Effects of collagen type ⅩⅦ α1 on epidermal stem cells in aging skin and the microRNA intervention mechanism

Sun Jiachen; Sun Tianjun; Shen Chuan'an; Zhao Hongqing; Liu Xinzhu; Zhang Yijie

doi:10.3760/cma.j.cn501120-20210829-00293

Volume 38 Issue 9

Sep. 2022

Turn off MathJax

Article Contents

Abstract

References

Article Navigation > CHINESE JOURNAL OF BURNS AND WOUNDS > 2022 > 38(9): 839-848

Shi LL,Zhang RF,Xiao H.Roles of interleukin-6/signal transduction and activator of transcription 3 pathway and β-catenin in mechanical stress-induced hypertrophic scar formation in mice[J].Chin J Burns,2021,37(7):647-653.DOI: 10.3760/cma.j.cn501120-20200417-00231.

Citation:

Sun JC,Sun TJ,Shen CA,et al.Effects of collagen type ⅩⅦ α1 on epidermal stem cells in aging skin and the microRNA intervention mechanism[J].Chin J Burns Wounds,2022,38(9):839-848.DOI: 10.3760/cma.j.cn501120-20210829-00293.

Citation:

PDF( 4738 KB)

Effects of collagen type ⅩⅦ α1 on epidermal stem cells in aging skin and the microRNA intervention mechanism

doi: 10.3760/cma.j.cn501120-20210829-00293

Department of Burns and Plastic Surgery, the Fourth Medical Center of PLA General Hospital, Beijing 100048, China

Funds:

Health Special Project of Military Logistics Scientific Research Project 21BJZ29

Major Program of Military Logistics Research Plan ALB18J001

"13th Five-year Plan" Military Key Discipline Professional Construction Project A350109

Scientific Research and Cultivation Program for Health Development in Haidian District of Beijing HP2021-04-80502

More Information

Corresponding author: Shen Chuan'an, Email: shenchuanan@126.com
Received Date: 2021-08-29

Abstract

Abstract

Objective To investigate the expression and function of collagen type ⅩⅦ α1 (COL17α1) in aging mouse skin and its effect on the stemness and proliferation of human epidermal stem cells (ESCs), and to explore the mechanism of related microRNA (miR) in intervening the expression of COL17α1 of human ESC. Methods The method of experimental research was used. Twelve 2-month-old (young) and twelve 24-month-old (aged) male C57BL/6J mice were selected, and full-thickness skin samples from their upper back were taken for follow-up detection. After hematoxylin-eosin staining of the full-thickness skin samples of young mice and aged mice, the structure of the epidermis was observed and the thickness of the epidermis was measured; the morphology of epidermal basement membrane and hemidesmosomes were observed by transmission electron microscopy, and the hemidesmosomes were counted; the mRNA and protein expressions of COL17α1 were detected by real-time fluorescent quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blotting respectively, and the protein expression and distribution of COL17α1 was observed and detected by immunofluorescence method. The fresh foreskin tissue discarded after surgery was obtained from 3 healthy men aged 20-30 years who underwent circumcision at the Fourth Medical Center of PLA General Hospital, ESCs were extracted and well-grown cells were wsed for follow-up experiments. According to the random number table (the same grouping method below), ESCs were divided into blank control group, transfection reagent control group, empty vector plasmid group, and COL17α1 knockdown plasmid group with corresponding treatment. After 48 hours of culture, the mRNA expression of COL17α1 was detected by real-time fluorescent quantitative RT-PCR, the protein expressions of COL17α1 and cytokeratin 14 (CK14) were detected by Western blotting, and the cell proliferation level was detected by cell counting kit 8. miRs that might act on the 3' non-coding region of COL17α1 mRNA were screened through DIANA, miRTarBase, miRNAMap, TargetScan, and microRNA databases. The ESCs were divided into negative control group transfected with miR mimic negative control and each miR mimic group transfected with each of the previously screened miR mimics. Forty-eight hours after transfection, the protein expression of COL17α1 was detected by Western blotting. Based on the sequencing data set GSE114006 in Gene Expression Omnibus (GEO), the GEO2R tool was used to statistically analyze the expression of the previously screened miRs that could cause the reduction of COL17α1 protein expression in the skin of 30 young (18-25 years old) and 30 elderly (>70 years old) human skins. The full-thickness skin samples of young mice and aged mice were taken, and the expressions of increased miRs in the aforementioned aged human skin were detected by real-time fluorescent quantitative RT-PCR. Two batches of human ESCs were taken, the first batch was divided into COL17α1 wild type+miR-203b-3p negative control group and COL17α1 wild type+miR-203b-3p mimic group, and the second batch was divided into COL17α1 mutant+miR-203b-3p negative control group and COL17α1 mutant+miR-203b-3p mimic group. Each group of ESC was transfected with corresponding sequences respectively. Forty-eight hours later, the luciferase reporter gene detection kit was used to detect the gene expression level of COL17α1. The number of samples in the tissue experiment was 6, and the number of samples in the cell experiment was 3. Data were statistically analyzed with independent sample t test, one-way analysis of variance, least significant difference test or Dunnett's test, Mann-Whitney U test or Kruskal-Wallis H test. Results Compared with those of young mice, the boundary between the epidermis and the dermis of the aged mice skin was blurred and the cell layers were less, and the thickness of epidermis was significantly thinner (Z=-2.88, P<0.01); the morphology of basement membrane was discontinuous, with less unevenly distributed hemidesmosomes at the epidermis-dermis junction, and the number of hemidesmosomes was significantly reduced (Z=-2.91, P<0.01); the mRNA and protein expression levels of COL17α1 in the skin of aged mice were significantly decreased (with t values of 10.61 and 6.85, respectively, P<0.01). Compared with those of young mice, the protein expression of COL17α1 in the basal layer of epidermis and the bulb of hair follicle in the skin of aged mice was significantly decreased (Z=-2.24, P<0.05). After 48 hours of culture, the protein expression levels of COL17α1 in ESCs of blank control group, transfection reagent control group, empty vector plasmid group, and COL17α1 knockdown plasmid group were 1.00±0.27, 1.12±0.21, 1.13±0.23, and 0.42±0.18, respectively. Compared with those of blank control group, the mRNA and protein expression levels of COL17α1, the protein expression level of CK14, and the proliferation level of ESCs in transfection reagent control group and empty vector plasmid group did not change significantly (P>0.05), while these indexes in COL17α1 knockdown plasmid group were significantly decreased (P<0.05 or P<0.01). miR-203a-3p, miR-203b-3p, miR-512-5p, miR-124-3p, miR-28-5p, miR-590-3p, and miR-329-5p might bind to the 3' non-coding region of COL17α1 mRNA. Forty-eight hours after transfection, compared with 1.000±0.224 in negative control group, the protein expression level of COL17α1 in ESCs of miR-329-5p mimic group, miR-203b-3p mimic group, and miR-203a-3p mimic group decreased significantly (0.516±0.188, 0.170±0.025, and 0.235±0.025, with t values of 3.17, 5.43, and 5.07, respectively, P<0.05 or P<0.01). Only the expression level of miR-203b-3p in the skin of the elderly was significantly higher than that of the young (t=3.27, P<0.01). The expression level of miR-203b-3p in the skin of aged mice was significantly higher than that of young mice (Z=-2.88, P<0.01). Forty-eight hours after transfection, the gene expression level of COL17α1 in ESCs of COL17α1 wild type+miR-203b-3p mimic group was significantly lower than that of COL17α1 wild type+miR-203b-3p negative control group (t=7.66, P<0.01). The gene expression level of COL17α1 in ESCs of COL17α1 mutant+miR-203b-3p mimic group was similar to that of COL17α1 mutant+miR-203b-3p negative control group (P>0.05). Conclusions The mRNA and protein expression levels of COL17α1 decrease with age increasing in mice, which may lead to the detachment of mouse ESC from the epidermal basement membrane. Decreased expression of COL17α1 can inhibit the expression of CK14 and ESC proliferation, which may be responsible for the thinning of the epidermis and slower wound healing in aged human skin. The increased expression of miR-203b-3p in aged mouse skin can target and bind to the 3' non-coding region of COL17α1 mRNA, hindering the post-transcriptional translation process, thus resulting in decreased COL17α1 protein expression.
- Aging,
- Skin,
- Hemidesmosomes,
- Collagen type XVII α1,
- Epidermal stem cells,
- microRNA

FullText(HTML)

References(30)

References

[1]	WeiX,LiM,ZhengZ,et al.Senescence in chronic wounds and potential targeted therapies[J/OL].Burns Trauma,2022,10:tkab045[2022-08-10].https://pubmed.ncbi.nlm.nih.gov/35187179/.DOI: 10.1093/burnst/tkab045.
[2]	GoeiH,van BaarME,DokterJ,et al.Burns in the elderly: a nationwide study on management and clinical outcomes[J/OL].Burns Trauma,2020,8:tkaa027[2022-08-10].https://pubmed.ncbi.nlm.nih.gov/33123606/.DOI: 10.1093/burnst/tkaa027.
[3]	MahmoudiS,ManciniE,XuL,et al.Heterogeneity in old fibroblasts is linked to variability in reprogramming and wound healing[J].Nature,2019,574(7779):553-558.DOI: 10.1038/s41586-019-1658-5.
[4]	FuX.Wound healing center establishment and new technology application in improving the wound healing quality in China[J/OL].Burns Trauma,2020,8:tkaa038[2021-08-29]. https://pubmed.ncbi.nlm.nih.gov/33134399/. DOI: 10.1093/burnst/tkaa038.
[5]	XieJ,YaoB,HanY,et al.Skin appendage-derived stem cells: cell biology and potential for wound repair[J/OL].Burns Trauma,2016,4:38[2022-08-10].https://pubmed.ncbi.nlm.nih.gov/27800498/.DOI: 10.1186/s41038-016-0064-6.
[6]	NegriVA,WattFM.Understanding human epidermal stem cells at single-cell resolution[J].J Invest Dermatol,2022,142(8):2061-2067.DOI: 10.1016/j.jid.2022.04.003.
[7]	HsuYC,FuchsE.Building and maintaining the skin[J].Cold Spring Harb Perspect Biol,2022,14(7):a040840.DOI: 10.1101/cshperspect.a040840.
[8]	HarnHI,ChenCC,WangSP,et al.Tissue mechanics in haired murine skin: potential implications for skin aging[J].Front Cell Dev Biol,2021,9:635340.DOI: 10.3389/fcell.2021.635340.
[9]	NatsugaK,WatanabeM,NishieW,et al.Life before and beyond blistering: the role of collagen XVII in epidermal physiology[J].Exp Dermatol,2019,28(10):1135-1141.DOI: 10.1111/exd.13550.
[10]	TanimuraS,TadokoroY,InomataK,et al.Hair follicle stem cells provide a functional niche for melanocyte stem cells[J].Cell Stem Cell,2011,8(2):177-187.DOI: 10.1016/j.stem.2010.11.029.
[11]	Umegaki-AraoN,PasmooijAM,ItohM,et al.Induced pluripotent stem cells from human revertant keratinocytes for the treatment of epidermolysis bullosa[J].Sci Transl Med,2014,6(264):264ra164.DOI: 10.1126/scitranslmed.3009342.
[12]	HérisséAL,CharlesworthA,BellonN,et al.Genotypic and phenotypic analysis of 34 cases of inherited junctional epidermolysis bullosa caused by COL17A1 mutations[J].Br J Dermatol,2021,184(5):960-962.DOI: 10.1111/bjd.19752.
[13]	MatsumuraH,MohriY,BinhNT,et al.Hair follicle aging is driven by transepidermal elimination of stem cells via COL17A1 proteolysis[J].Science,2016,351(6273):aad4395.DOI: 10.1126/science.aad4395.
[14]	WangX,ShenC,LiZ,et al.Efficient isolation and high yield of epidermal cells from foreskin biopsies by dynamic trypsinization[J].Burns,2018,44(5):1240-1250.DOI: 10.1016/j.burns.2018.01.013.
[15]	LiB,TangH,BianX,et al.Calcium silicate accelerates cutaneous wound healing with enhanced re-epithelialization through EGF/EGFR/ERK-mediated promotion of epidermal stem cell functions[J/OL].Burns Trauma,2021,9:tkab029[2022-08-10].https://pubmed.ncbi.nlm.nih.gov/34604395/.DOI: 10.1093/burnst/tkab029.
[16]	LeeH,HongY,KimM.Structural and functional changes and possible molecular mechanisms in aged skin[J].Int J Mol Sci,2021,22(22):12489.DOI: 10.3390/ijms222212489.
[17]	SedovE,KorenE,ChopraS,et al.THY1-mediated mechanisms converge to drive YAP activation in skin homeostasis and repair[J].Nat Cell Biol,2022,24(7):1049-1063.DOI: 10.1038/s41556-022-00944-6.
[18]	KulukianA,FuchsE.Spindle orientation and epidermal morphogenesis[J].Philos Trans R Soc Lond B Biol Sci,2013,368(1629):20130016.DOI: 10.1098/rstb.2013.0016.
[19]	ChoiK,ParkSH,ParkSY,et al.The stem cell quiescence and niche signaling is disturbed in the hair follicle of the hairpoor mouse, an MUHH model mouse[J].Stem Cell Res Ther,2022,13(1):211.DOI: 10.1186/s13287-022-02898-w.
[20]	PersaOD,KoesterJ,NiessenCM.Regulation of cell polarity and tissue architecture in epidermal aging and cancer[J].J Invest Dermatol,2021,141(4S):S1017-1023.DOI: 10.1016/j.jid.2020.12.012.
[21]	LangtonAK,HalaiP,GriffithsCE,et al.The impact of intrinsic ageing on the protein composition of the dermal-epidermal junction[J].Mech Ageing Dev,2016,156:14-16.DOI: 10.1016/j.mad.2016.03.006.
[22]	朱冬振,姚斌,崔晓丽,等.年龄对人增生性瘢痕硬度和成纤维细胞纤维化表型的影响及其机制[J].中华烧伤杂志,2021,37(10):937-945.DOI: 10.3760/cma.j.cn501120-20200810-00374.
[23]	WangY,KitahataH,KosumiH,et al.Collagen XVII deficiency alters epidermal patterning[J].Lab Invest,2022,102(6):581-588.DOI: 10.1038/s41374-022-00738-2.
[24]	KwonOS,YooHG,HanJH,et al.Photoaging-associated changes in epidermal proliferative cell fractions in vivo[J].Arch Dermatol Res,2008,300(1):47-52.DOI: 10.1007/s00403-007-0812-3.
[25]	XiangY,LiuY,YangY,et al.Reduced expression of collagen 17A1 in naturally aged, photoaged, and UV-irradiated human skin in vivo: potential links to epidermal aging[J/OL].J Cell Commun Signal,2022,16(3):421-432.DOI: 10.1007/s12079-021-00654-y.
[26]	廖银友,张丕红.竞争性内源性RNA在创面愈合中作用的研究进展[J].中华烧伤与创面修复杂志,2022,38(1):84-89.DOI: 10.3760/cma.j.cn501120-20201125-00498.
[27]	AakkoS,StraumeAH,BirkelandEE,et al.MYC-induced miR-203b-3p and miR-203a-3p control Bcl-xL expression and paclitaxel sensitivity in tumor cells[J].Transl Oncol,2019,12(1):170-179.DOI: 10.1016/j.tranon.2018.10.001.
[28]	LabarradeF,BottoJM,ImbertIM.miR-203 represses keratinocyte stemness by targeting survivin[J/OL].J Cosmet Dermatol,2022(2022-06-08)[2022-08-10].https://pubmed.ncbi.nlm.nih.gov/35673958/.DOI:10.1111/jocd.15147.[published online ahead of print].
[29]	孙礼祥,吴帅,张小薇,等.基于单细胞RNA测序探讨小鼠全层皮肤缺损创面中真皮成纤维细胞的生长因子调控网络[J].中华烧伤与创面修复杂志,2022,38(7):629-639.DOI: 10.3760/cma.j.cn501225-20220215-00029.
[30]	WangZ,ShiC.Cellular senescence is a promising target for chronic wounds: a comprehensive review[J/OL].Burns Trauma,2020,8:tkaa021[2022-08-10].https://pubmed.ncbi.nlm.nih.gov/32607375/.DOI: 10.1093/burnst/tkaa021.

Relative Articles

[1]	Xing Peipeng, Xue Jidong, Guo Haina, Ma Chao, Zhao Xiaokai, Liang Zhanling, Dong Guoyun, Di Haiping, Xia Chengde. Clinical application effect of bypass vein bridging in repairing high-voltage electric burn wounds on the head with free anterolateral thigh flaps[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2024, 40(8): 725-731. doi: 10.3760/cma.j.cn501225-20240419-00142
[2]	Yin Shanqing, Zhu Feng, Huang Yaopeng, Pan Jiadong, Xiao Dongchao, Liu Linhai, Li Xueyuan, Wang Xin. Effects of thinned anterolateral thigh perforator flaps combined with finger splitting and webplasty in sequential treatment of degloving destructive wound of total hand[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2024, 40(11): 1052-1058. doi: 10.3760/cma.j.cn501225-20240723-00275
[3]	Wang Shaohua, Wang Shunbin, Xu Zhaorong, Chen Zhaohong. Clinical effects of antegrade anterolateral thigh pedicled flaps in repairing wounds in the perineum or inguinal regions[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2024, 40(10): 978-984. doi: 10.3760/cma.j.cn501225-20240218-00064
[4]	Liu Fei, Yan Weiqi, Ma Qiang, Liu Yibin, Yang Zhibin. Clinical effect of anterolateral thigh flow-through chimeric perforator free flap transplantation in the treatment of upper limb complex tissue defects with main artery injury[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2024, 40(2): 172-179. doi: 10.3760/cma.j.cn501225-20231103-00176
[5]	Wang Shi, Dong Shuai, Cao Yang, Wang Guiyang, Yang Chengpeng, Sun Fengwen, Huang Yongtao, Guo Liping, Yang Liang, Zhou Rong, Ju Jihui. Application of highly selective arterial indocyanine green angiography in the design of anterolateral thigh free flap[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2024, 40(10): 948-954. doi: 10.3760/cma.j.cn501225-20240513-00174
[6]	Fang Jun, Zhao Guangzong, Li Huazhuang, Zhang Longqiang, Liang Zhiyong, Li Xueqin. Effects of three-dimensional computed tomography angiography-assisted free medial sural artery perforator flap in repairing foot wounds[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2023, 39(4): 343-349. doi: 10.3760/cma.j.cn501225-20220930-00430
[7]	Wang Chengde, Wang Ai, Sun Jiling, Ma Wenguo, Wang Jianguo. Clinical effects of free peroneal artery perforator flaps in repairing forefoot skin and soft tissue defect wounds assisted with three-dimensional computed tomography angiography[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2022, 38(7): 661-666. doi: 10.3760/cma.j.cn501120-20210914-00317
[8]	Zhao Shuming, Liu Yaming, Liu Na, Zhang Hongliang, Song Zhanfeng, Gao Wenhua, Lan Yuehui, Fan Anwei, Liu Xueliang. Clinical effects of retrograde anterolateral thigh perforator flaps assisted with computed tomography angiography in repairing skin and soft tissue defects around the knee or in proximal lower leg[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2021, 37(4): 356-362. doi: 10.3760/cma.j.cn501120-20200905-00401
[9]	Han Fu, Zheng Zhao, Wang Hongtao, Guan Hao, Ji Peng, Hu Xiaolong, Tong Lin, Zhang Zhi, Chen Qiaohua, Feng Aina, Hu Dahai. Effects of anterolateral thigh free flap with fascia lata in repairing dura mater defect after resection of head squamous cell carcinoma[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2020, 36(3): 219-223. doi: 10.3760/cma.j.cn501120-20190505-00222
[10]	Yang Li, Cai Bin, Xue Junrong, Jiang Peng, Guo Xianzhao. Clinical effects of individualized free anterolateral thigh flap in repairing complex refractory wound[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2020, 36(8): 730-734. doi: 10.3760/cma.j.cn501120-20190621-00281
[11]	Xing Peipeng, Guo Haina, Di Haiping, Xue Jidong, Cao Dayong, Liang Zhanling, Liang Yan, Xia Chengde. Clinical effect of free anterolateral thigh flap combined with arterial vascular reconstruction on repairing high-voltage electrical burn wound on the wrist[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2020, 36(6): 419-425. doi: 10.3760/cma.j.cn501120-20200219-00067
[12]	Ma Wenguo, Wang Chengde, Wang Ai, Liu Fei. Effect of free medial plantar perforator flap in repairing deep burn wound on palm with the assistance of three dimensional computed tomography angiography[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2020, 36(4): 323-326. doi: 10.3760/cma.j.cn501120-20190308-00091
[13]	Xia Chengde, Di Haiping, Xing Peipeng, Xue Jidong, Cao Dayong, Tian Shemin, Wang Limin, Feng Ke, Zhao Yaohua. Clinical effect of free anterolateral thigh flap in repairing large annular soft tissue defect of lower leg after burn[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2019, 35(4): 248-252. doi: 10.3760/cma.j.issn.1009-2587.2019.04.003
[14]	Xiong Sheng, Ju Jihui, Jin Guangzhe, Zhu Congkun, Zhang Guangliang, Tang Linfeng, Zhou Guangliang. Multiple free homologous superficial peroneal artery perforator flaps of crus for repair of multiple hand wounds[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2019, 35(9): 655-660. doi: 10.3760/cma.j.issn.1009-2587.2019.09.003
[15]	Lin Jian, Zhang Tianhao, Hu Deqing, Wang Zhijiang, Liu Caiyue, Zheng Heping. Clinical effects of dorsal perforator fascia pedicle flap of the deep palmar arch in the repair of skin and soft tissue defects of finger web area[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2019, 35(7): 490-494. doi: 10.3760/cma.j.issn.1009-2587.2019.07.003
[16]	Xia Chengde, Xue Jidong, Di Haiping, Han Dawei, Cao Dayong, Li Qiang, Jing Fuqin, Niu Xihua. Application effects of CT angiography and three-dimensional reconstruction technique in repairing scar around the mouth and chin with expanded forehead axial flap[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2018, 34(10): 677-682. doi: 10.3760/cma.j.issn.1009-2587.2018.10.006
[17]	Yang Li, Fang Bairong, He Jiyong, Wang Xiancheng. Establishment and application of three-dimensional model of deep inferior epigastric artery perforator flap based on computed tomography angiography[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2018, 34(5): 297-302. doi: 10.3760/cma.j.issn.1009-2587.2018.05.010
[18]	Yang Zhibin, Niu Jiandong, Ma Yong, Li Jinning, Shen Jiangyong, Yao Ming. Clinical application of computed tomography angiography and three-dimensional reconstruction in repairing high-voltage electrical burn wounds in necks, shoulders, axillas, and upper arms with tissue flaps[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2018, 34(12): 874-880. doi: 10.3760/cma.j.issn.1009-2587.2018.12.011

Supplements(0)

Cited By

Proportional views

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Figures(7) / Tables(1)

Get Citation

PDF

XML

Article Metrics

Article views (308) PDF downloads(68)

Effects of collagen type ⅩⅦ α1 on epidermal stem cells in aging skin and the microRNA intervention mechanism

doi: 10.3760/cma.j.cn501120-20210829-00293

Abstract

References

Relative Articles

Proportional views

Catalog

通讯作者: 陈斌, bchen63@163.com

Article Metrics

Proportional views

Related

Effects of collagen type ⅩⅦ α1 on epidermal stem cells in aging skin and the microRNA intervention mechanism

doi: 10.3760/cma.j.cn501120-20210829-00293

Abstract

References

Relative Articles

Proportional views

Catalog

通讯作者: 陈斌, bchen63@163.com

Article Metrics

Proportional views

Related

Export File

Citation

Format

Content