Qi Xinxin, Yang Yunxi, Sun Bingwei. Early changes of chemotactic function of peripheral blood neutrophil of patients with severe burns and the influence factor[J]. Chin j Burns, 2020, 36(3): 204-209. Doi: 10.3760/cma.j.cn501120-20190801-00329
Citation: Yang YX,Huang JM,Liu L,et al.Regulatory effects of the Nocardia rubra cell wall skeleton on the biological function of human neutrophils[J].Chin J Burns Wounds,2023,39(7):625-632.DOI: 10.3760/cma.j.cn501225-20230223-00056.

Regulatory effects of the Nocardia rubra cell wall skeleton on the biological function of human neutrophils

doi: 10.3760/cma.j.cn501225-20230223-00056
Funds:

Key Program of Joint Fund of National Natural Science Foundation of China U21A20370

General Program of National Natural Science Foundation of China 82072217, 81772135

Natural Science Foundation of Jiangsu Province of China BK20201178

More Information
  •   Objective   To investigate the regulatory effects and mechanism of Nocardia rubra cell wall skeleton (Nr-CWS) on the biological function of human neutrophils.   Methods   The experimental research method was used. Fifteen healthy adult volunteers (7 males and 8 females, aged 24 to 45 years) were recruited from Suzhou Physical Examination Center for physical examination from May to October 2022, the peripheral venous blood was collected, and neutrophils were extracted by immunomagnetic bead sorting. The cells were divided into normal control group without any treatment, Nr-CWS alone group treated with Nr-CWS of final mass concentration 60 ng/mL alone, endotoxin/lipopolysaccharide (LPS) alone group stimulated with LPS of final mass concentration 1 μg/mL alone, and LPS+Nr-CWS group stimulated with LPS first and then treated with Nr-CWS as before. After 1 h of culture, the chemotaxis distance, chemotactic cell percentage, chemotactic index, maximum chemotactic speed, and chemotactic function score of neutrophils were detected using the modified agarose chemotactic model; the proportion and fluorescence intensity of phagocytosis cells, the level of reactive oxygen species (ROS), the protein expression levels of granular protein CD35, CD66b, and CD63, and the concentrations of inflammatory cytokines of interleukin 2 (IL-2), IL-4, IL-6, IL-10, IL-17A, tumor necrosis factor alpha (TNF-α), and interferon-γ in cell culture supernatant were detected by flow cytometry. The number of samples in each group in the above experiments was 15. Data were statistically analyzed with analysis of variance for factorial design and independent sample t test.   Results   After 1 h of culture, the chemotactic function score of cells in normal control group, Nr-CWS alone group, LPS alone group, and LPS+Nr-CWS group were 15.0, 14.5±0.5, 1.5±0.5, 12.0±1.5, respectively. Compared with those in normal control group, the chemotaxis distance, chemotactic cell percentage, chemotactic index, maximum chemotactic speed, and chemotactic function score of cells were significantly decreased in LPS alone group and LPS+Nr-CWS group (with t values of 18.36, 18.88, 54.28, 18.36, 46.77, 10.58, 14.74, 6.84, 10.58, and 4.24, respectively, P<0.05); compared with those in LPS alone group, the five chemotactic function indexes as above in LPS+Nr-CWS group were significantly increased (with t values of 11.47, 14.65, 11.62, 11.47, and 13.75, respectively, P<0.05). After 1 h of culture, compared with those in normal control group, the proportion and fluorescence intensity of phagocytosis cells were significantly increased in Nr-CWS alone group (with t values of 6.86 and 6.73, respectively, P<0.05), and the above two indexes were significantly decreased in LPS alone group (with t values of 7.35 and 22.72, respectively, P<0.05) and LPS+Nr-CWS group (with t values of 21.37 and 13.10, respectively, P<0.05). After 1 h of culture, compared with that in normal control group, the level of ROS of cells in LPS alone group was significantly increased ( t=6.64, P<0.05); compared with that in LPS alone group, the level of ROS of cells in LPS+Nr-CWS group was significantly decreased ( t=5.46, P<0.05). After 1 h of culture, compared with those in normal control group, the protein expressions of CD35, CD66b, and CD63 of cells were significantly increased in LPS alone group and LPS+Nr-CWS group (with t values of 16.75, 17.45, 10.82, 5.70, 19.35, and 15.37, respectively, P<0.05); compared with those in LPS alone group, the protein expressions of CD35, CD66b, and CD63 of cells were significantly decreased in LPS+Nr-CWS group (with t values of 4.92, 5.72, and 3.18, respectively, P<0.05). After 1 h of culture, compared with those in normal control group, the concentrations of IL-2, IL-4, IL-6, IL-10, IL-17A, TNF-α, and interferon-γ in cell culture supernatant were significantly increased in LPS alone group (with t values of 22.10, 9.50, 7.21, 10.22, 24.88, 8.43, and 47.48, respectively, P<0.05), and the concentrations of IL-6, IL-10, IL-17A, TNF-α, and interferon-γ in cell culture supernatant were significantly increased in LPS+Nr-CWS group (with t values of 4.68, 5.12, 8.02, 5.58, and 7.13, respectively, P<0.05); compared with those in LPS alone group, the concentrations of IL-2, IL-4, IL-6, IL-10, IL-17A, TNF-α, and interferon-γ in cell culture supernatant were significantly decreased in LPS+Nr-CWS group (with t values of 5.39, 2.83, 5.79, 2.90, 5.87, 4.88, and 39.64, respectively, P<0.05).   Conclusions   Nr-CWS can enhance the phagocytosis ability of neutrophils in normal condition and improve the chemotactic function, ROS level, degranulation protein level, and inflammatory factor level of human neutrophils in infectious condition. Nr-CWS can enhance the anti-infection ability of human neutrophils by regulating its biological behavior in innate immunity.

     

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    Created with Highcharts 5.0.7Chart context menuAccess Area Distribution其他: 2.4 %其他: 2.4 %其他: 0.1 %其他: 0.1 %三明: 0.3 %三明: 0.3 %三门峡: 0.3 %三门峡: 0.3 %上海: 0.4 %上海: 0.4 %丽水: 2.2 %丽水: 2.2 %伦敦: 0.3 %伦敦: 0.3 %佛山: 0.1 %佛山: 0.1 %六安: 0.1 %六安: 0.1 %兰州: 0.1 %兰州: 0.1 %内江: 0.1 %内江: 0.1 %包头: 0.6 %包头: 0.6 %北京: 1.7 %北京: 1.7 %南京: 0.8 %南京: 0.8 %南宁: 0.1 %南宁: 0.1 %南平: 0.2 %南平: 0.2 %厦门: 0.7 %厦门: 0.7 %台州: 1.2 %台州: 1.2 %合肥: 0.1 %合肥: 0.1 %吉林: 1.0 %吉林: 1.0 %呼和浩特: 0.7 %呼和浩特: 0.7 %咸阳: 1.4 %咸阳: 1.4 %哥伦布: 0.1 %哥伦布: 0.1 %嘉兴: 1.0 %嘉兴: 1.0 %大连: 1.9 %大连: 1.9 %天津: 0.2 %天津: 0.2 %宁德: 0.3 %宁德: 0.3 %宁波: 1.2 %宁波: 1.2 %安康: 0.4 %安康: 0.4 %宜春: 0.2 %宜春: 0.2 %宿迁: 1.4 %宿迁: 1.4 %常德: 1.3 %常德: 1.3 %广安: 0.2 %广安: 0.2 %广州: 2.6 %广州: 2.6 %张家口: 1.4 %张家口: 1.4 %徐州: 0.7 %徐州: 0.7 %德州: 0.1 %德州: 0.1 %成都: 0.2 %成都: 0.2 %扬州: 0.3 %扬州: 0.3 %抚州: 0.9 %抚州: 0.9 %新乡: 0.1 %新乡: 0.1 %无锡: 0.6 %无锡: 0.6 %日照: 0.7 %日照: 0.7 %昆明: 0.4 %昆明: 0.4 %普赖恩维尔: 0.7 %普赖恩维尔: 0.7 %朝阳: 1.1 %朝阳: 1.1 %本溪: 1.4 %本溪: 1.4 %杭州: 0.6 %杭州: 0.6 %格兰特县: 0.1 %格兰特县: 0.1 %梧州: 0.2 %梧州: 0.2 %武汉: 1.0 %武汉: 1.0 %汕头: 1.0 %汕头: 1.0 %池州: 0.4 %池州: 0.4 %沃思堡: 0.3 %沃思堡: 0.3 %沈阳: 0.8 %沈阳: 0.8 %泉州: 0.1 %泉州: 0.1 %泰州: 0.3 %泰州: 0.3 %泸州: 0.1 %泸州: 0.1 %济南: 0.8 %济南: 0.8 %海口: 0.1 %海口: 0.1 %海得拉巴: 0.3 %海得拉巴: 0.3 %海西: 0.2 %海西: 0.2 %淮北: 0.2 %淮北: 0.2 %淮南: 0.2 %淮南: 0.2 %淮安: 0.7 %淮安: 0.7 %深圳: 0.1 %深圳: 0.1 %温州: 0.7 %温州: 0.7 %渭南: 0.3 %渭南: 0.3 %湖州: 1.4 %湖州: 1.4 %湘潭: 0.1 %湘潭: 0.1 %湘西: 0.8 %湘西: 0.8 %漳州: 1.3 %漳州: 1.3 %班加罗尔: 0.7 %班加罗尔: 0.7 %盐城: 0.3 %盐城: 0.3 %盘锦: 0.6 %盘锦: 0.6 %石家庄: 0.7 %石家庄: 0.7 %福州: 0.6 %福州: 0.6 %秦皇岛: 0.7 %秦皇岛: 0.7 %绍兴: 1.5 %绍兴: 1.5 %绵阳: 0.1 %绵阳: 0.1 %舟山: 0.4 %舟山: 0.4 %芒廷维尤: 23.4 %芒廷维尤: 23.4 %苏州: 0.7 %苏州: 0.7 %荆州: 0.2 %荆州: 0.2 %荆门: 0.8 %荆门: 0.8 %莆田: 0.1 %莆田: 0.1 %营口: 0.4 %营口: 0.4 %葫芦岛: 0.8 %葫芦岛: 0.8 %葵涌: 0.1 %葵涌: 0.1 %襄阳: 0.6 %襄阳: 0.6 %西宁: 2.9 %西宁: 2.9 %西安: 0.8 %西安: 0.8 %辽阳: 0.4 %辽阳: 0.4 %连云港: 0.2 %连云港: 0.2 %邯郸: 0.2 %邯郸: 0.2 %邵阳: 0.9 %邵阳: 0.9 %郴州: 0.2 %郴州: 0.2 %重庆: 5.4 %重庆: 5.4 %金华: 0.8 %金华: 0.8 %铁岭: 1.5 %铁岭: 1.5 %锦州: 0.1 %锦州: 0.1 %长沙: 0.6 %长沙: 0.6 %随州: 0.2 %随州: 0.2 %雅安: 0.1 %雅安: 0.1 %青岛: 0.1 %青岛: 0.1 %鞍山: 4.6 %鞍山: 4.6 %鹰潭: 0.2 %鹰潭: 0.2 %黄冈: 0.4 %黄冈: 0.4 %黄南: 0.3 %黄南: 0.3 %黄山: 0.2 %黄山: 0.2 %黄石: 0.3 %黄石: 0.3 %其他其他三明三门峡上海丽水伦敦佛山六安兰州内江包头北京南京南宁南平厦门台州合肥吉林呼和浩特咸阳哥伦布嘉兴大连天津宁德宁波安康宜春宿迁常德广安广州张家口徐州德州成都扬州抚州新乡无锡日照昆明普赖恩维尔朝阳本溪杭州格兰特县梧州武汉汕头池州沃思堡沈阳泉州泰州泸州济南海口海得拉巴海西淮北淮南淮安深圳温州渭南湖州湘潭湘西漳州班加罗尔盐城盘锦石家庄福州秦皇岛绍兴绵阳舟山芒廷维尤苏州荆州荆门莆田营口葫芦岛葵涌襄阳西宁西安辽阳连云港邯郸邵阳郴州重庆金华铁岭锦州长沙随州雅安青岛鞍山鹰潭黄冈黄南黄山黄石

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