Abstract: Objective To explore the protective effect of a human proteinkinase inhibitor - Ulinastatin ( UTI) on the prevention of inflammatory mediators oxidative free radical production and changes in intestinal mucosa permeability in mini - pigs at early postburn stages. Methods Twelve Guizhou male mini - pigs were randomly divided into A (control, n =6) and B( with UTI treatment, n = 6) groups. The mini-pigs were inflicted with 35% TBSA full thickness burn on the back and received fluid resuscitation immediately after injury. UTI(5 000 U/kg, 3 times a day) were administered to group B one hour after injury, while an equal amount of normal saline were administered to group A untill the pigs were sacrified. Blood samples were harvested for the determination of serum levels of TNFα , IL-6 , MDA , SOD, DAO, D-lactate at pre- (as normal control) and 6,24,48 ,72 postburn hours( PBHs). Results The serum levels of TNFα and IL-6 in each group at 6 PBH were increased dramatically, especially in group A, peaked at 24 PBH, and seemed to decrease thereafter. The serum levels of MDA and the SOD consumption in group A at 6 - 72 PBH increased obviously than that in normal control. The MDA content in each time points after burn in group B decreased and the SOD consumption increased dramatically when compared with those in group A( P <0.05). The serum DAO activity and the D - lactate content of the rats were significantly increased after burns with barrier function of the intestinal mucosa being severely damaged. The intestinal functions of the rats in group B was markedly improved with functional enzymes obviously decreased. Conclusion UTI can significantly lessen the production of inflammatory.