Deliv Rev,2018,129:262-284.DOI: 10.1016/j.addr.2018.02.001.
[15]	JonesJI,NguyenTT,PengZ,et al.Targeting MMP-9 in diabetic foot ulcers[J].Pharmaceuticals (Basel),2019,12(2):79.DOI: 10.3390/ph12020079.
[16]	FalangaV.Wound healing and its impairment in the diabetic foot[J].Lancet,2005,366(9498):1736-1743.DOI: 10.1016/S0140-6736(05)67700-8.
[17]	BartelDP.MicroRNAs: target recognition and regulatory functions[J].Cell,2009,136(2):215-233.DOI: 10.1016/j.cell.2009.01.002.
[18]	LewisBP,BurgeCB,BartelDP.Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets[J].Cell,2005,120(1):15-20.DOI: 10.1016/j.cell.2004.12.035.
[19]	ZhangY,LiuY,LiuH,et al.Exosomes: biogenesis, biologic function and clinical potential[J].Cell Biosci,2019,9:19.DOI: 10.1186/s13578-019-0282-2.
[20]	MartinsTS,VazM,HenriquesAG.A review on comparative studies addressing exosome isolation methods from body fluids[J].Anal Bioanal Chem,2023,415(7):1239-1263.DOI: 10.1007/s00216-022-04174-5.
[21]	ManzariMT,ShamayY,KiguchiH,et al.Targeted drug delivery strategies for precision medicines[J].Nat Rev Mater,2021,6(4):351-370.DOI: 10.1038/s41578-020-00269-6.
[22]	ChoiH,ChoiY,YimHY,et al.Biodistribution of exosomes and engineering strategies for targeted delivery of therapeutic exosomes[J].Tissue Eng Regen Med,2021,18(4):499-511.DOI: 10.1007/s13770-021-00361-0.
[23]	LvQ,DengJ,ChenY,et al.Engineered human adipose stem-cell-derived exosomes loaded with miR-21-5p to promote diabetic cutaneous wound healing[J].Mol Pharm,2020,17(5):1723-1733.DOI: 10.1021/acs.molpharmaceut.0c00177.
[24]	EsmaeiliA,HosseiniS,Baghaban EslaminejadM.Engineered-extracellular vesicles as an optimistic tool for microRNA delivery for osteoarthritis treatment[J].Cell Mol Life Sci,2021,78(1):79-91.DOI: 10.1007/s00018-020-03585-w.
[25]	PomattoM,BussolatiB,D'AnticoS,et al.Improved loading of plasma-derived extracellular vesicles to encapsulate antitumor miRNAs[J].Mol Ther Methods Clin Dev,2019,13:133-144.DOI: 10.1016/j.omtm.2019.01.001.
[26]	ZhangD,LeeH,ZhuZ,et al.Enrichment of selective miRNAs in exosomes and delivery of exosomal miRNAs in vitro and in vivo[J].Am J Physiol Lung Cell Mol Physiol,2017,312(1):L110-L121.DOI: 10.1152/ajplung.00423.2016.
[27]	AkersJC,RamakrishnanV,YangI,et al.Optimizing preservation of extracellular vesicular miRNAs derived from clinical cerebrospinal fluid[J].Cancer Biomark,2016,17(2):125-132.DOI: 10.3233/CBM-160609.
[28]	GondaliyaP,SayyedAA,BhatP,et al.Mesenchymal stem cell-derived exosomes loaded with miR-155 inhibitor ameliorate diabetic wound healing[J].Mol Pharm,2022,19(5):1294-1308.DOI: 10.1021/acs.molpharmaceut.1c00669.
[29]	MondalJ,PillarisettiS,JunnuthulaV,et al.Hybrid exosomes, exosome-like nanovesicles and engineered exosomes for therapeutic applications[J].J Control Release,2023,353:1127-1149.DOI: 10.1016/j.jconrel.2022.12.027.
[30]	YaoY,JiangY,SongJ,et al.Exosomes as potential functional nanomaterials for tissue engineering[J].Adv Healthc Mater,2023,12(16):e2201989.DOI: 10.1002/adhm.202201989.
[31]	LiQ,HuW,HuangQ,et al.MiR146a-loaded engineered exosomes released from silk fibroin patch promote diabetic wound healing by targeting IRAK1[J].Signal Transduct Target Ther,2023,8(1):62.DOI: 10.1038/s41392-022-01263-w.
[32]	YangZ,ShiJ,XieJ,et al.Large-scale generation of functional mRNA-encapsulating exosomes via cellular nanoporation[J].Nat Biomed Eng,2020,4(1):69-83.DOI: 10.1038/s41551-019-0485-1.
[33]	ChaJM,ShinEK,SungJH,et al.Efficient scalable production of therapeutic microvesicles derived from human mesenchymal stem cells[J].Sci Rep,2018,8(1):1171.DOI: 10.1038/s41598-018-19211-6.
[34]	BiH,FeiQ,LiR,et al.Disruption of miRNA sequences by TALENs and CRISPR/Cas9 induces varied lengths of miRNA production[J].Plant Biotechnol J,2020,18(7):1526-1536.DOI: 10.1111/pbi.13315.
[35]	Da SilvaJ,LealEC,CarvalhoE,et al.Innovative functional biomaterials as therapeutic wound dressings for chronic diabetic foot ulcers[J].Int J Mol Sci,2023,24(12):9900.DOI: 10.3390/ijms24129900.
[36]	TaoSC,GuoSC,LiM,et al.Chitosan wound dressings incorporating exosomes derived from microRNA-126-overexpressing synovium mesenchymal stem cells provide sustained release of exosomes and heal full-thickness skin defects in a diabetic rat model[J].Stem Cells Transl Med,2017,6(3):736-747.DOI: 10.5966/sctm.2016-0275.
[37]	SathyarajWV,PrabakaranL,BhoopathyJ,et al.Therapeutic efficacy of polymeric biomaterials in treating diabetic wounds-an upcoming wound healing technology[J].Polymers (Basel),2023,15(5):1205.DOI: 10.3390/polym15051205.
[38]	ZhaoX,FuL,ZouH,et al.Optogenetic engineered umbilical cord MSC-derived exosomes for remodeling of the immune microenvironment in diabetic wounds and the promotion of tissue repair[J].J Nanobiotechnology,2023,21(1):176.DOI: 10.1186/s12951-023-01886-3.
[39]	GeL,WangK,LinH,et al.Engineered exosomes derived from miR-132-overexpresssing adipose stem cells promoted diabetic wound healing and skin reconstruction[J].Front Bioeng Biotechnol,2023,11:1129538.DOI: 10.3389/fbioe.2023.1129538.
[40]	ManuelliV,PecorariC,FilomeniG,et al.Regulation of redox signaling in HIF-1-dependent tumor angiogenesis[J].FEBS J,2022,289(18):5413-5425.DOI: 10.1111/febs.16110.
[41]	YanC,ChenJ,WangC,et al.Milk exosomes-mediated miR-31-5p delivery accelerates diabetic wound healing through promoting angiogenesis[J].Drug Deliv,2022,29(1):214-228.DOI: 10.1080/10717544.2021.2023699.
[42]	YangC,LuoL,BaiX,et al.Highly-expressed micoRNA-21 in adipose derived stem cell exosomes can enhance the migration and proliferation of the HaCaT cells by increasing the MMP-9 expression through the PI3K/AKT pathway[J].Arch Biochem Biophys,2020,681:108259.DOI: 10.1016/j.abb.2020.108259.
[43]	Rodríguez-RodríguezN,Martínez-JiménezI,García-OjalvoA,et al.Wound chronicity, impaired immunity and infection in diabetic patients[J].MEDICC Rev,2022,24(1):44-58.DOI: 10.37757/MR2021.V23.N3.8.