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Volume 37 Issue 9
Sep.  2021
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Article Navigation >  CHINESE JOURNAL OF BURNS AND WOUNDS  > 2021  >  37(9): 860-868
Ye XY,Li XL,Li YG,et al.Clinical effects of medical ozone autologous blood transfusion combined with Xingnaojing in the treatment of septic encephalopathy in burns[J].Chin J Burns,2021,37(6):568-574.DOI: 10.3760/cma.j.cn501120-20200330-00204.
Citation: Zhang Y,Han F,He T,et al.Effects and mechanism of hepatocyte growth factor-modified human adipose mesenchymal stem cells on wound healing of full-thickness skin defects in diabetic rats[J].Chin J Burns,2021,37(9):860-868.DOI: 10.3760/cma.j.cn501120-20200626-00329.
shu

Effects and mechanism of hepatocyte growth factor-modified human adipose mesenchymal stem cells on wound healing of full-thickness skin defects in diabetic rats

doi: 10.3760/cma.j.cn501120-20200626-00329

  • Department of Burns and Cutaneous Surgery, Burn Center of PLA, the First Affiliated Hospital of Air Force Medical University, Xi'an 710032, China

Funds:

General Program of National Natural Science Foundation of China 81871561

Youth Science Foundation of National Natural Science Foundation of China 82002039

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    Abstract
  •   Objective  To investigate the effects and mechanism of hepatocyte growth factor (HGF)-modified human adipose mesenchymal stem cells (ADSCs) on the wound healing of full-thickness skin defects in diabetic rats.  Methods  The experimental research method was adopted. The discarded abdominal adipose tissue was collected from a 35-year-old healthy female who underwent abdominal liposuction in the Department of Plastic Surgery of the First Affiliated Hospital of Air Force Medical University in December 2019. The long spindle-shaped primary ADSCs were obtained by collagenase digestion, and the third passage of cells were identified by flow cytometry to positively express ADSCs surface markers CD29 and CD90 and negatively express CD34 and CD45. The third passage of ADSCs were used for the subsequent experiments. ADSCs were transfected with lentivirus-mediated HGF for 4 h (obtaining HGF modified ADSCs) and then routinely cultured for 24 h. The cell morphology was observed under an inverted phase contrast microscope, and the transfection rate was calculated. Eighty-one male Sprague-Dawley rats aged 4 weeks were induced into diabetic rat model by high glucose and high fat diet combined with streptozotocin injection. A full-thickness skin defect wound of 1.5 cm×1.5 cm was made on the back of each rat. The injured rats were divided into phosphate buffer solution (PBS) group, ADSCs alone group, and HGF-modified ADSCs group according to the random number table, with 27 rats in each group. The rats were injected with the same volume of corresponding substances around the wound on post injury day (PID) 1, 3, and 7, respectively. Nine rats in each group were selected according to the random number table, the wound area of whom was measured on PID 0 (immediately), 3, 7, 10, and 14 (after injection on injection day), and the wound healing rates on PID 3, 7, 10, and 14 were calculated. Nine remaining rats in each group were sacrificed after injection on PID 3 and 7, respectively, and the skin tissue around the wound were collected. The mRNA expressions of inflammatory factors such as tumor necrosis factor α (TNF-α), interleukin 1β (IL-1β), and IL-10 on PID 3 and collagen type Ⅰ and Ⅲ on PID 7 were detected by real-time fluorescent quantitative reverse transcription polymerase chain reaction. The expression level of vascular endothelial growth factor (VEGF) was detected by enzyme-linked immunosorbent assay on PID 7. The protein expression of nuclear factor κb-p65 on PID 3 and phosphorylation level of protein kinase B (Akt) on PID 7 were detected by Western blotting. Data were statistically analyzed with analysis of variance for repeated measurement, one-way analysis of variance, least significant difference t test, and Bonferroni correction.  Results  After 24 h of culture, the HGF-transfected human ADSCs showed good morphology, which was not different with the non-transfected ADSCs, and the transfection rate reached 90%. On PID 3, 7, 10, and 14, the wound healing rates of rats in HGF-modified ADSCs group were (31.5±1.0)%, (75.2±2.0)%, (92.2±1.3)%, and (99.1±1.8)%, respectively, being significantly higher than (21.4±1.3)%, (61.4±1.5)%, (80.1±2.1)%, and (92.4±1.8)% in PBS group and (25.1±2.1)%, (67.2±1.3)%, (89.3±1.4)%, and (95.1±2.1)% in ADSCs alone group (t=1.452, 0.393, 0.436, 0.211, 4.982, 3.011, 4.211, 7.503, P<0.05 or P<0.01). On PID 3, compared with those in PBS group and ADSCs alone group, the mRNA expressions of TNF-α and IL-1β and protein expression of nuclear factor κb-p65 in the skin tissue around the wound of rats in HGF-modified ADSCs group were significantly decreased (t=7.281, 17.700, 9.447, 6.231, 13.083, 7.783, P<0.01), and the mRNA expression of IL-10 in the skin tissue around the wound of rats in HGF-modified ADSCs group was significantly increased (t=-6.644, -6.381, P<0.01). On PID 7, compared with those in PBS group and ADSCs alone group, the mRNA expressions of collagen type Ⅰ and Ⅲ, the expression level of VEGF, and the phosphorylation level of Akt in the skin tissue around the wound of rats in HGF-modified ADSCs group were significantly increased (t=-5.126, -4.347, -5.058, -3.367, -10.694, -19.876, -4.890, -6.819, P<0.05 or P<0.01).  Conclusions  HGF-modified human ADSCs can significantly promote the wound healing of full-thickness skin defects in diabetic rats. The mechanism may be related to the inhibition of TNF-α and IL-1β expression, the promotion of IL-10, collagen type Ⅰ and Ⅲ, and VEGF expression, which could be related to the inhibition of nuclear factor κB signaling pathway, and the promotion of Akt signaling pathway.

     

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  • [1]
    MirzaR,KohTJ.Dysregulation of monocyte/macrophage phenotype in wounds of diabetic mice[J].Cytokine,2011,56(2):256-264.DOI: 10.1016/j.cyto.2011.06.016.
    [2]
    姜玉峰.体表慢性难愈合创面的研究进展[J].感染、炎症、修复,2011,12(1):59-61.DOI: 10.3969/j.issn.1672-8521.2011.01.024.
    [3]
    CaoHM,ChengYQ,GaoHQ,et al.In vivo tracking of mesenchymal stem cell-derived extracellular vesicles improving mitochondrial function in renal ischemia-reperfusion injury[J].ACS Nano,2020,14(4):4014-4026.DOI: 10.1021/acsnano.9b08207.
    [4]
    YanWJ,LinC,GuoYZ,et al.N-cadherin overexpression mobilizes the protective effects of mesenchymal stromal cells against ischemic heart injury through a β-catenin-dependent manner[J].Circ Res,2020,126(7):857-874.DOI: 10.1161/CIRCRESAHA.119.315806.
    [5]
    BaoHY,XiaYY,YuCG,et al.CT/bioluminescence dual-modal imaging tracking of mesenchymal stem cells in pulmonary fibrosis[J].Small,2019,15(46):e1904314.DOI: 10.1002/smll.201904314.
    [6]
    LiX,HuYD,GuoY,et al.Safety and efficacy of intracoronary human umbilical cord-derived mesenchymal stem cell treatment for very old patients with coronary chronic total occlusion[J].Curr Pharm Des,2015,21(11):1426-1432.DOI: 10.2174/1381612821666141126100636.
    [7]
    SunB,MengXH,LiuR,et al.Mechanism study for hypoxia induced differentiation of insulin-producing cells from umbilical cord blood-derived mesenchymal stem cells[J].Biochem Biophys Res Commun,2015,466(3):444-449.DOI: 10.1016/j.bbrc.2015.09.047.
    [8]
    BroekmanW,AmatngalimGD,de Mooij-EijkY,et al.TNF-α and IL-1β-activated human mesenchymal stromal cells increase airway epithelial wound healing in vitro via activation of the epidermal growth factor receptor[J].Respir Res,2016,17:3.DOI: 10.1186/s12931-015-0316-1.
    [9]
    ValenteS,CiavarellaC,PasanisiE,et al.Hepatocyte growth factor effects on mesenchymal stem cells derived from human arteries: a novel strategy to accelerate vascular ulcer wound healing[J].Stem Cells Int,2016,2016:3232859.DOI: 10.1155/2016/3232859.
    [10]
    李雪阳,郑万玲,杨超,等.HGF/c-Met反应轴对脂肪干细胞修复烧伤创面的调控[J].中国组织工程研究,2018,22(25):3975-3980.DOI: 10.3969/j.issn.2095-4344.0929.
    [11]
    FuseMA,PlatiSK,BurnsSS,et al.Combination therapy with c-Met and Src inhibitors induces caspase-dependent apoptosis of merlin-deficient Schwann cells and suppresses growth of schwannoma cells[J].Mol Cancer Ther,2017,16(11):2387-2398.DOI: 10.1158/1535-7163.MCT-17-0417.
    [12]
    LiXQ,WuGF,HanF,et al.SIRT1 activation promotes angiogenesis in diabetic wounds by protecting endothelial cells against oxidative stress[J].Arch Biochem Biophys,2019,661:117-124.DOI: 10.1016/j.abb.2018.11.016.
    [13]
    GongJH,DongJY,XieT,et al.The influence of AGEs environment on proliferation, apoptosis, homeostasis, and endothelial cell differentiation of human adipose stem cells[J].Int J Low Extrem Wounds,2017,16(2):94-103.DOI: 10.1177/1534734617701575.
    [14]
    ZhangW,BaiXZ,ZhaoB,et al.Cell-free therapy based on adipose tissue stem cell-derived exosomes promotes wound healing via the PI3K/Akt signaling pathway[J].Exp Cell Res,2018,370(2):333-342.DOI: 10.1016/j.yexcr.2018.06.035.
    [15]
    秦逸人,刘慧雯,王锦绣,等.干细胞治疗糖尿病的研究现状及未来[J].中国组织工程研究与临床康复,2007,11(24):4802-4805.DOI: 10.3321/j.issn:1673-8225.2007.24.045.
    [16]
    KolarMK,KinghamPJ.Regenerative effects of adipose-tissue-derived stem cells for treatment of peripheral nerve injuries[J].Biochem Soc Trans,2014,42(3):697-701.DOI: 10.1042/BST20140004.
    [17]
    PhinneyDG,PittengerMF.Concise review: MSC-derived exosomes for cell-free therapy[J].Stem Cells,2017,35(4):851-858.DOI: 10.1002/stem.2575.
    [18]
    LiuXY,WangZ,WangR,et al.Direct comparison of the potency of human mesenchymal stem cells derived from amnion tissue, bone marrow and adipose tissue at inducing dermal fibroblast responses to cutaneous wounds[J].Int J Mol Med,2013,31(2):407-415.DOI: 10.3892/ijmm.2012.1199.
    [19]
    王哲,张殿宝,刘晓玉,等.正常与糖尿病小鼠脂肪间充质干细胞移植促进皮肤创伤愈合的比较[J].解剖科学进展,2014,20(5):420-424.
    [20]
    FiorinaP,PietramaggioriG,SchererSS,et al.The mobilization and effect of endogenous bone marrow progenitor cells in diabetic wound healing[J].Cell Transplant,2010,19(11):1369-1381.DOI: 10.3727/096368910X514288.
    [21]
    张广德,李荣亮,岳从雷,等.腺病毒介导HGF转染脂肪干细胞复合温敏型可注射水凝胶对兔颞下颌关节骨关节病髁突软骨的修复作用[J].口腔医学研究,2017,33(9):924-927.DOI: 10.13701/j.cnki.kqyxyj.2017.09.004.
    [22]
    LeeJS,RobertsonA,CooperMA,et al.The small molecule NLRP3 inflammasome inhibitor MCC950 does not alter wound healing in obese mice[J].Int J Mol Sci,2018,19(11):3289.DOI: 10.3390/ijms19113289.
    [23]
    SharmaD,KannegantiTD.The cell biology of inflammasomes: mechanisms of inflammasome activation and regulation[J].J Cell Biol,2016,213(6):617-629.DOI: 10.1083/jcb.201602089.
    [24]
    RomeroN,Van WaesbergheC,FavoreelHW.Pseudorabies virus infection of epithelial cells leads to persistent but aberrant activation of the NF-κB pathway, inhibiting hallmark NF-κB-induced proinflammatory gene expression[J].J Virol,2020,94(10):e00196-20.DOI: 10.1128/JVI.00196-20.
    [25]
    MothesJ,BusseD,KofahlB,et al.Sources of dynamic variability in NF-κB signal transduction: a mechanistic model[J].Bioessays,2015,37(4):452-462.DOI: 10.1002/bies.201400113.
    [26]
    MiraghazadehB,CookMC.Nuclear factor-kappaB in autoimmunity: man and mouse[J].Front Immunol,2018,9:613.DOI: 10.3389/fimmu.2018.00613.
    [27]
    ZhangQ,LenardoMJ,BaltimoreD.30 years of NF-κB: a blossoming of relevance to human pathobiology[J].Cell,2017,168(1/2):37-57.DOI: 10.1016/j.cell.2016.12.012.
    [28]
    NaJ,ShinJY,JeongH,et al.JMJD3 and NF-κB-dependent activation of Notch1 gene is required for keratinocyte migration during skin wound healing[J].Sci Rep,2017,7(1):6494.DOI: 10.1038/s41598-017-06750-7.
    [29]
    HaydenMS,GhoshS.Regulation of NF-κB by TNF family cytokines[J].Semin Immunol,2014,26(3):253-266.DOI: 10.1016/j.smim.2014.05.004.
    [30]
    VallabhapurapuS,KarinM.Regulation and function of NF-kappaB transcription factors in the immune system[J].Annu Rev Immunol,2009,27:693-733.DOI: 10.1146/annurev.immunol.021908.132641.
    [31]
    SeflekHN,KalkanS,CuceG,et al.Effects of Nigella sativa oil on ovarian volume, oxidant systems, XIAP and NF-kB expression in an experimental model of diabetes[J].Biotech Histochem,2019,94(5):325-333.DOI: 10.1080/10520295.2019.1566571.
    [32]
    TonioloA,CassaniG,PuggioniA,et al.The diabetes pandemic and associated infections: suggestions for clinical microbiology[J].Rev Med Microbiol,2019,30(1):1-17.DOI: 10.1097/MRM.0000000000000155.
    [33]
    MartinP.Wound healing--aiming for perfect skin regeneration[J].Science,1997,276(5309):75-81.DOI: 10.1126/science.276.5309.75.
    [34]
    WernerS,KriegT,SmolaH.Keratinocyte-fibroblast interactions in wound healing[J].J Invest Dermatol,2007,127(5):998-1008.DOI: 10.1038/sj.jid.5700786.
    [35]
    ShiHX,XieHH,ZhaoY,et al.Myoprotective effects of bFGF on skeletal muscle injury in pressure-related deep tissue injury in rats[J].Burns Trauma,2016,4:26.DOI: 10.1186/s41038-016-0051-y.
    [36]
    SenT,SahaP,JiangT,et al.Sulfhydration of AKT triggers Tau-phosphorylation by activating glycogen synthase kinase 3β in Alzheimer's disease[J].Proc Natl Acad Sci U S A,2020,117(8):4418-4427.DOI: 10.1073/pnas.1916895117.
    [37]
    HinzN,JückerM.Distinct functions of AKT isoforms in breast cancer: a comprehensive review[J].Cell Commun Signal,2019,17(1):154.DOI: 10.1186/s12964-019-0450-3.
    [38]
    MaroulakouIG,OemlerW,NaberSP,et al.Distinct roles of the three Akt isoforms in lactogenic differentiation and involution[J].J Cell Physiol,2008,217(2):468-477.DOI: 10.1002/jcp.21518.
    [39]
    YangHL,TsaiYC,KoriviM,et al.Lucidone promotes the cutaneous wound healing process via activation of the PI3K/AKT, Wnt/β-catenin and NF-κB signaling pathways[J].Biochim Biophys Acta Mol Cell Res,2017,1864(1):151-168.DOI: 10.1016/j.bbamcr.2016.10.021.
    [40]
    SugiyamaMG,FairnGD,AntonescuCN.Akt-ing up just about everywhere: compartment-specific Akt activation and function in receptor tyrosine kinase signaling[J].Front Cell Dev Biol,2019,7:70.DOI: 10.3389/fcell.2019.00070.
    [41]
    Ciruelos GilEM.Targeting the PI3K/AKT/mTOR pathway in estrogen receptor-positive breast cancer[J].Cancer Treat Rev,2014,40(7):862-871.DOI: 10.1016/j.ctrv.2014.03.004.
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    Created with Highcharts 5.0.7Chart context menuAccess Area Distribution其他: 1.0 %其他: 1.0 %其他: 0.5 %其他: 0.5 %China: 0.3 %China: 0.3 %Seattle: 0.0 %Seattle: 0.0 %United States: 0.0 %United States: 0.0 %[]: 0.3 %[]: 0.3 %三明: 0.4 %三明: 0.4 %三门峡: 0.5 %三门峡: 0.5 %上海: 0.1 %上海: 0.1 %东莞: 0.2 %东莞: 0.2 %丽水: 1.0 %丽水: 1.0 %乐山: 0.3 %乐山: 0.3 %佛山: 0.1 %佛山: 0.1 %保定: 0.6 %保定: 0.6 %信阳: 0.0 %信阳: 0.0 %克拉玛依: 0.0 %克拉玛依: 0.0 %六安: 0.4 %六安: 0.4 %六盘水: 0.2 %六盘水: 0.2 %兴安: 0.1 %兴安: 0.1 %兴安盟: 0.0 %兴安盟: 0.0 %凉山彝族自治州: 0.0 %凉山彝族自治州: 0.0 %包头: 0.2 %包头: 0.2 %北京: 0.2 %北京: 0.2 %北方邦: 0.0 %北方邦: 0.0 %南京: 0.3 %南京: 0.3 %南充: 0.1 %南充: 0.1 %南平: 1.6 %南平: 1.6 %南昌: 0.1 %南昌: 0.1 %南通: 0.9 %南通: 0.9 %厦门: 0.2 %厦门: 0.2 %台北: 0.1 %台北: 0.1 %台州: 0.5 %台州: 0.5 %合肥: 0.1 %合肥: 0.1 %吉林: 0.4 %吉林: 0.4 %呼和浩特: 0.1 %呼和浩特: 0.1 %咸阳: 0.1 %咸阳: 0.1 %哈尔滨: 0.3 %哈尔滨: 0.3 %哥伦布: 0.1 %哥伦布: 0.1 %唐山: 0.6 %唐山: 0.6 %商丘: 0.0 %商丘: 0.0 %喀什: 0.0 %喀什: 0.0 %嘉兴: 1.7 %嘉兴: 1.7 %大连: 1.9 %大连: 1.9 %天津: 0.3 %天津: 0.3 %宁德: 3.2 %宁德: 3.2 %宁波: 0.9 %宁波: 0.9 %安庆: 0.1 %安庆: 0.1 %安康: 1.6 %安康: 1.6 %宜春: 0.3 %宜春: 0.3 %宣城: 0.0 %宣城: 0.0 %宿迁: 3.4 %宿迁: 3.4 %山景城: 0.1 %山景城: 0.1 %常德: 0.9 %常德: 0.9 %广元: 0.3 %广元: 0.3 %广安: 0.3 %广安: 0.3 %广州: 1.6 %广州: 1.6 %廊坊: 0.1 %廊坊: 0.1 %延安: 4.4 %延安: 4.4 %延边朝鲜族自治州: 0.1 %延边朝鲜族自治州: 0.1 %张家口: 0.7 %张家口: 0.7 %徐州: 1.5 %徐州: 1.5 %德州: 0.1 %德州: 0.1 %德阳: 0.7 %德阳: 0.7 %怀化: 0.1 %怀化: 0.1 %成都: 0.1 %成都: 0.1 %扬州: 0.5 %扬州: 0.5 %抚州: 1.0 %抚州: 1.0 %抚顺: 0.3 %抚顺: 0.3 %拉贾斯坦邦: 0.1 %拉贾斯坦邦: 0.1 %无锡: 0.6 %无锡: 0.6 %日照: 1.2 %日照: 1.2 %昆明: 0.0 %昆明: 0.0 %普赖恩维尔: 0.1 %普赖恩维尔: 0.1 %朝阳: 0.2 %朝阳: 0.2 %杭州: 1.7 %杭州: 1.7 %枣庄: 0.0 %枣庄: 0.0 %株洲: 0.1 %株洲: 0.1 %榆林: 0.7 %榆林: 0.7 %武汉: 0.2 %武汉: 0.2 %汉中: 0.8 %汉中: 0.8 %汕头: 0.6 %汕头: 0.6 %池州: 0.9 %池州: 0.9 %沃思堡: 0.2 %沃思堡: 0.2 %沈阳: 1.2 %沈阳: 1.2 %泉州: 1.1 %泉州: 1.1 %泰州: 1.4 %泰州: 1.4 %泸州: 0.5 %泸州: 0.5 %济南: 1.3 %济南: 1.3 %海得拉巴: 0.1 %海得拉巴: 0.1 %淄博: 0.1 %淄博: 0.1 %淮北: 0.2 %淮北: 0.2 %淮安: 0.5 %淮安: 0.5 %温州: 1.2 %温州: 1.2 %渭南: 0.5 %渭南: 0.5 %湖州: 1.8 %湖州: 1.8 %湘潭: 0.4 %湘潭: 0.4 %湘西: 0.6 %湘西: 0.6 %湘西土家族苗族自治州: 0.3 %湘西土家族苗族自治州: 0.3 %滁州: 0.3 %滁州: 0.3 %漯河: 0.1 %漯河: 0.1 %漳州: 1.2 %漳州: 1.2 %烟台: 0.7 %烟台: 0.7 %白山: 0.3 %白山: 0.3 %盐城: 0.4 %盐城: 0.4 %盘锦: 0.9 %盘锦: 0.9 %石嘴山: 0.1 %石嘴山: 0.1 %石家庄: 0.8 %石家庄: 0.8 %福州: 0.2 %福州: 0.2 %科尼亚: 0.3 %科尼亚: 0.3 %秦皇岛: 0.2 %秦皇岛: 0.2 %绍兴: 0.5 %绍兴: 0.5 %绵阳: 0.4 %绵阳: 0.4 %罗奥尔凯埃: 0.1 %罗奥尔凯埃: 0.1 %自贡: 0.3 %自贡: 0.3 %舟山: 0.4 %舟山: 0.4 %芒廷维尤: 6.8 %芒廷维尤: 6.8 %芜湖: 0.5 %芜湖: 0.5 %芝加哥: 0.0 %芝加哥: 0.0 %苏州: 0.3 %苏州: 0.3 %荆门: 1.4 %荆门: 1.4 %莆田: 2.5 %莆田: 2.5 %营口: 1.4 %营口: 1.4 %葫芦岛: 0.2 %葫芦岛: 0.2 %蚌埠: 0.0 %蚌埠: 0.0 %衡水: 0.4 %衡水: 0.4 %衢州: 1.8 %衢州: 1.8 %襄阳: 0.4 %襄阳: 0.4 %西宁: 0.3 %西宁: 0.3 %西安: 2.5 %西安: 2.5 %西雅图: 0.0 %西雅图: 0.0 %许昌: 0.1 %许昌: 0.1 %辽阳: 1.1 %辽阳: 1.1 %达州: 0.2 %达州: 0.2 %运城: 0.1 %运城: 0.1 %连云港: 0.9 %连云港: 0.9 %通辽: 0.1 %通辽: 0.1 %遵义: 0.0 %遵义: 0.0 %邢台: 0.0 %邢台: 0.0 %邵阳: 0.2 %邵阳: 0.2 %郑州: 0.0 %郑州: 0.0 %郴州: 1.5 %郴州: 1.5 %鄂尔多斯: 0.5 %鄂尔多斯: 0.5 %重庆: 3.4 %重庆: 3.4 %金华: 1.1 %金华: 1.1 %铁岭: 0.9 %铁岭: 0.9 %铜川: 0.1 %铜川: 0.1 %铜陵: 1.6 %铜陵: 1.6 %银川: 0.1 %银川: 0.1 %锦州: 1.3 %锦州: 1.3 %长春: 0.1 %长春: 0.1 %长沙: 0.5 %长沙: 0.5 %雅安: 0.2 %雅安: 0.2 %鞍山: 2.2 %鞍山: 2.2 %韶关: 0.0 %韶关: 0.0 %马鞍山: 0.1 %马鞍山: 0.1 %驻马店: 0.0 %驻马店: 0.0 %鹰潭: 0.2 %鹰潭: 0.2 %黄山: 0.3 %黄山: 0.3 %黄石: 0.5 %黄石: 0.5 %黔西南: 0.4 %黔西南: 0.4 %齐齐哈尔: 0.0 %齐齐哈尔: 0.0 %其他其他ChinaSeattleUnited States[]三明三门峡上海东莞丽水乐山佛山保定信阳克拉玛依六安六盘水兴安兴安盟凉山彝族自治州包头北京北方邦南京南充南平南昌南通厦门台北台州合肥吉林呼和浩特咸阳哈尔滨哥伦布唐山商丘喀什嘉兴大连天津宁德宁波安庆安康宜春宣城宿迁山景城常德广元广安广州廊坊延安延边朝鲜族自治州张家口徐州德州德阳怀化成都扬州抚州抚顺拉贾斯坦邦无锡日照昆明普赖恩维尔朝阳杭州枣庄株洲榆林武汉汉中汕头池州沃思堡沈阳泉州泰州泸州济南海得拉巴淄博淮北淮安温州渭南湖州湘潭湘西湘西土家族苗族自治州滁州漯河漳州烟台白山盐城盘锦石嘴山石家庄福州科尼亚秦皇岛绍兴绵阳罗奥尔凯埃自贡舟山芒廷维尤芜湖芝加哥苏州荆门莆田营口葫芦岛蚌埠衡水衢州襄阳西宁西安西雅图许昌辽阳达州运城连云港通辽遵义邢台邵阳郑州郴州鄂尔多斯重庆金华铁岭铜川铜陵银川锦州长春长沙雅安鞍山韶关马鞍山驻马店鹰潭黄山黄石黔西南齐齐哈尔

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      沈阳化工大学材料科学与工程学院 沈阳 110142

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