Shen Jinfu, Jiang Ruimei, Wang Zhuoqun, et al. Recurrence and influencing factors of diabetic foot ulcer in patients with type 2 diabetes mellitus[J]. Chin j Burns, 2020, 36(10): 947-952. Doi: 10.3760/cma.j.cn501120-20190726-00315
Citation: Cao T,Hao T,Xiao D,et al.Effect and mechanism of human adipose-derived stem cell exosomes on diabetic peripheral neuropathy[J].Chin J Burns Wounds,2024,40(3):240-248.DOI: 10.3760/cma.j.cn501225-20231207-00230.

Effect and mechanism of human adipose-derived stem cell exosomes on diabetic peripheral neuropathy

doi: 10.3760/cma.j.cn501225-20231207-00230
Funds:

General Program of National Natural Science Foundation of China 82272269

More Information
  •   Objective   To investigate the changes of artemin protein expression in diabetic peripheral neuropathy (DPN) and to explore the regulatory effect of human adipose-derived stem cell (ADSC) exosomes on the change of artemin protein expression.   Methods   This research was a prospective observational clinical research combined with experimental research. Thirteen DPN patients (9 males and 4 females, aged 32 to 68 years) who were admitted to the First Affiliated Hospital of Air Force Medical University (hereinafter referred to as our hospital) from May 2022 to October 2023 and met the inclusion criteria were selected as DPN group, and 5 non-diabetes patients (4 males and 1 female, aged 29 to 61 years) who were admitted to our hospital in the same period of time and met the inclusion criteria were selected as control group. The toe nerve or sural nerve tissue in the abandoned tissue after debridement or amputation of patients in the two groups was collected. The pathological changes of nerve tissue were observed after hematoxylin-eosin staining; the protein expressions of S100β and artemin in nerve tissue were observed after immunofluorescence staining, and the artemin protein expression was quantified; the protein and mRNA expressions of artemin were detected by Western blotting and real-time fluorescent quantitative reverse transcription polymerase chain reaction, respectively (the sample number in DPN group and control group was 13 and 5, respectively). Twelve male C57BL/6 mice aged 3 to 5 days were collected to isolate Schwann cells, and the cells were divided into conventional culture group cultured routinely, high glucose alone group (cultured with high concentration of glucose solution only), and high glucose+exosome group (cultured with high concentration of glucose solution and extracted human ADSC exosomes). After 24 hours of culture, the cell proliferation activity was detected by cell counting kit 8 ( n=6). After 48 hours of culture, the protein expression of artemin was detected by Western blotting ( n=3).   Results   Compared with those in control group, the neural supporting cells decreased and the inflammatory cells increased in the nerve tissue of patients in DPN group, showing typical manifestations of nerve injury. Immunofluorescence staining showed that compared with those in control group, the nuclei was more, and the protein expression of S100β was lower in nerve tissue of patients in DPN group. The protein expression of artemin in nerve tissue of patients in DPN group was 71±31, which was significantly lower than 1 729±62 in control group ( t=76.92, P<0.05). Western blotting detection showed that the protein expression of artemin in nerve tissue of patients in DPN group was 0.74±0.08, which was significantly lower than 0.97±0.06 in control group ( t=5.49, P<0.05). The artemin mRNA expression in nerve tissue of patients in DPN group was significantly lower than that in control group ( t=7.65, P<0.05). After 24 hours of culture, compared with that in conventional culture group, the proliferation activities of Schwann cells in high glucose alone group and high glucose+exosome group were significantly decreased ( P<0.05); compared with that in high glucose alone group, the proliferation activity of Schwann cells in high glucose+exosome group was significantly increased ( P<0.05). After 48 hours of culture, compared with those in conventional culture group, the protein expressions of artemin of Schwann cells in high glucose alone group and high glucose+exosome group were significantly decreased ( P<0.05); compared with that in high glucose alone group, the protein expression of artemin of Schwann cells in high glucose+exosome group was significantly increased ( P<0.05).   Conclusions   The protein expression of artemin in nerve tissue of DPN patients is lower than that in normal nerve tissue, which may be related to the reduction of proliferation activity of Schwann cells by high glucose. Human ADSC exosomes may improve the proliferation activity of Schwann cells by increasing artemin protein expression, thereby delaying the progression of DPN.

     

  • [1]
    RehmanZU.Saving limbs in diabetics: challenges and opportunities[J].J Coll Physicians Surg Pak,2020,30(10):1003-1004.DOI: 10.29271/jcpsp.2020.10.1003.
    [2]
    LowLL, KwanYH, KoMSM, et al. Epidemiologic characteristics of multimorbidity and sociodemographic factors associated with multimorbidity in a rapidly aging Asian country[J]. JAMA Netw Open, 2019,2(11):e1915245. DOI: 10.1001/jamanetworkopen.2019.15245.
    [3]
    《多学科合作下糖尿病足防治专家共识(2020版)》编写组.多学科合作下糖尿病足防治专家共识(2020版)全版[J].中华烧伤杂志,2020,36(8):E01-E52.DOI: 10.3760/cma.j.cn501120-20200217-01000.
    [4]
    Sable-MoritaS,OkuraM,TanikawaT,et al.Associations between diabetes-related foot disease, diabetes, and age-related complications in older patients[J].Eur Geriatr Med,2021,12(5):1003-1009.DOI: 10.1007/s41999-021-00491-7.
    [5]
    ElafrosMA,AndersenH,BennettDL,et al.Towards prevention of diabetic peripheral neuropathy: clinical presentation, pathogenesis, and new treatments[J].Lancet Neurol,2022,21(10):922-936.DOI: 10.1016/S1474-4422(22)00188-0.
    [6]
    EidSA,RumoraAE,BeirowskiB,et al.New perspectives in diabetic neuropathy[J].Neuron,2023,111(17):2623-2641.DOI: 10.1016/j.neuron.2023.05.003.
    [7]
    YinK,QiaoT,ZhangY,et al.Unraveling shared risk factors for diabetic foot ulcer: a comprehensive Mendelian randomization analysis[J].BMJ Open Diabetes Res Care,2023,11(6):e003523. DOI: 10.1136/bmjdrc-2023-003523.
    [8]
    SenCK,RoyS,KhannaS.Diabetic peripheral neuropathy associated with foot ulcer: one of a kind[J/OL].Antioxid Redox Signal,2023(2023-01-25)[2023-12-07]. https://pubmed.ncbi.nlm.nih.gov/35850520/.DOI:10.1089/ars.2022.0093.[published online ahead of print].
    [9]
    ChenD,WangM,ShangX,et al.Development and validation of an incidence risk prediction model for early foot ulcer in diabetes based on a high evidence systematic review and meta-analysis[J].Diabetes Res Clin Pract,2021,180:109040.DOI: 10.1016/j.diabres.2021.109040.
    [10]
    BhandariR,SharmaA,KuhadA.Novel nanotechnological approaches for targeting dorsal root ganglion (DRG) in mitigating diabetic neuropathic pain (DNP)[J].Front Endocrinol (Lausanne),2022,12:790747.DOI: 10.3389/fendo.2021.790747.
    [11]
    SloanG, AlamU, SelvarajahD, et al. The treatment of painful diabetic neuropathy [J]. Curr Diabetes Rev, 2022, 18(5): e070721194556. DOI: 10.2174/1573399817666210707112413.
    [12]
    NoceraG,JacobC.Mechanisms of Schwann cell plasticity involved in peripheral nerve repair after injury[J].Cell Mol Life Sci,2020,77(20):3977-3989.DOI: 10.1007/s00018-020-03516-9.
    [13]
    Bosch-QueraltM,FledrichR,StassartRM.Schwann cell functions in peripheral nerve development and repair[J].Neurobiol Dis,2023,176:105952.DOI: 10.1016/j.nbd.2022.105952.
    [14]
    RachanaKS,ManuMS,AdviraoGM.Insulin-induced upregulation of lipoprotein lipase in Schwann cells during diabetic peripheral neuropathy[J].Diabetes Metab Syndr,2018,12(4):525-530.DOI: 10.1016/j.dsx.2018.03.017.
    [15]
    ZhangX,ZhaoS,YuanQ,et al.TXNIP, a novel key factor to cause Schwann cell dysfunction in diabetic peripheral neuropathy, under the regulation of PI3K/Akt pathway inhibition-induced DNMT1 and DNMT3a overexpression[J].Cell Death Dis,2021,12(7):642.DOI: 10.1038/s41419-021-03930-2.
    [16]
    ZhuS,LiY,BennettS,et al.The role of glial cell line-derived neurotrophic factor family member artemin in neurological disorders and cancers[J].Cell Prolif,2020,53(7):e12860.DOI: 10.1111/cpr.12860.
    [17]
    IlievaM, NielsenJ, KorshunovaI, et al. Artemin and an Artemin-derived peptide, artefin, induce neuronal survival, and differentiation through ret and NCAM[J].Front Mol Neurosci, 2019,12:47. DOI: 10.3389/fnmol.2019.00047.
    [18]
    WangR,RossomandoA,SahDWY,et al.Artemin induced functional recovery and reinnervation after partial nerve injury[J].Pain,2014,155(3):476-484.DOI: 10.1016/j.pain.2013.11.007.
    [19]
    WongLE, GibsonME, ArnoldHM, et al. Artemin promotes functional long-distance axonal regeneration to the brainstem after dorsal root crush[J]. Proc Natl Acad Sci U S A, 112(19):6170-6175. DOI: 10.1073/pnas.1502057112.
    [20]
    中华医学会糖尿病学分会,中华医学会感染病学分会,中华医学会组织修复与再生分会.中国糖尿病足防治指南(2019版)(Ⅰ)[J].中华糖尿病杂志,2019,11(2):92-108.DOI: 10.3760/cma.j.issn.1674-5809.2019.02.004.
    [21]
    ZhangY,BiJ,HuangJ,et al.Exosome: a review of its classification, isolation techniques, storage, diagnostic and targeted therapy applications[J].Int J Nanomedicine,2020,15:6917-6934.DOI: 10.2147/IJN.S264498.
    [22]
    ShanF,JiQ,SongY,et al.A fast and efficient method for isolating Schwann cells from sciatic nerves of neonatal mice[J].J Neurosci Methods,2022,366:109404.DOI: 10.1016/j.jneumeth.2021.109404.
    [23]
    LiK,ShiX,LuoM,et al.Taurine protects against myelin damage of sciatic nerve in diabetic peripheral neuropathy rats by controlling apoptosis of Schwann cells via NGF/Akt/GSK3β pathway[J].Exp Cell Res,2019,383(2):111557.DOI: 10.1016/j.yexcr.2019.111557.
    [24]
    LiuB,XinW,TanJR,et al.Myelin sheath structure and regeneration in peripheral nerve injury repair[J].Proc Natl Acad Sci U S A,2019,116(44):22347-22352.DOI: 10.1073/pnas.1910292116.
    [25]
    AhmedZ,SuggateEL,BrownER,et al.Schwann cell-derived factor-induced modulation of the NgR/p75NTR/EGFR axis disinhibits axon growth through CNS myelin in vivo and in vitro[J].Brain,2006,129(Pt 6):1517-1533.DOI: 10.1093/brain/awl080.
    [26]
    HuangL,XiaB,ShiX,et al.Time-restricted release of multiple neurotrophic factors promotes axonal regeneration and functional recovery after peripheral nerve injury[J].FASEB J,2019,33(7):8600-8613.DOI: 10.1096/fj.201802065RR.
    [27]
    MajdH,AminS,GhazizadehZ,et al.Deriving Schwann cells from hPSCs enables disease modeling and drug discovery for diabetic peripheral neuropathy[J].Cell Stem Cell,2023,30(5):632-647.e10.DOI: 10.1016/j.stem.2023.04.006.
    [28]
    YuanQ,ZhangX,WeiW,et al.Lycorine improves peripheral nerve function by promoting Schwann cell autophagy via AMPK pathway activation and MMP9 downregulation in diabetic peripheral neuropathy[J].Pharmacol Res,2022,175:105985.DOI: 10.1016/j.phrs.2021.105985.
    [29]
    BolonB,JingS,AsuncionF,et al.The candidate neuroprotective agent artemin induces autonomic neural dysplasia without preventing peripheral nerve dysfunction[J].Toxicol Pathol,2004,32(3):275-294.DOI: 10.1080/01926230490431475.
    [30]
    TakakuS,TsukamotoM,NiimiN,et al.Exendin-4 promotes Schwann cell survival/migration and myelination in vitro[J].Int J Mol Sci,2021,22(6):2971.DOI: 10.3390/ijms22062971.
    [31]
    YinY,QuH,YangQ,et al.Astragaloside Ⅳ alleviates Schwann cell injury in diabetic peripheral neuropathy by regulating microRNA-155-mediated autophagy[J].Phytomedicine,2021,92:153749.DOI: 10.1016/j.phymed.2021.153749.
    [32]
    FontanaX,HristovaM,Da CostaC,et al.c-Jun in Schwann cells promotes axonal regeneration and motoneuron survival via paracrine signaling[J].J Cell Biol,2012,198(1):127-141.DOI: 10.1083/jcb.201205025.
    [33]
    de AssisACC,ReisALS,NunesLV,et al.Stem cells and tissue engineering-based therapeutic interventions: promising strategies to improve peripheral nerve regeneration[J].Cell Mol Neurobiol,2023,43(2):433-454.DOI: 10.1007/s10571-022-01199-3.
    [34]
    RhodeSC,BeierJP,RuhlT.Adipose tissue stem cells in peripheral nerve regeneration-in vitro and in vivo[J].J Neurosci Res,2021,99(2):545-560.DOI: 10.1002/jnr.24738.
    [35]
    AzamM,GhufranH,ButtH,et al.Curcumin preconditioning enhances the efficacy of adipose-derived mesenchymal stem cells to accelerate healing of burn wounds[J/OL].Burns Trauma,2021,9:tkab021[2023-12-07].https://pubmed.ncbi.nlm.nih.gov/34514007/.DOI: 10.1093/burnst/tkab021.
    [36]
    FanB, LiC, SzaladA, et al. Mesenchymal stromal cell-derived exosomes ameliorate peripheral neuropathy in a mouse model of diabetes[J]. Diabetologia, 2020, 63(2): 431-443.DOI: 10.1007/s00125-019-05043-0.
    [37]
    YangZ,YangY,XuY,et al.Biomimetic nerve guidance conduit containing engineered exosomes of adipose-derived stem cells promotes peripheral nerve regeneration[J].Stem Cell Res Ther,2021,12(1):442.DOI: 10.1186/s13287-021-02528-x.
    [38]
    YinG, YuB, LiuC, et al. Exosomes produced by adipose-derived stem cells inhibit schwann cells autophagy and promote the regeneration of the myelin sheath [J]. Int J Biochem Cell Biol, 2021, 132:105921. DOI: 10.1016/j.biocel.2021.105921.
  • Relative Articles

    [1]Zhao Jianjun, Xie Zhenjun, Zhao Guohong, Zhang Jianhua, Sun Huawei, Bai Huikai, Zhang Huifeng, Zhang Dongbin, Xiao Erhui, Zhu Guosong. Clinical effects of free anterolateral thigh perforator flaps in repairing diabetic foot ulcers under a multi-disciplinary team cooperation model[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2024, 40(8): 756-761. doi: 10.3760/cma.j.cn501225-20231107-00184
    [2]Liu Xin, Huang Guangtao, Wu Jun. Research advances on the application of free flaps in limb salvage treatment of patients with diabetic foot ulcers[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2024, 40(10): 985-990. doi: 10.3760/cma.j.cn501225-20240130-00041
    [3]Chen Wei, Chang Shusen, Zhou Jian, Zhang Fang, Yang Chenglan, Nie Kaiyu, Deng Chengliang, Wei Zairong. Clinical effects of antibiotic bone cement combined with free anterolateral thigh flap in sequential treatment of diabetic foot ulcer[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2023, 39(4): 319-324. doi: 10.3760/cma.j.cn501225-20220628-00267
    [4]Jian Yang, Wei Zairong, Chen Wei, Zhang Yanji, Tang Mingyuan, Zhong Yunxue, Liu Chenxiaoxiao. Research advances on the application of free flaps in repairing diabetic foot ulcers[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2023, 39(4): 376-380. doi: 10.3760/cma.j.cn501225-20221216-00539
    [5]Wang Qian, Zhu Hongjuan, Feng Ying, Chu Wanli, Song Yaoyao. A cross-sectional survey and analysis of the pain status and its influencing factors in diabetic foot ulcer patients[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2023, 39(4): 330-336. doi: 10.3760/cma.j.cn501225-20220421-00150
    [6]Guo Qingjiao, Ouyang Jing, Rao Jiaqin, Zhang Yizhi, Yu Lihong, Xu Wanying, Long Jinhua, Gao Xiuhua, Wu Xiaoyan, Gu Ying. Construction and preliminary validation of a risk prediction model for the recurrence of diabetic foot ulcer in diabetic patients[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2023, 39(12): 1149-1157. doi: 10.3760/cma.j.cn501225-20231101-00166
    [7]Wang Ning, Ju Shang. Research advances on the mechanism of refractory healing of diabetic foot ulcer[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2022, 38(11): 1085-1089. doi: 10.3760/cma.j.cn501225-20220227-00038
    [8]Wang Peng, Chen Zhaohong, Jiang Liyuan, Zhou Xiaoqian, Jia Chiyu, Xiao Hou'an. Screening, functional analysis and clinical validation of differentially expressed genes in diabetic foot ulcers[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2022, 38(10): 944-951. doi: 10.3760/cma.j.cn501225-20220731-00328
    [9]Research progress of induced pluripotent stem cells in promoting wound healing of diabetic foot ulcers[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2022, 38(9): 864-869. doi: 10.3760/cma.j.cn501120-20210630-00230
    [10]Yu Xiaoyuan, Zhao Mingyu, Zhang Ying, Xu Gang. Research advances on the treatment of diabetic foot ulcers with autologous platelet-rich fibrin[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2022, 38(12): 1185-1189. doi: 10.3760/cma.j.cn501225-20220110-00001
    [11]Tissue Repair of Burns and Trauma Committee, Cross-Straits Medicine Exchange Association of China. National expert consensus on the application of negative pressure wound therapy in the treatment of diabetic foot wounds (2021 version)[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2021, 37(6): 508-518. doi: 10.3760/cma.j.cn501120-20210107-00010
    [12]Li Feifei, Zhang Jie, Gao Chunchen, Wang Tianyuan, Zhi Liming, Wang Jing, Wang Aiping. Influence of maggot excretions/secretions on the anti-Pseudomonas aeruginosa effect of neutrophils in patients with diabetic foot ulcer[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2021, 37(5): 413-419. doi: 10.3760/cma.j.cn501120-20210312-00085
    [13]Zhao Peng, Yang Minlie, Chu Guoping, Jia Zhigang, Zhou Xiaojin, Lyu Guozhong. Influence of porcine urinary bladder matrix and porcine acellular dermal matrix on wound healing of full-thickness skin defect in diabetic mice[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2020, 36(12): 1130-1138. doi: 10.3760/cma.j.cn501120-20200901-00399
    [14]Shao Jiaming, Wang Xingang, Yu Chaoheng, Han Chunmao. Teicoplanin-induced hypersensitivity syndrome in a diabetic foot patient with malignant ulcer[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2020, 36(8): 747-750. doi: 10.3760/cma.j.cn501120-20190617-00273
    [15]Huang Hongjun, Niu Xihua, Yang Guanlong, Wang Liying, Shi Fanchao, Xu Shaojun, Xu Lingang, Li Yonglin. Clinical effects of application of antibiotic bone cement in wounds of diabetic foot ulcers[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2019, 35(6): 464-466. doi: 10.3760/cma.j.issn.1009-2587.2019.06.013
    [17]Liu Xiaolong, Su Fuzeng, Zha Tianjian, Liu Lihua, Wang Zhizhong, Nuerlan null. Analysis on the risk factors of diabetic foot ulcer in diabetic patients of Uyghur nationality and Han nationality in the Xinjiang Uygur Autonomous Region[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2017, 33(8): 486-490. doi: 10.3760/cma.j.issn.1009-2587.2017.08.006
    [18]Xue Chunli, Hu Zhicheng, Yang Zuxian, Li Xiaojian. Meta-analysis on the clinical effects of allogenic acellular dermal matrix treatment for diabetic foot ulcer[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2016, 32(12): 725-729. doi: 10.3760/cma.j.issn.1009-2587.2016.12.005
    [20]ZHANG Zhao-xin, LIU Xiao-long, LU Lei, ZHANG Liang, JI Dong-liang, LIU Li-hua. Effect of insulin by local injection on the level of systemic blood glucose and granulation tissue formation of wound in patients with diabetic foot ulcer[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2011, 27(6): 451-455. doi: 10.3760/cma.j.issn.1009-2587.2011.06.014
  • Cited by

    Periodical cited type(20)

    1. 冯秀丽,赵仁豪,杨腾,王娜,王国凤. 麻雀搜索算法优化的BP神经网络模型对糖尿病足的预测价值. 江苏医药. 2025(01): 37-42+117 .
    2. 周伟,郑红波,刘强,倪小坤,韦旻兴. 糖尿病足患者溃疡愈合的影响因素以及中西医治疗的研究进展. 糖尿病新世界. 2024(01): 195-198 .
    3. 吴翩,郑晖,陈功雷. 小野寺预后营养指数与糖尿病溃疡足严重程度关系及对预后的预测作用. 医学研究与战创伤救治. 2024(01): 72-76 .
    4. 文博,王汐玉,燕辛,莫然,林樾,谭谦. 糖尿病足复发风险预测模型的构建:联合时间依赖ROC的生存分析. 中国美容医学. 2024(05): 26-31 .
    5. 鹿艳军. 糖尿病足溃疡患者愈合后复发的影响因素. 中国民康医学. 2024(16): 6-9 .
    6. 万星宇,夏磊,庄若,朱丽群,眭升,梁臣,张炜. 糖尿病足溃疡复发相关预防措施的系统评价再评价. 中华现代护理杂志. 2024(27): 3647-3657 .
    7. 王红,李亮. 糖尿病足患者溃疡愈合后复发的影响因素及风险模型构建. 中国实用医刊. 2023(01): 54-58 .
    8. 耿怡丹,胡佳琪,董雪凡,李阳,田建丽. 糖尿病足溃疡病人复发危险因素的Meta分析. 全科护理. 2023(10): 1325-1328 .
    9. 夏磊,孙金姗,庄若,梁臣. 复发性糖尿病足溃疡相关研究的文献计量学分析. 全科护理. 2023(18): 2458-2462 .
    10. 冉倩,田娇,赵锡丽. 糖尿病足发病风险预测模型的研究进展. 重庆医学. 2023(15): 2374-2378 .
    11. 郭庆娇,欧阳静,饶佳琴,张艺之,余丽红,徐婉莹,龙锦花,高秀花,吴晓艳,顾颖. 糖尿病患者糖尿病足溃疡复发风险预测模型的构建及初步验证. 中华烧伤与创面修复杂志. 2023(12): 1149-1157 . 本站查看
    12. 吕静,袁丽,李饶,黄凤梅. 糖尿病足溃疡复发风险预测模型的构建. 护理研究. 2022(06): 993-998 .
    13. 夏磊,庄若,吴玲,魏敏. 复发性糖尿病足溃疡相关危险因素的Meta分析. 中华现代护理杂志. 2022(09): 1143-1148 .
    14. 王沆,雷云宏,严骏,林君垚,林隆辉,黄烽. DSA下介入手术治疗对糖尿病足患者溃疡复发率及疼痛程度的影响. 糖尿病新世界. 2022(15): 184-186+190 .
    15. 王鹏,陈昭宏,蒋丽媛,周小茜,贾赤宇,肖厚安. 糖尿病足溃疡差异表达基因的筛选与功能分析及临床验证. 中华烧伤与创面修复杂志. 2022(10): 944-951 . 本站查看
    16. 曾诗叶,姚志远,段欣,黄金. 糖尿病足患者就诊延迟的研究现状. 中华糖尿病杂志. 2022(11): 1332-1336 .
    17. 李温温,陈啸,刘亚萍,王琳茹,王晋军,黄贤明. 糖尿病足复发感染患者热休克蛋白70基因多态性. 中华医院感染学杂志. 2021(14): 2081-2084 .
    18. 王小玲,姜丽萍,姚文,李雯,唐雯婷,陈丽,王帆,陈寒蓓,简蔚霞,杨震. 中文版糖尿病足风险筛查及分级工具预测效果检验. 护理学杂志. 2021(17): 13-16 .
    19. 丘玉文,黎丽萍. 糖尿病肾病、糖尿病周围神经病变与糖化白蛋白的相关性分析. 糖尿病新世界. 2021(15): 35-37+45 .
    20. 陈思含,张云秋. 基于机器学习的2型糖尿病并发症预测模型研究. 中华医学图书情报杂志. 2020(11): 31-38 .

    Other cited types(13)

  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(7)

    Article Metrics

    Article views (3227) PDF downloads(104) Cited by(33)
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return