Email Alerts
RSS
Volume 40 Issue 1
Jan.  2024
Turn off MathJax
Article Contents
Article Navigation >  CHINESE JOURNAL OF BURNS AND WOUNDS  > 2024  >  40(1): 50-56
Liu Y,Cheng F,Wang ZW,et al.Preparation of chitin/hyaluronic acid/collagen hydrogel loaded with mouse adipose-derived stem cells and its effects on wound healing of full-thickness skin defects in rats[J].Chin J Burns Wounds,2024,40(1):50-56.DOI: 10.3760/cma.j.cn501225-20230928-00101.
Citation: Liu Y,Cheng F,Wang ZW,et al.Preparation of chitin/hyaluronic acid/collagen hydrogel loaded with mouse adipose-derived stem cells and its effects on wound healing of full-thickness skin defects in rats[J].Chin J Burns Wounds,2024,40(1):50-56.DOI: 10.3760/cma.j.cn501225-20230928-00101.
shu

Preparation of chitin/hyaluronic acid/collagen hydrogel loaded with mouse adipose-derived stem cells and its effects on wound healing of full-thickness skin defects in rats

doi: 10.3760/cma.j.cn501225-20230928-00101
  • 1.

    Department of Emergency Medicine, Northern Theater Command General Hospital, Shenyang 110016, China

  • 2.

    Laboratory of Acute and Critical Care Research and Transformation, Jilin Provincial People's Hospital, Changchun 130021, China

  • 3.

    Department of Emergency Medicine, Jilin Provincial People's Hospital, Changchun 130021, China

Funds:

Doctoral Research Foundation of Liaoning Province of China 2023-BS-032

More Information
    Abstract
  •   Objective   To prepare the chitin/hyaluronic acid/collagen hydrogel loaded with mouse adipose-derived stem cells and to explore its effects on wound healing of full-thickness skin defects in rats.   Methods   The research was an experimental research. Chitin nanofibers were prepared by acid hydrolysis and alkaline extraction method, and then mixed with hyaluronic acid and collagen to prepare chitin/hyaluronic acid/collagen hydrogels (hereinafter referred to as hydrogels). Besides, the hydrogels loaded with mouse adipose-derived stem cells were prepared. Thirty male 12-week-old guinea pigs were divided into negative control group, positive control group, and hydrogel group according to the random number table, with 10 guinea pigs in each group. Ethanol, 4-aminobenzoic acid ethyl ester, or the aforementioned prepared hydrogels without cells were topically applied on both sides of back of guinea pigs respectively for induced contact and stimulated contact, and skin edema and erythema formation were observed at 24 and 48 h after stimulated contact. Adipose-derived stem cells from mice were divided into normal control group cultured routinely and hydrogel group cultured with the aforementioned prepared hydrogels without cells. After 3 d of culture, protein expressions of platelet-derived growth factor-D (PDGF-D), insulin-like growth factor-Ⅰ (IGF-Ⅰ), and transforming growth factor β 1 (TGF-β 1) were detected by Western blotting ( n=3). Eight male 8-week-old Sprague-Dawley rats were taken and a circular full-thickness skin defect wound was created on each side of the back. The wounds were divided into blank control group without any treatment and hydrogel group with the aforementioned prepared hydrogels loaded with adipose-derived stem cells applied. Wound healing was observed at 0 (immediately), 2, 4, 8, and 10 d after injury, and the wound healing rate was calculated at 2, 4, 8, and 10 d after injury. Wound tissue samples at 10 d after injury were collected, the new tissue formation was observed by hematoxylin-eosin staining; the concentrations of interleukin-1α (IL-1α), IL-6, IL-4, and IL-10 were detected by enzyme-linked immunosorbent assay method; the expressions of CD16 and CD206 positive cells were observed by immunohistochemical staining and the percentages of positive cells were calculated. The sample numbers in animal experiment were all 8.   Results   At 24 h after stimulated contact, no skin edema was observed in the three groups of guinea pigs, and only mild skin erythema was observed in 7 guinea pigs in positive control group. At 48 h after stimulated contact, skin erythema was observed in 8 guinea pigs and skin edema was observed in 4 guinea pigs in positive control group, while no obvious skin erythema or edema was observed in guinea pigs in the other two groups. After 3 d of culture, the protein expression levels of PDGF-D, IGF-I, and TGF-β 1 in adipose-derived stem cells in hydrogel group were significantly higher than those in normal control group (with t values of 12.91, 11.83, and 7.92, respectively, P<0.05). From 0 to 10 d after injury, the wound areas in both groups gradually decreased, and the wounds in hydrogel group were almost completely healed at 10 d after injury. At 4, 8, and 10 d after injury, the wound healing rates in hydrogel group were (38±4)%, (54±5)%, and (69±6)%, respectively, which were significantly higher than (21±6)%, (29±7)%, and (31±7)% in blank control group (with t values of 3.82, 3.97, and 4.05, respectively, Pvalues all <0.05). At 10 d after injury, compared with those in blank control group, the epidermis in wound in hydrogel group was more intact, and there were increases in hair follicles, blood vessels, and other skin appendages. At 10 d after injury, the concentrations of IL-1α and IL-6 in wound tissue in hydrogel group were significantly lower than those in blank control group (with tvalues of 8.21 and 7.99, respectively, P<0.05), while the concentrations of IL-4 and IL-10 were significantly higher than those in blank control group (with tvalues of 6.57 and 9.03, respectively, P<0.05). The percentage of CD16 positive cells in wound tissue in hydrogel group was significantly lower than that in blank control group ( t=8.02, P<0.05), while the percentage of CD206 positive cells was significantly higher than that in blank control group ( t=7.21, P<0.05).   Conclusions   The hydrogel loaded with mouse adipose-derived stem cells is non-allergenic, can promote the secretion of growth factors in adipose-derived stem cells, promote the polarization of macrophages to M2 phenotype in wound tissue in rats with full-thickness skin defects, and alleviate inflammatory reaction, thereby promoting wound healing.

     

    • FullText(HTML)
  • loading
    • References(22)
  • [1]
    QiL,ZhangC,WangB,et al.Progress in hydrogels for skin wound repair[J].Macromol Biosci,2022,22(7):e2100475.DOI: 10.1002/mabi.202100475.
    [2]
    郑力铭,刘钟元,颜鸿宇,等.小檗碱对糖尿病小鼠全层皮肤缺损创面愈合的影响及其机制[J].中华烧伤与创面修复杂志,2023,39(11):1072-1082.DOI: 10.3760/cma.j.cn501225-20230411-00120.
    [3]
    van DongenJA,HarmsenMC,van der LeiB,et al.Augmentation of dermal wound healing by adipose tissue-derived stromal cells (ASC)[J].Bioengineering (Basel),2018,5(4):91. DOI: 10.3390/bioengineering5040091.
    [4]
    AzamM,GhufranH,ButtH,et al.Curcumin preconditioning enhances the efficacy of adipose-derived mesenchymal stem cells to accelerate healing of burn wounds[J/OL].Burns Trauma,2021,9:tkab021[2023-09-28].https://pubmed.ncbi.nlm.nih.gov/34514007/.DOI: 10.1093/burnst/tkab021.
    [5]
    HouL,ZhangX,DuH.Advances in mesenchymal stromal cells and nanomaterials for diabetic wound healing[J].Diabetes Metab Res Rev,2023,39(4):e3638.DOI: 10.1002/dmrr.3638.
    [6]
    MaziniL,RochetteL,AdmouB,et al.Hopes and limits of adipose-derived stem cells (ADSCs) and mesenchymal stem cells (MSCs) in wound healing[J].Int J Mol Sci,2020,21(4):1306.DOI: 10.3390/ijms21041306.
    [7]
    XiaS,WengT,JinR,et al.Curcumin-incorporated 3D bioprinting gelatin methacryloyl hydrogel reduces reactive oxygen species-induced adipose-derived stem cell apoptosis and improves implanting survival in diabetic wounds[J/OL].Burns Trauma,2022,10:tkac001[2023-09-28].https://pubmed.ncbi.nlm.nih.gov/35291229/.DOI: 10.1093/burnst/tkac001.
    [8]
    BaharloueiP,RahmanA.Chitin and chitosan: prospective biomedical applications in drug delivery, cancer treatment, and wound healing[J].Mar Drugs,2022,20(7):460.DOI: 10.3390/md20070460.
    [9]
    KafiMA,AktarMK,PhannyY,et al.Adhesion, proliferation and differentiation of human mesenchymal stem cell on chitosan/collagen composite scaffold[J].J Mater Sci Mater Med,2019,30(12):131.DOI: 10.1007/s10856-019-6341-8.
    [10]
    李伟,孔维诗,包郁露,等.负载脂肪干细胞的皮肤组织工程支架在创面修复中的研究进展[J].中华烧伤与创面修复杂志,2023,39(11):1090-1095.DOI: 10.3760/cma.j.cn501225-20221123-00502.
    [11]
    PrasathkumarM,SadhasivamS.Chitosan/hyaluronic acid/alginate and an assorted polymers loaded with honey, plant, and marine compounds for progressive wound healing-know-how[J].Int J Biol Macromol,2021,186:656-685.DOI: 10.1016/j.ijbiomac.2021.07.067.
    [12]
    LiQ,ZhaoH,ChenW,et al.Human keratinocyte-derived microvesicle miRNA-21 promotes skin wound healing in diabetic rats through facilitating fibroblast function and angiogenesis[J].Int J Biochem Cell Biol,2019,114:105570.DOI: 10.1016/j.biocel.2019.105570.
    [13]
    LiuY,LiuY,WuM,et al.Adipose-derived mesenchymal stem cell-loaded β-chitin nanofiber hydrogel promote wound healing in rats[J].J Mater Sci Mater Med,2022,33(2):12.DOI: 10.1007/s10856-021-06630-7.
    [14]
    AlanS,ŞalvaE,Yılmazİ,et al.The effectiveness of chitosan-mediated silencing of PDGF-B and PDGFR-β in the mesangial proliferative glomerulonephritis therapy[J].Exp Mol Pathol,2019,110:104280.DOI: 10.1016/j.yexmp.2019.104280.
    [15]
    SalehH,El-ShorbagyHM.Chitosan protects liver against ischemia-reperfusion injury via regulating Bcl-2/Bax, TNF-α and TGF-β expression[J].Int J Biol Macromol,2020,164:1565-1574.DOI: 10.1016/j.ijbiomac.2020.07.212.
    [16]
    van SteenbergheM,SchubertT,GuiotY,et al.Improvement of mesh recolonization in abdominal wall reconstruction with adipose vs. bone marrow mesenchymal stem cells in a rodent model[J].J Pediatr Surg,2017,52(8):1355-1362.DOI: 10.1016/j.jpedsurg.2016.11.041.
    [17]
    RavishankarK,VenkatesanM,DesinghRP,et al.Biocompatible hydrogels of chitosan-alkali lignin for potential wound healing applications[J].Mater Sci Eng C Mater Biol Appl,2019,102:447-457.DOI: 10.1016/j.msec.2019.04.038.
    [18]
    LiangY,ZhaoX,HuT,et al.Adhesive hemostatic conducting injectable composite hydrogels with sustained drug release and photothermal antibacterial activity to promote full-thickness skin regeneration during wound healing[J].Small,2019,15(12):e1900046.DOI: 10.1002/smll.201900046.
    [19]
    NiZ,YuH,WangL,et al.Polyphosphazene and non-catechol-based antibacterial injectable hydrogel for adhesion of wet tissues as wound dressing[J].Adv Healthc Mater,2022,11(1):e2101421.DOI: 10.1002/adhm.202101421.
    [20]
    DavisFM,GallagherKA.Epigenetic mechanisms in monocytes/macrophages regulate inflammation in cardiometabolic and vascular disease[J].Arterioscler Thromb Vasc Biol,2019,39(4):623-634.DOI: 10.1161/ATVBAHA.118.312135.
    [21]
    ShiC,YuanF,LiZ,et al.MSN@IL-4 sustainingly mediates macrophagocyte M2 polarization and relieves osteoblast damage via NF-κB pathway-associated apoptosis[J].Biomed Res Int,2022,2022:2898729.DOI: 10.1155/2022/2898729.
    [22]
    YangT,TanY,ZhangW,et al.Effects of ALA-PDT on the healing of mouse skin wounds infected with pseudomonas aeruginosa and its related mechanisms[J].Front Cell Dev Biol,2020,8:585132.DOI: 10.3389/fcell.2020.585132.
    • Relative Articles
    • Supplements(0)
    • Cited By
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(4)  / Tables(3)

    Get Citation
    PDF
    XML

    Article Metrics

    Article views (4611) PDF downloads(47) Cited by()
    Proportional views
    Related

    Copyright © Chinese Journal of Burns京ICP备07035254号-14

    E-mail：shaoshangzazhi@163.com

    Website：https://zhsszz.xml-journal.net/

    Supported by: Beijing Renhe Information Technology Co. Ltd    

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return