Email Alerts
RSS
Turn off MathJax
Article Contents
Article Navigation >  CHINESE JOURNAL OF BURNS AND WOUNDS  >  > Accepted Manuscript
Shang Yijiang,Chen Yuerong,Liang Zhen,et al.Performance of phenylboronic acid-modified GelMA hydrogel loaded with copper-doped cerium oxide nanozyme and its effect on healing of infected burn wounds in mice[J].Chin J Burns Wounds,2026,42(8):1-10.DOI: 10.3760/cma.j.cn501225-20260430-00178.
Citation: Shang Yijiang,Chen Yuerong,Liang Zhen,et al.Performance of phenylboronic acid-modified GelMA hydrogel loaded with copper-doped cerium oxide nanozyme and its effect on healing of infected burn wounds in mice[J].Chin J Burns Wounds,2026,42(8):1-10.DOI: 10.3760/cma.j.cn501225-20260430-00178.
shu

Performance of phenylboronic acid-modified GelMA hydrogel loaded with copper-doped cerium oxide nanozyme and its effect on healing of infected burn wounds in mice

doi: 10.3760/cma.j.cn501225-20260430-00178
  • 1.

    School of Art and Design, Xi'an University of Technology, Xi'an 710048, China

  • 2.

    Department of Plastic Surgery, the First Affiliated Hospital of Air Military Medical University, Xi'an 710032, China

Funds:

Supporting Fund for the Shaanxi Province Young Outstanding Talent Support Program 106-451420001

More Information
    Abstract
  •   Objective  To explore the performance of phenylboronic acid-modified gelatin methacryloyl (GelMA) hydrogel loaded with copper-doped cerium oxide nanozyme (hereinafter referred to as composite hydrogel) and its effects on healing of infected burn wounds in mice.  Methods  This study was an experimental study including group design and repeated measures design. The phenylboronic acid-modified GelMA hydrogel (hereinafter referred to as simple hydrogel) with a three-dimensional loose porous network structure was prepared. The copper-doped cerium oxide nanozyme with a uniform spherical structure was prepared by a hydrothermal method. The copper-doped cerium oxide nanozyme with a final mass concentration of 100 μg/mL, which had no obvious effect on the viability of human umbilical vein endothelial cells and possessed high superoxide dismutase and catalase activities, was screened out and added into the simple hydrogel to prepare the composite hydrogel. After the composite hydrogels were soaked in phosphate buffer saline (PBS) or hydrogen peroxide solution for 24, 48, and 72 h, the release percentages of copper-doped cerium oxide nanozymes in the composite hydrogel were calculated, with sample size of 3. Thirty 8-week-old male BALB/c mice were taken, and after two burn wounds were created at symmetrical positions on both sides of the spine, methicillin-resistant Staphylococcus aureus (MRSA) bacterial suspension was dropped to construct infected burn wounds. According to the random number table method (the same grouping method below), the mice were divided into control group, simple hydrogel group, and composite hydrogel group, with 10 mice in each group (20 wounds). No drug intervention was performed on the wounds of the mice in control group, the simple hydrogel and composite hydrogel were dropped on the wounds of the mice in simple hydrogel group and composite hydrogel group, respectively, lasting for 14 d. At post injury day (PID) 3, 7, and 14, the percentage of residual wound area was measured and calculated, with sample size of 4. At PID 3, the MRSA concentration in the wound was calculated using the "plate counting method", with sample size of 3. At PID 5, the wound blood perfusion volume was evaluated by a laser speckle blood flow imaging system, with sample size of 3. Another 6 8-week-old male BALB/c mice were taken and divided into PBS group and composite hydrogel implantation group, with 3 mice in each group, and PBS was subcutaneously injected and composite hydrogels were subcutaneously implanted into the center of the mice's backs, respectively. At 14 d after treatment, abdominal whole blood was collected, then the blood routine test indexes and blood biochemical test indexes were detected.  Results  After immersion in PBS and hydrogen peroxide solution for 24, 48, and 72 h, respectively, the overall differences in the release percentages of copper-doped cerium oxide nanozymes in the composite hydrogel were statistically significant (with F values of 30.29 and 65.16, respectively, P<0.05). At PID 3, 7, and 14, compared with those in control group, the percentages of residual wound area of mice in simple hydrogel group and composite hydrogel group were all significantly reduced (P<0.05); the percentages of residual wound area of mice in composite hydrogel group were significantly lower than those in simple hydrogel group (P<0.05). At PID 3, compared with that in control group, the MRSA concentrations in the wounds of mice in simple hydrogel group and composite hydrogel group were both significantly decreased (P<0.05); the MRSA concentration in the wounds of mice in composite hydrogel group was significantly lower than that in simple hydrogel group (P<0.05). At PID 5, compared with (221±25) perfusion units in control group, the wound blood perfusion volumes of mice in simple hydrogel group and composite hydrogel group ((313±53) and (535±104) perfusion units, respectively) were both significantly increased (P<0.05); the wound blood perfusion volume of mice in composite hydrogel group was significantly higher than that in simple hydrogel group (P<0.05). At 14 d after treatment, the blood routine test indexes and blood biochemical test indexes of mice in composite hydrogel implantation group and PBS group were all maintained within normal physiological ranges. There were no statistically significant differences between the two groups of mice (P>0.05).  Conclusions  The composite hydrogel possesses good biocompatibility, reactive oxygen species responsiveness, and excellent antibacterial properties, and the healing of infected burn wounds in mice can be accelerated by it through the early elimination of MRSA in the wounds and the promotion of blood flow recovery.

     

    • FullText(HTML)
  • loading
    • References(38)
  • [1]
    MaJ, LiS, ZhangL, et al. Oxidativestress-scavenging thermo-activated MXene hydrogel for rapid repair of MRSA impaired wounds and burn wounds[J]. Mater Today, 2024, 80: 139-155. DOI: 10.1016/j.mattod.2024.08.010.
    [2]
    XuM, KhanA, WangT, et al. Mussel-inspired hydrogel with potent in vivo contact-active antimicrobial and wound healing promoting activities[J]. ACS Appl Bio Mater, 2019, 2(8): 3329-3340. DOI: 10.1021/acsabm.9b00353.
    [3]
    LiM, HouL, ZhangZ, et al. Pomegranate peel extracellular vesicle-incorporated GelMA hydrogel targets Staphylococcus aureus infection and accelerates wound healing[J]. ACS Appl Bio Mater, 2026, 9(3): 1522-1532. DOI: 10.1021/acsabm.5c02091.
    [4]
    LiuY,YangQ,ZhangL,et al.An ROS-responsive antioxidant hydrogel with immunomodulatory activity for promoting diabetic wound healing[J].Biomater Sci,2026,14(5):1292-1308.DOI: 10.1039/d6bm00018e.
    [5]
    ZhuZ,DingJ,QinM,et al.Enhanced ·OH-scavenging activity of Cu-CeOx nanozyme via resurrecting macrophage Nrf2 transcriptional activity facilitates diabetic wound healing[J].Adv Healthc Mater,2024,13(12):e2303229.DOI: 10.1002/adhm.202303229.
    [6]
    LiX,LiangH,HuangY,et al.Near-infrared light-responsive copper-cerium bimetallic oxide nanozyme with antibacterial and antioxidant abilities for periodontitis therapy[J].Colloids Surf B Biointerfaces,2025,252:114685.DOI: 10.1016/j.colsurfb.2025.114685.
    [7]
    LuoD, DengM, NiP, et al. Nanoparticle-enhanced injectable GelMA composite hydrogel for gastrointestinal wound healing[J]. ACS Biomater Sci Eng, 2026, 12(1): 588-599. DOI: 10.1021/acsbiomaterials.5c01231.
    [8]
    YueK,Trujillo-de SantiagoG,AlvarezMM,et al.Synthesis, properties, and biomedical applications of gelatin methacryloyl (GelMA) hydrogels[J].Biomaterials,2015,73:254-271.DOI: 10.1016/j.biomaterials.2015.08.045.
    [9]
    RenL, ZhouX, FengL, et al. ROS-scavenging and oxygen-generating MgMn-LDH integrated smart injectable hydrogel for microenvironment-reprogrammable spinal cord injury repair[J]. Mater Adv, 2026: 7 (7): 3586-3607. DOI: 10.1039/d6ma00108d.
    [10]
    ZhangX,NanK,ZhangY,et al.A novel injectable hydrogel prepared from phenylboronic acid modified gelatin and oxidized-dextran for bone tissue engineering[J].Int J Biol Macromol,2024,261(Pt 1):129666.DOI: 10.1016/j.ijbiomac.2024.129666.
    [11]
    ZhuY,XiuZ,JiangX,et al.Injectable hydrogels with ROS-triggered drug release enable the co-delivery of antibacterial agent and anti-inflammatory nanoparticle for periodontitis treatment[J].J Nanobiotechnology,2025,23(1):205.DOI: 10.1186/s12951-025-03275-4.
    [12]
    WangKY,JinXY,MaYH,et al.Injectable stress relaxation gelatin-based hydrogels with positive surface charge for adsorption of aggrecan and facile cartilage tissue regeneration[J].J Nanobiotechnology,2021,19(1):214.DOI: 10.1186/s12951-021-00950-0.
    [13]
    GengX, LiuK, WangJ, et al. Preparation of ultra-small copper nanoparticles-loaded self-healing hydrogels with antibacterial, inflammation-suppressing and angiogenesis-enhancing properties for promoting diabetic wound healing[J]. Int J Nanomed, 2023, 18: 3339-3358. DOI: 10.2147/IJN.S399933.
    [14]
    BorkowG, MelamedE. The journey of copper-impregnated dressings in wound healing: From a medical hypothesis to clinical practice[J]. Biomedicines, 2025, 13(3): 562. DOI: 10.3390/biomedicines13030562.
    [15]
    顾雅男, 徐翔昊, 王彦平, 等. 氧化铈纳米酶-甲基丙烯酸酐化明胶水凝胶在小鼠全层皮肤缺损感染创面修复中的作用[J]. 中华烧伤与创面修复杂志, 2024, 40(2): 131-140. DOI: 10.3760/cma.j.cn501225-20231120-00201.
    [16]
    GuoY, ZhangX, BanX, et al. Photothermally sterilizable hydrogel patch for accelerated burn wound healing[J]. Int J Biol Macromol, 2026,363:152156. DOI: 10.1016/j.ijbiomac.2026.152156.
    [17]
    HoEC, OlsonKE, ButlerM, et al. Clinical impact of pleural fluid Streptococcus pneumoniae polymerase chain reaction testing in children with complicated pneumonia[J]. Clin Infect Dis, 2024,79(6):1487-1494. DOI: 10.1093/cid/ciae439.
    [18]
    TangY, ZhaoR, YiM, et al. Multifunctional hydrogel enhances inflammatory control, antimicrobial activity, and oxygenation to promote healing in infectious wounds[J]. Biomacromolecules, 2024, 25(4): 2423-2437. DOI: 10.1021/acs.biomac.3c01386.
    [19]
    HanY, YinZ, WangY, et al. Photopolymerizable and antibacterial hydrogels loaded with metabolites from lacticaseibacillus rhamnosus GG for infected wound healing[J]. Biomacromolecules, 2024, 25(4): 2587-2596. DOI: 10.1021/acs.biomac.4c00124.
    [20]
    AbbasA, LiN, NaseerS, et al. Development of curcumin/ADP-loaded gelatin methacrylate hydrogel for enhanced wound healing with hemostatic, anti-inflammatory, and antibacterial properties[J]. Gels, 2026,12(6):456. DOI: 10.3390/gels12060456.
    [21]
    TangY,PengF,LiC,et al.Nicotinamide mononucleotide modified CeO2 hydrogels promote diabetic wound healing by managing exudate and reducing inflammation[J].iScience,2026,29(2):114247.DOI: 10.1016/j.isci.2025.114247.
    [22]
    FuZ, ChenL, MoL, et al. Cerium oxide nanoparticles for enhanced healing of diabetic foot ulcers[J]. Front Bioeng Biotechnol, 13: 1695821. DOI: 10.3389/fbioe.2025.1695821.
    [23]
    ZhangP,LiY,TangY,et al.Copper-based metal-organic framework as a controllable nitric oxide-releasing vehicle for enhanced diabetic wound healing[J].ACS Appl Mater Interfaces,2020,12(16):18319-18331.DOI: 10.1021/acsami.0c01792.
    [24]
    LiuJ,ZhuY,FanY,et al.The pH-dependent multiple nanozyme activities of copper-cerium dioxide and its application in regulating intracellular oxygen and hydrogen peroxide levels[J].J Colloid Interface Sci,2024,654(Pt B):1054-1062.DOI: 10.1016/j.jcis.2023.10.050.
    [25]
    JinM, PengL, HuangY, et al. On-demand switching of a pH/ROS-responsive Ce single-atom nanozyme spray for infected wounds[J]. Nano Lett, 2026, 26(17): 5934-5944. DOI: 10.1021/acs.nanolett.6c00541.
    [26]
    LiM, DingQ, HuS, et al. A triple-responsive nanozyme platform of AgAu-CeO2 heterojunction integrated with probiotics for precision antibacterial therapy in periodontitis[J]. Biomaterials, 2026,327:123731. DOI: 10.1016/j.biomaterials.2025.123731.
    [27]
    WangW, CuiY, WeiX, et al. CuCo2O4 nanoflowers with multiple enzyme activities for treating bacterium-infected wounds via cuproptosis-like death[J]. ACS Nano, 2024, 18(24): 15845-15863. DOI: 10.1021/acsnano.4c02825.
    [28]
    XiangC,PuC,ZhongX,et al.Functional hydrogels promote chronic infectious wound healing by re-rousing macrophage M1 and inducing bacterial copper-like death[J].Mater Today Bio,2025,31:101571.DOI: 10.1016/j.mtbio.2025.101571.
    [29]
    KrishnamoorthiR,HsiaoCY,ChouYP,et al.Green synthesis of a bacitracin@Ag-CeO2 nanocomposite@hydrogel for dual antibiofilm and anti-inflammatory therapy against MRSA wound infections[J].J Mater Chem B,2026,14(13):4125-4143.DOI: 10.1039/d5tb02286j.
    [30]
    HouQ, LiuK, LianC, et al. A gelatin-based composite hydrogel with a "one stone, two birds" strategy for photothermal antibacterial and vascularization of infected wounds[J]. Biomacromolecules, 2023, 24(7): 3397-3410. DOI: 10.1021/acs.biomac.3c00471.
    [31]
    TerriacL,HelesbeuxJJ,MaugarsY,et al.Boronate ester hydrogels for biomedical applications: challenges and opportunities[J]. Chem Mater,2024,36(14):6674-6695.DOI: 10.1021/acs.chemmater.4c00507.
    [32]
    LiQ,SongH,LiS,et al.Macrophage metabolism reprogramming EGCG-Cu coordination capsules delivered in polyzwitterionic hydrogel for burn wound healing and regeneration[J].Bioact Mater,2023,29:251-264.DOI: 10.1016/j.bioactmat.2023.07.011.
    [33]
    ZhaoM,KangM,WangJ,et al.Stem cell-derived nanovesicles embedded in dual-layered hydrogel for programmed ROS regulation and comprehensive tissue regeneration in burn wound healing[J].Adv Mater,2024,36(32):e2401369.DOI: 10.1002/adma.202401369.
    [34]
    Shirvani-FilAbadiM,ReiisiS,ShirianS.Enhancement of wound healing through chitosan/gelatin/polyvinyl alcohol-based nanocomposites containing chrysin-loaded cerium oxide nanoparticles: a synergistic approach to tissue regeneration[J].J Biomater Sci Polym Ed,2026:1-25.DOI: 10.1080/09205063.2026.2627417.
    [35]
    ZhaoS, LingJ, WangN, et al. Cerium dioxide nanozyme doped hybrid hydrogel with antioxidant and antibacterial abilities for promoting diabetic wound healing[J]. Chem Eng J, 2024, 497: 154517. DOI: 10.1016/j.cej.2024.154517.
    [36]
    SaravanakumarG,KimJ,KimWJ.Reactive-oxygen-species-responsive drug delivery systems: promises and challenges[J].Adv Sci (Weinh),2016,4(1):1600124.DOI: 10.1002/advs.201600124.
    [37]
    QiaoB,WangJ,QiaoL,et al.ROS-responsive hydrogels with spatiotemporally sequential delivery of antibacterial and anti-inflammatory drugs for the repair of MRSA-infected wounds[J].Regen Biomater,2024,11:rbad110.DOI: 10.1093/rb/rbad110.
    [38]
    TuC,LuH,ZhouT,et al.Promoting the healing of infected diabetic wound by an anti-bacterial and nano-enzyme-containing hydrogel with inflammation-suppressing, ROS-scavenging, oxygen and nitric oxide-generating properties[J].Biomaterials,2022,286:121597.DOI: 10.1016/j.biomaterials.2022.121597.
    • Relative Articles
    • Supplements(0)
    • Cited By
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(6)  / Tables(2)

    Get Citation
    PDF
    XML

    Article Metrics

    Article views (101) PDF downloads(12) Cited by()
    Proportional views
    Related

    Copyright © Chinese Journal of Burns京ICP备07035254号-14

    E-mail：shaoshangzazhi@163.com

    Website：https://zhsszz.xml-journal.net/

    Supported by: Beijing Renhe Information Technology Co. Ltd    

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return