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Article Navigation >  CHINESE JOURNAL OF BURNS AND WOUNDS  >  > Accepted Manuscript
Zhu Yimeng,Jin Jian,Wang Weiwei.Performance of PHMB-surface mesoporous silica composite material and its effect on full-thickness skin defect infected wounds in rats[J].Chin J Burns Wounds,2026,42(7):1-10.DOI: 10.3760/cma.j.cn501225-20250830-00373.
Citation: Zhu Yimeng,Jin Jian,Wang Weiwei.Performance of PHMB-surface mesoporous silica composite material and its effect on full-thickness skin defect infected wounds in rats[J].Chin J Burns Wounds,2026,42(7):1-10.DOI: 10.3760/cma.j.cn501225-20250830-00373.
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Performance of PHMB-surface mesoporous silica composite material and its effect on full-thickness skin defect infected wounds in rats

doi: 10.3760/cma.j.cn501225-20250830-00373
  • 1.

    Department of Burn and Plastic, Shanghai Children's Medical Center Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China

  • 2.

    Department of Polymer Science, Fudan University, Shanghai 200433, China

  • 3.

    Department of Anesthesiology, the First Affiliated Hospital of Naval Medical University, Shanghai 200433, China

Funds:

National Key Research and Development Program of China 2024YFC2420800

Shanghai "Science and Technology Innovation Action Plan" Rising Star Talent Project 23QB1401000

More Information
  • Corresponding author: Wang Weiwei, Email: chzks6768@163.com
  • Received Date: 2025-08-30
    Available Online: 2026-07-03
    Abstract
  •   Objective  To investigate the performance of polyhexamethylene biguanide hydrochloride (PHMB)-surface mesoporous silica composite material (hereinafter referred to as the composite material) and its effect on full-thickness skin defect infected wounds in rats.  Methods  This study was an experimental study including group design, factorial design, and repeated measures design. The composite material with good biocompatibility was prepared by rotary evaporation method. The in vitro release of PHMB from the composite material was detected by continuous quantitative release assay. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of PHMB and the composite material against Staphylococcus aureus and Escherichia coli were detected by broth microdilution method. The continuous bactericidal duration of PHMB and the composite material was detected by continuous bacterial challenge assay. The inducing effect of PHMB and the composite material on the resistance of Staphylococcus aureus and Escherichia coli was detected by sub-lethal concentration induction assay. The sample size for the above experiments was 3. Forty-five female SD rats aged 10-12 weeks were divided into routine dressing change group, PHMB group, and composite material group by random number table method, with 15 rats in each group. After preparing full-thickness skin defect infected wounds on the back of all rats, the wounds of rats in routine dressing change group, PHMB group, and composite material group were rinsed and dressed with normal saline, PHMB solution and composite material dispersion with a final mass fraction of 0.100% PHMB, respectively. The wound healing rate and infection status were calculated at post injury day (PID) 6, 12, and 18 with sample sizes of 15, 10, and 5, respectively. At PID 6, 12, and 18, 5 rats from each group were selected by random number table method before dressing change, and the wound tissue bacterial load was detected by viable plate count method.  Results  The composite material with a mass ratio of PHMB to surface mesoporous silica of 1:1 could continuously release PHMB for ≥ 144 h. For both Staphylococcus aureus and Escherichia coli, the MICs of the composite material were lower than those of PHMB, but the MBCs of both materials were identical. The continuous bactericidal duration of PHMB against Staphylococcus aureus and Escherichia coli was both 3 d, and the continuous bactericidal duration of the composite material against Staphylococcus aureus and Escherichia coli was both 6 d. Staphylococcus aureus and Escherichia coli did not show resistance to the composite material after 60 d of culture, which was significantly later than the resistance to PHMB. The wound healing rates of rats in composite material group at PID 6, 12, and 18 were significantly higher than those in routine dressing change group and PHMB group (P<0.05). At PID 6, 12, and 18, no infection occurred in the wounds of rats in composite material group, whereas all wounds in routine dressing change group continuously showed infection, and some wounds in PHMB group still continuously showed infection. The wound tissue bacterial loads of rats in composite material group at PID 6, 12, and 18 were (4.6±3.1), (3.4±3.4), and (1.6±1.5) CFU/mL, respectively, which were significantly lower than those in routine dressing change group (( 1876.0±241.5), (1 076.0±151.1), and (1 184.0±156.6) CFU/mL) and PHMB group ((824.0±277.6), (800.0±231.0), and (832.0±350.9) CFU/mL, P<0.05). The wound tissue bacterial load of rats in PHMB group at PID 6 and 12 was significantly lower than that in routine dressing change group (P<0.05).  Conclusions  The composite material has the activities of synergistic effect and sustained release of PHMB, thereby delaying the generation of bacterial resistance, reducing the bacterial load of wound tissue, and facilitating wound healing.

     

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    • References(38)
  • [1]
    JinJ, PengY, ChenZL,et al. Determining transfusion use in major burn patients: a retrospective review and analysis from 2009 to 2019[J].Burns,2022,48(5):1104-1111.DOI: 10.1016/j.burns.2021.09.004.
    [2]
    HuX,WangX,HongX,et al.Modification and utility of a rat burn wound model[J].Wound Repair Regen,2020,28(6):797-811.DOI: 10.1111/wrr.12855.
    [3]
    杨惠忠,徐正鹏,原博,等.自体表皮细胞悬液与网状自体刃厚皮移植联合治疗深Ⅱ度烧伤创面的临床研究[J].组织工程与重建外科杂志,2024,20(4):411-415.DOI: 10.3969/j.issn.1673-0364.2024.04.003.
    [4]
    傅聪颖,黎宁,李茂君,等.非药物干预治疗患者慢性创面疼痛效果的系统评价与贝叶斯网状荟萃分析[J].中华烧伤与创面修复杂志,2025,41(5):491-500.DOI: 10.3760/cma.j.cn501225-20241213-00486.
    [5]
    JinJ, TangT, ZhouH, et al. Synergistic effects of quercetin-modified silicone gel sheet in scar treatment[J]. J Burn Care Res, 2022, 43(2): 445-452. DOI: 10.1093/jbcr/irab100.
    [6]
    邢楠, 霍然, 王海涛, 等. 脂肪干细胞基质胶促进创面愈合的研究进展[J].中华烧伤与创面修复杂志, 2023, 39(1): 81-84. DOI: 10.3760/cma.j.cn501120-20211204-00404.
    [7]
    SatoH,NakaiY,SudaH,et al.A shaped pectoralis major muscle flap under indocyanine green fluorescence angiography for sternal wound infection[J].Plast Reconstr Surg Glob Open,2024,12(6):e5876.DOI: 10.1097/GOX.0000000000005876.
    [8]
    LiS,LuM,DaiC,et al.Advanced palladium nanosheet-enhanced phototherapy for treating wound infection caused by multidrug-resistant bacteria[J].Small,2025,21(6):e2407180.DOI: 10.1002/smll.202407180.
    [9]
    蔡悦,程兴,詹剑华,等.烧伤后侵袭性真菌感染的研究进展[J].中华烧伤与创面修复杂志,2023,39(3):269-274.DOI: 10.3760/cma.j.cn501225-20220523-00199.
    [10]
    陈仲毅, 胡时强, 刘德伍, 等. 严重烧伤感染与细胞因子风暴的研究进展[J]. 中华烧伤与创面修复杂志, 2023, 39(4): 391-395. DOI: 10.3760/cma.j.cn501225-20220412-00134.
    [11]
    NakanishiR,OzawaH,ToyotaN,et al.Effectiveness of negative pressure wound therapy (NPWT) in preventing incisional surgical site infection after stoma closure: a single institutional retrospective study[J].Surg Today,2025,55(5):646-651.DOI: 10.1007/s00595-024-02983-y.
    [12]
    邓雅萍,邓呈亮.负压伤口疗法在游离皮瓣移植手术中的应用研究进展[J].中华烧伤与创面修复杂志,2024,40(9):885-890.DOI: 10.3760/cma.j.cn501225-20231106-00181.
    [13]
    HuC,HouB,YangF,et al.Enhancing diabetic wound healing through anti-bacterial and promoting angiogenesis using dual-functional slow-release microspheres-loaded dermal scaffolds[J].Colloids Surf B Biointerfaces,2024,242:114095.DOI: 10.1016/j.colsurfb.2024.114095.
    [14]
    RomanelliM,DiniV,PolignanoR,et al.Ibuprofen slow-release foam dressing reduces wound pain in painful exuding wounds: preliminary findings from an international real-life study[J].J Dermatolog Treat,2009,20(1):19-26.DOI: 10.1080/09546630802178232.
    [15]
    金荣华,张珍珍,徐鹏钦,等.三维生物打印抗菌型水凝胶对大鼠全层皮肤缺损创面的作用[J].中华烧伤与创面修复杂志,2023,39(2):165-174.DOI: 10.3760/cma.j.cn501120-20210809-00274.
    [16]
    夏欢,潘在兴,洪云,等.海口地区耐药结核病的流行病学特征及其影响因素分析[J].解放军医学杂志,2024,49(8):973-976.DOI: 10.11855/j.issn.0577-7402.1018.2024.0407.
    [17]
    FojaS,HeinzelmannJ,ViestenzA,et al.Evaluation of the possible influence of povidone iodine (PVI) solution and polyhexanide (PHMB) on wound healing in corneal epithelial regeneration[J].J Clin Med,2024,13(2):588.DOI: 10.3390/jcm13020588.
    [18]
    王智群,张晓玉,张阳,等.双氢青蒿素对体外培养兔角膜上皮细胞毒性作用的实验研究[J].眼科,2017,26(3):159-162.DOI: 10.13281/j.cnki.issn.1004-4469.2017.03.004.
    [19]
    刘聪颖,胡建华,杨东,等.多重响应性介孔二氧化硅纳米微球的制备及载药研究[J].化学学报,2009,67(8):843-849.DOI: 10.3321/j.issn:0567-7351.2009.08.022.
    [20]
    ZhaoD,FengJ,HuoQ,et al.Triblock copolymer syntheses of mesoporous silica with periodic 50 to 300 angstrom pores[J].Science,1998,279(5350):548-552.DOI: 10.1126/science.279.5350.548.
    [21]
    JowkarZ,MoaddeliA,ShafieiF,et al.Synthesis and characterization of mesoporous zinc oxide nanoparticles and evaluation of their biocompatibility in L929 fibroblasts[J].Clin Exp Dent Res,2024,10(1):e844.DOI: 10.1002/cre2.844.
    [22]
    李放, 陈蓓, 徐燕, 等. 消毒剂中PHMB和PHMG的测定方法探讨[J].环境监测管理与技术, 2018, 30(6): 54-56. DOI: 10.3969/j.issn.1006-2009.2018.06.013.
    [23]
    杨晓芳, 奚廷斐. 生物材料生物相容性评价研究进展[J]. 生物医学工程学杂志, 2001, 18(1): 123-128. DOI: 10.3321/j.issn:1001-5515.2001.01.033.
    [24]
    孟纯阳,安洪,蒋电明,等.纳米羟基磷灰石/聚酰胺的细胞相容性研究[J].中华创伤骨科杂志,2005,7(8):749-752.DOI: 10.3760/cma.j.issn.1671-7600.2005.08.015.
    [25]
    金剑,周浩,崔真慈,等.复合溶葡萄球菌酶消毒剂的抗菌作用及其对全层皮肤缺损大鼠移植人工真皮后感染的预防作用[J].中华烧伤杂志,2018,34(4):225-232.DOI: 10.3760/cma.j.issn.1009-2587.2018.04.007.
    [26]
    WangXQ, LiuPY, KempfM, et al. Burn wound infection in a porcine burn model[J]. J Burn Care Res, 2009, 30(2): 369-370. DOI: 10.1097/BCR.0b013e318198a757.
    [27]
    张锦丽,刘淑华,王德运,等.脱细胞异体真皮联合自体刃厚皮片移植修复小儿头面颈躯深度烧伤创面的效果[J].中华烧伤与创面修复杂志,2025,41(6):569-576.DOI: 10.3760/cma.j.cn501225-20240615-00231.
    [28]
    MihalacheA, TaoBK, HuangRS, et al. Chlorhexidine versus povidone-iodine for intravitreal injection antisepsis: a systematic review and meta-analysis[J]. Am J Ophthalmol, 2025, 276: 64-77. DOI: 10.1016/j.ajo.2025.03.031.
    [29]
    MolendijkMM, BoekemaBKHL, LattweinKR, et al.Bacteriophage therapy reduces Staphylococcus aureus in a porcine and human ex vivo burn wound infection model[J].Antimicrob Agents Chemother,2024,68(9):e0065024.DOI: 10.1128/aac.00650-24.
    [30]
    刘毅.提高感染性创面诊治水平需要关注的几个问题[J].中华烧伤与创面修复杂志,2024,40(2):119-124.DOI: 10.3760/cma.j.cn501225-20231018-00119.
    [31]
    HeS, HeW, ShiH, et al. Sono-piezodynamic therapy for drug-resistant bacteria infection[J]. Cell Rep Phys Sci, 2025,6(3): 102451. DOI: 10.1016/j.xcrp.2025.102451.
    [32]
    张卫红,陆明珠,邹凯,等.共缩聚法制备TEA-MCM-41介孔材料及其催化性能研究[J].化工新型材料,2024,52(3):203-208,213.DOI: 10.19817/j.cnki.issn1006-3536.2024.03.007.
    [33]
    WangB, WuX, JiaS, et al. Ultrahigh specific surface area mesoporous perovskite oxide nanosheets with rare-earth-enhanced lattice oxygen participation for superior water oxidation[J]. J Mater Sci Technol, 2025, 227: 255-261. DOI: 10.1016/j.jmst.2024.11.069.
    [34]
    KatsiotisCS,ÅhlénM,StrømmeM,et al.3D-printed mesoporous carrier system for delivery of poorly soluble drugs[J].Pharmaceutics,2021,13(7):1096.DOI: 10.3390/pharmaceutics13071096.
    [35]
    EgzaTF,KebedeEM,PerumalRD.Advancing malaria treatment with magnetic and mesoporous silica nanoparticles loaded with sulfadoxine-pyrimethamine: a review[J].Discov Nano,2025,20(1):199.DOI: 10.1186/s11671-025-04311-w.
    [36]
    王毛毛,张庆,吴博文,等.脂质体凝胶负载油酸促进慢性烧伤创面的修复[J].中国组织工程研究,2024,28(22):3524-3531.DOI: 10.12307/2024.524.
    [37]
    MoritzM, Geszke-MoritzM. Mesoporous materials as elements of modern drug delivery systems for anti-inflammatory agents: a review of recent achievements[J]. Pharmaceutics, 2022, 14(8): 1542. DOI: 10.3390/pharmaceutics14081542.
    [38]
    张薇,曾燕,汤雨佳.聚六亚甲基双胍抗菌敷料在肠造口患者放化疗期间造口周围皮肤溃疡中的应用效果[J].实用临床医药杂志,2024,28(7):129-132,137.DOI: 10.7619/jcmp.20233928.
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