Abstract: Objective To explore the effects of oral administration of mixed enteral nutritional agent on intestinal mucosal barrier of patients with severe burn injury at early stage. Methods Twenty–four patients with severe burn injury admitted to our burn ward from August 2013 to September 2014, conforming to the study criteria, were divided into conventional therapy group (n=12) and early enteral feeding group (n=12) according to the random number table. Patients in conventional therapy group received conventional treatment immediately after admission, while those in early enteral feeding group were orally given 100 mL of a mixture of glutamine, probiotics, and prebiotics once a day besides conventional treatment for 7 days. Serum levels of diamine oxidase (DAO) and procalcitonin (PCT) and plasma level of LPS were determined by ELISA before treatment and on treatment day (TD) 1, 3, 7, 14, and 21. Wound secretion and blood samples were collected for bacterial culture within the 21 TD. The incidence of MODS within the 21 TD was observed. Data were processed with Fisher′s exact test, rank sum test, analysis of variance for repeated measurement, and LSD–t test. Results (1) Serum levels of DAO in patients of early enteral feeding group on TD 7, 14, and 21 were respectively (14.9±3.7), (12.4±3.1), and (9.5±0.7) ng/mL, which were significantly lower than those of conventional therapy group [(17.5±4.0), (16.3±3.3), and (13.0±1.1) ng/mL, with t values from 2.913 to 15.304, P values below 0.01]. Serum levels of DAO at the other time points were close between the two groups (with t values from –0.598 to 0.139, P values above 0.05). (2) Compared with serum levels of PCT in patients of conventional therapy group [(11.7±20.9) and (12.9±23.9) ng/mL], those of early enteral feeding group were significantly lower on TD 7 and 14 [(2.7±8.1) and (2.0±5.6) ng/mL, with Z values respectively –2.919 and –2.139, P<0.05 or P<0.01]. Serum levels of PCT at the other time points were close between the two groups (with Z values from –1.833 to –0.346, P values above 0.05). (3) Plasma level of LPS in patients of early enteral feeding group on TD 7 was (33±56) pg/mL, which was significantly lower than that of conventional therapy group [(102±108) pg/mL, Z=–2.046, P<0.05]. Plasma levels of LPS at the other time points between the two groups showed no significant difference (with Z values from –2.003~–0.526, P values above 0.05). (4) Positive results in bacterial culture of wound secretion were approximately the same between the two groups (P>0.05). Bacterial culture of blood was positive in 7 patients of conventional therapy group and 1 patient of early enteral feeding group, showing significantly statistical difference (P<0.05). MODS was observed in 1 patient of conventional therapy group, showing no significantly statistical difference with that of early enteral feeding group (no patient, P>0.05). Conclusions Early intestinal feeding of mixed enteral nutritional agent in addition to conventional therapy can effectively promote repair of the impairment of intestinal mucosal barrier, protect integrity of intestinal mucosa, reduce damage to intestines, and alleviate inflammatory response in patients suffering from severe burn injury.