Email Alerts
RSS
Volume 37 Issue 8
Aug.  2021
Turn off MathJax
Article Contents
Article Navigation >  CHINESE JOURNAL OF BURNS AND WOUNDS  > 2021  >  37(8): 793-796
Zhou KQ,Su YJ,Jia CY.Reliability study on establishing a rat tuberculosis wound model using Bacillus Calmette-Guérin[J].Chin J Burns,2021,37(8):793-796.DOI: 10.3760/cma.j.cn501120-20200531-00290.
Citation: Zhou KQ,Su YJ,Jia CY.Reliability study on establishing a rat tuberculosis wound model using Bacillus Calmette-Guérin[J].Chin J Burns,2021,37(8):793-796.DOI: 10.3760/cma.j.cn501120-20200531-00290.
shu

Reliability study on establishing a rat tuberculosis wound model using Bacillus Calmette-Guérin

doi: 10.3760/cma.j.cn501120-20200531-00290
  • 1.

    Department of Plastic Surgery,Second Clinical Medical College of Shanxi Medical University,Taiyuan 030001, China

  • 2.

    Plastic Surgery Hospital, Xi'an International Medical Center, Xi'an 710118, China

  • 3.

    Department of Burns and Plastic SurgeryWound Repair Surgery, Xiang'an Hospital of Xiamen University, Xiamen 361102, China

Funds:

Natural Science Foundation of Fujian Province of China 2019J01011

Starting Package of Xiang'an Hospital of Xiamen University PM201809170010

More Information
  • Corresponding author: Jia Chiyu, Email: jiachiyu@qq.com
  • Received Date: 2020-05-31
    Abstract
  •   Objective  To evaluate the reliability of a rat tuberculous wound model established by injecting Bacillus Calmette-Guérin (BCG).  Methods  The experimental research was conducted. According to the random number table, fifteen 6-week-old male Sprague-Dawley rats were divided into normal control group and infection group, with 3 rats in normal control group and 12 rats in infection group. Rats in infection group were injected with Freund's complete adjuvant, 3 weeks later, they were injected subcutaneously with BCG bacterial solution to establish a model of tuberculous wounds in rats; rats in normal control group did not receive any treatment. On the 8th, 15th, 32nd, and 43rd day of infection, the skin condition at the injection sites of the rats in infection group was observed roughly. Skin tissue at the injection sites of 3 rats in infection group at each corresponding time point stated above and skin tissue at the corresponding sites of the rats in normal control group were stained with hematoxylin-eosin to observe the cell arrangement, necrosis and inflammation. On 43rd day of infection, acid-fast staining was performed on the skin tissue at the injection sites of the rats in infection group to observe the distribution of bacteria.  Results  On the 8th, 15th, 32nd, and 43rd day of infection, tuberculous wound lesions were gradually developed at the skin tissues at the injection sites of the rats in infection group. The cells of the diseased tissue of the rats in infection group arranged disorderly or concentrically, and the number of granulomas and necrotic cells gradually increased, while the skin tissue cells in the corresponding parts of the rats in normal control group arranged regularly with no inflammatory cell infiltration. On the 43rd day of infection, a large number of rod-shaped bacteria were observed in the skin tissue at the injection sites of the rats in infection group.  Conclusions  The rat tuberculous wound model established using BCG is stable and reliable, which can meet the experimental requirements.

     

    • FullText(HTML)
  • loading
    • References(23)
  • [1]
    World Health OrganizationGlobal tuberculosis report2020-05-31https://www.who.int/teams/global-tuberculosis-programme/data

    World Health Organization.Global tuberculosis report[R/OL].2018[2020-05-31]. https://www.who.int/teams/global-tuberculosis-programme/data.
    [2]
    World Health OrganizationGlobal tuberculosis report2020-05-31https://www.who.int/teams/global-tuberculosis-programme/data

    World Health Organization.Global tuberculosis report[R/OL].2017[2020-05-31]. https://www.who.int/teams/global-tuberculosis-programme/data.
    [3]
    贾赤宇.结核性创面——一个被忽视且值得重视的临床问题[J/CD].中华损伤与修复杂志:电子版,2014,9(4):9-11.DOI: 10.3877/cma.j.issn.1673-9450.2014.04.003.
    [4]
    SahinN,AydinNE,SenolM,et al.Longstanding skin ulcers due to Mycobacterium tuberculosis in a healthy man[J].Trop Biomed,2010,27(1):120-124.
    [5]
    荣美玉,贾赤宇.单中心结核性创面与非结核性慢性难愈性创面患者的特点[J].中华烧伤杂志,2019,35(2):90-94.DOI: 10.3760/cma.j.issn.1009-2587.2019.02.003.
    [6]
    常娜,贾赤宇,刘真,等.235例肺外结核性创面患者流行病学调查[J].中华烧伤杂志,2015,31(2):122-124.DOI: 10.3760/cma.j.issn.1009-2587.2015.02.011.
    [7]
    贾赤宇,李鹏程,程琳,等.外科干预治疗模式在窦道型结核性创面中的临床应用[J].中华烧伤杂志,2016,32(6):326-330.DOI: 10.3760/cma.j.issn.1009-2587.2016.06.003.
    [8]
    吕晓武,贾赤宇,冯胜娟,等.胸壁结核性创面外科治疗进展[J].感染、炎症、修复,2014,15(2):122-124.DOI: 10.3969/j.issn.1672-8521.2014.02.021.
    [9]
    秦云贺,王艺红,郭庆龙,等.结核分枝杆菌菌悬液光密度值与菌落计数的关联性[J].中国感染控制杂志,2016,15(3):150-154.DOI: 10.3969/j.issn.1671-9638.2016.03.002.
    [10]
    DannenbergAM.Liquefaction and cavity formation in pulmonary TB: a simple method in rabbit skin to test inhibitors[J].Tuberculosis (Edinb),2009,89(4):243-247.DOI: 10.1016/j.tube.2009.05.006.
    [11]
    HusainAA,DaginawalaHF,WarkeSR,et al.Investigation of immune biomarkers using subcutaneous model of M. tuberculosis infection in BALB/c mice: a preliminary report[J].Immune Netw,2015,15(2):83-90.DOI: 10.4110/in.2015.15.2.83.
    [12]
    张同威,汪毅平,贾赤宇.清创后外用碱性成纤维细胞生长因子对新西兰兔结核性创面愈合的影响[J].中华烧伤杂志,2019,35(2):95-103.DOI: 10.3760/cma.j.issn.1009-2587.2019.02.004.
    [13]
    汪毅平,刘真,张亚洁,等.结核性创面大鼠模型中巨噬细胞极化改变[J/CD].中华损伤与修复杂志:电子版,2018,13(1):30-36.DOI: 10.3877/cma.j.issn.1673-9450.2018.01.007.
    [14]
    ChenL,LiuZ,SuY,et al.Characterization of Mycobacterium marinum infections in zebrafish wounds and sinus tracts[J].Wound Repair Regen,2017,25(3):536-540.DOI: 10.1111/wrr.12540.
    [15]
    ImamuraT,IyamaK,TakeyaM,et al.Role of macrophage tissue factor in the development of the delayed hypersensitivity reaction in monkey skin[J].Cell Immunol,1993,152(2):614-622.DOI: 10.1006/cimm.1993.1317.
    [16]
    汪毅平,张同威,贾赤宇.结核性创面动物模型的研究进展[J/CD].中华损伤与修复杂志:电子版,2017,12(4):309-311.DOI: 10.3877/cma.j.issn.1673-9450.2017.04.014.
    [17]
    ZhangG,ZhuB,ShiW,et al.Evaluation of mycobacterial virulence using rabbit skin liquefaction model[J].Virulence,2010,1(3):156-163.DOI: 10.4161/viru.1.3.11748.
    [18]
    DongX,LuoY,GaoQ,et al.Effects of MBL-associated serine protease-2 (MASP-2) on liquefaction and ulceration in rabbit skin model of tuberculosis[J].Microb Pathog,2016,99:282-286.DOI: 10.1016/j.micpath.2016.08.037.
    [19]
    王明珠,史大中,杨爱军,等.分枝杆菌所致家兔皮肤液化病理模型研究[J].微生物与感染,2008,3(4):208-211,218.DOI: 10.3969/j.issn.1673-6184.2008.04.004.
    [20]
    SugaM,DannenbergAM,HiguchiS.Macrophage functional heterogeneity in vivo. Macrolocal and microlocal macrophage activation, identified by double-staining tissue sections of BCG granulomas for pairs of enzymes[J].Am J Pathol,1980,99(2):305-323.
    [21]
    HiguchiS,SugaM,DannenbergAM,et al.Persistence of protein, carbohydrate and was components of tubercle bacilli in dermal BCG lesions[J].Am Rev Respir Dis,1981,123(4 Pt 1):397-401.DOI: 10.1164/arrd.1981.123.4.397.
    [22]
    ChenH,LiuX,MaX,et al.A new rabbit-skin model to evaluate protective efficacy of tuberculosis vaccines[J].Front Microbiol,2017,8:842.DOI: 10.3389/fmicb.2017.00842.
    [23]
    GutierrezMG,MasterSS,SinghSB,et al.Autophagy is a defense mechanism inhibiting BCG and Mycobacterium tuberculosis survival in infected macrophages[J].Cell,2004,119(6):753-766.DOI: 10.1016/j.cell.2004.11.038.
    • Relative Articles
    • Supplements(0)
    • Cited By
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(3)

    Get Citation
    PDF
    XML

    Article Metrics

    Article views (181) PDF downloads(7) Cited by()
    Proportional views
    Related

    Copyright © Chinese Journal of Burns京ICP备07035254号-14

    E-mail：shaoshangzazhi@163.com

    Website：https://zhsszz.xml-journal.net/

    Supported by: Beijing Renhe Information Technology Co. Ltd    

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return