Zhu DZ,Yao B,Cui XL,et al.Effects and mechanism of age on the stiffness and the fibrotic phenotype of fibroblasts of human hypertrophic scar[J].Chin J Burns,2021,37(10):937-945.DOI: 10.3760/cma.j.cn501120-20200810-00374.
Citation: Zhu DZ,Yao B,Cui XL,et al.Effects and mechanism of age on the stiffness and the fibrotic phenotype of fibroblasts of human hypertrophic scar[J].Chin J Burns,2021,37(10):937-945.DOI: 10.3760/cma.j.cn501120-20200810-00374.

Effects and mechanism of age on the stiffness and the fibrotic phenotype of fibroblasts of human hypertrophic scar

doi: 10.3760/cma.j.cn501120-20200810-00374
Funds:

General Program of National Natural Science Foundation of China 32000969, 82002056

Military Medicine Innovation Research program of Chinese PLA General Program CX19026

Wang Zhengguo Foundation for Traumatic Medicine Growth Factor Rejuvenation Plan SZYZ-TR-03

More Information
  • Corresponding author: Huang Sha, Email: stellarahuang@sina.com
  • Received Date: 2020-08-10
  •   Objective  To explore the effects and potential molecular mechanism of age on the stiffness and the fibrotic phenotype of fibroblasts (Fbs) of human hypertrophic scar.  Methods  The experimental research method was used. From January to June 2020, the surgically removed hypertrophic scar tissue of 10 scar patients (4 males and 6 females) and residual full-thickness normal skin tissue of 10 cases (5 males and 5 females, aged 7-41 years) were collected after operation in Department of Burns and Plastic Surgery of the Fourth Medical Center of the PLA General Hospital. The hypertrophic scar tissue of 6 patients aged (10.7±1.6) years was included into the young group and the hypertrophic scar tissue of 4 patients aged (40.0±2.2) years was included into the elderly group according to the age of patients. For the normal skin tissue and scar tissue in the two groups, hematoxylin eosin (HE) staining was performed to observe the tissue morphology, Masson staining was performed to observe the morphology and arrangement of collagen and quantify the content of collagen, and scanning electron microscope was used to observe the microscopic difference of dermal collagen fibers after the samples were freeze-dried and metal coated. The stiffness of scar tissue in the two groups was measured by atomic force microscope under the liquid phase. The scar tissue in the two groups was collected and the Fbs were isolated and cultured. The morphological differences of the Fbs were observed under the inverted phase contrast microscope, and the protein expression of paxillin was detected with cellular immunofluorescence to reflect the morphology of the Fbs. Cellular immunofluorescence was used to detect the expressions of pro-fibrosis protein α-smooth actin (α-SMA), transforming growth factor-β1 (TGF-β1), and type Ⅰ collagen, mechanotransduction-related protein Yes-associated protein (YAP), and the proliferation-related protein Ki67. Real-time fluorescent quantitative reverse transcription polymerase chain reaction was used to detect the mRNA expressions of pro-fibrosis genes of TGF-β1, α-SMA, and type Ⅰ collagen, fibrosis inhibiting gene of TGF-β3, and mechanotransduction-related genes of Rho-associated protein 1 (ROCK1) and YAP. Data were statistically analyzed with one-way analysis of variance and least significant difference t test.  Results  HE staining showed that the epidermal layer of normal skin was uneven, and blood vessels and sweat glands could be seen in the dermal layer; the epidermal layer of the scar tissue in the two groups was relatively flat, and blood vessels and sweat glands were rare. Masson staining and scanning electron microscopy showed that the collagen fibers in normal skin arranged loosely and disorderly, while the collagen fibers in scar tissue of the two groups arranged densely and orderly, and the collagen fibers in scar tissue of the young group were denser than those of the elderly group. The collagen content in scar tissue of the young group and the elderly group was significantly higher than that of the normal skin tissue (t=8.02, 3.15, P<0.05 or P<0.01), and the collagen content in scar tissue of the elderly group was significantly lower than that of the young group (t=4.84, P<0.05). The dermal stiffness of scar tissue in the elderly group was (50.3±1.1) kPa, significantly higher than (35.2±0.8) kPa in the young group (t=11.43, P<0.05). There were no obvious differences in the morphology of scar Fbs in the two groups observed under inverted phase contrast microscope and by cellular immunofluorescence. The expressions of type Ⅰ collagen and TGF-β1 in scar Fbs cytoplasm of the elderly group were significantly higher than those in the young group, while the expressions of α-SMA in scar Fbs cytoplasm were close in the two groups. The expressions of YAP in cytoplasm and nucleus of scar Fbs in the elderly group were significantly higher than those in the young group, while the expressions of Ki67 in scar Fbs nucleus of the two groups were close. The mRNA expressions of TGF-β1 and type Ⅰ collagen in scar Fbs of the elderly group were significantly higher than those in the young group (t=2.87, 4.85, P<0.05 or P<0.01), the mRNA expression of TGF-β3 in scar Fbs of the elderly group was significantly lower than that in the young group (t=3.36, P<0.05), and the mRNA expressions of α-SMA in scar Fbs of the two groups were close (t=1.14, P>0.05). The mRNA expressions of ROCK1 and YAP in scar Fbs of the elderly group were significantly higher than those in the young group (t=2.98, 7.60, P<0.05 or P<0.01).  Conclusions  The elderly are prone to scar healing after skin injury. The molecular mechanism may be attributed to the production of extracellular matrix components with higher stiffness, which increases tissue stiffness and thereby activates the expressions of ROCK and YAP/transcriptional co-activator with PDZ-binding motif genes, promoting pro-fibrosis gene and protein expression.

     

  • [1]
    KirkwoodTB. Understanding the odd science of aging[J]. Cell, 2005,120(4):437-447.DOI: 10.1016/j.cell.2005.01.027.
    [2]
    BakerDJ, PetersenRC. Cellular senescence in brain aging and neurodegenerative diseases: evidence and perspectives[J]. J Clin Invest, 2018,128(4):1208-1216. DOI: 10.1172/JCI95145.
    [3]
    RamirezT, LiYM, YinS, et al. Aging aggravates alcoholic liver injury and fibrosis in mice by downregulating sirtuin 1 expression[J]. J Hepatol, 2017,66(3):601-609. DOI: 10.1016/j.jhep.2016.11.004.
    [4]
    ShusterS, BlackMM, Mc VitieE. The influence of age and sex on skin thickness, skin collagen and density[J]. Br J Dermatol, 1975,93(6):639-643. DOI: 10.1111/j.1365-2133.1975.tb05113.x.
    [5]
    MooreAL, MarshallCD, BarnesLA, et al. Scarless wound healing: transitioning from fetal research to regenerative healing[J]. Wiley interdiscip Rev Dev Biol, 2018,7(2): 10.1002/wdev.309. DOI: 10.1002/wdev.309.
    [6]
    WillyardC. Unlocking the secrets of scar-free skin healing[J]. Nature, 2018,563(7732):S86-88. DOI: 10.1038/d41586-018-07430-w.
    [7]
    SolonJ, LeventalI, SenguptaK, et al. Fibroblast adaptation and stiffness matching to soft elastic substrates[J]. Biophys J, 2007,93(12):4453-4461. DOI: 10.1529/biophysj.106.101386.
    [8]
    WellsRG. Tissue mechanics and fibrosis[J]. Biochim Biophys Acta, 2013,1832(7):884-890. DOI: 10.1016/j.bbadis.2013.02.007.
    [9]
    DasguptaI, McCollumD. Control of cellular responses to mechanical cues through YAP/TAZ regulation[J]. J Biol Chem, 2019,294(46):17693-17706. DOI: 10.1074/jbc.REV119.007963.
    [10]
    DupontS. Role of YAP/TAZ in cell-matrix adhesion-mediated signalling and mechanotransduction[J]. Exp Cell Res, 2016,343(1):42-53. DOI: 10.1016/j.yexcr.2015.10.034.
    [11]
    LichtmanMK, Otero-VinasM, FalangaV. Transforming growth factor beta (TGF-β) isoforms in wound healing and fibrosis[J]. Wound Repair Regen, 2016,24(2):215-222. DOI: 10.1111/wrr.12398.
    [12]
    LiuF, LagaresD, ChoiKM, et al. Mechanosignaling through YAP and TAZ drives fibroblast activation and fibrosis[J]. Am J Physiol Lung Cell Mol Physiol, 2015,308(4):L344-357. DOI: 10.1152/ajplung.00300.2014.
    [13]
    SzetoSG, NarimatsuM, LuM, et al. YAP/TAZ are mechano- regulators of TGF-β-Smad signaling and renal fibrogenesis[J]. J Am Soc Nephrol, 2016,27(10):3117-3128.DOI: 10.1681/asn.2015050499.
    [14]
    AbramczykH, ImielaA, Brozek-PluskaB, et al. Advances in Raman imaging combined with AFM and fluorescence microscopy are beneficial for oncology and cancer research[J]. Nanomedicine (Lond), 2019,14(14):1873-1888. DOI: 10.2217/nnm-2018-0335.
    [15]
    BhushanB. Nanotribological and nanomechanical properties of skin with and without cream treatment using atomic force microscopy and nanoindentation[J]. J Colloid Interface Sci, 2012,367(1):1-33. DOI: 10.1016/j.jcis.2011.10.019.
    [16]
    StylianouA, LekkaM, StylianopoulosT. AFM assessing of nanomechanical fingerprints for cancer early diagnosis and classification: from single cell to tissue level[J]. Nanoscale, 2018,10(45):20930-20945. DOI: 10.1039/c8nr06146g.
    [17]
    GauglitzGG, KortingHC, PavicicT, et al. Hypertrophic scarring and keloids: pathomechanisms and current and emerging treatment strategies[J]. Mol Med, 2011,17(1/2):113-125. DOI: 10.2119/molmed.2009.00153.
    [18]
    PuglieseE, CoentroJQ, RaghunathM, et al. Wound healing and scar wars[J]. Adv Drug Deliv Rev, 2018,129:1-3. DOI: 10.1016/j.addr.2018.05.010.
    [19]
    张波, 王正国, 朱佩芳. 皮肤无瘢痕愈合机制的研究进展[J]. 中华烧伤杂志, 2002,18(5):318-320.
    [20]
    KöseO, WaseemA. Keloids and hypertrophic scars: are they two different sides of the same coin?[J]. Dermatol Surg, 2008,34(3):336-346. DOI: 10.1111/j.1524-4725.2007.34067.x.
    [21]
    RittiéL, FisherGJ. Natural and sun-induced aging of human skin[J]. Cold Spring Harb Perspect Med, 2015,5(1):a015370. DOI: 10.1101/cshperspect.a015370.
    [22]
    GeorgesPC, HuiJJ, GombosZ, et al. Increased stiffness of the rat liver precedes matrix deposition: implications for fibrosis[J]. Am J Physiol Gastrointest Liver Physiol, 2007,293(6):G1147-1154. DOI: 10.1152/ajpgi.00032.2007.
    [23]
    Viji BabuPK, RiannaC, BelgeG, et al. Mechanical and migratory properties of normal, scar, and Dupuytren's fibroblasts[J]. J Mol Recognit, 2018,31(9):e2719. DOI: 10.1002/jmr.2719.
    [24]
    TschumperlinDJ, LagaresD. Mechano-therapeutics: targeting mechanical signaling in fibrosis and tumor stroma[J]. Pharmacol Ther, 2020,212:107575. DOI: 10.1016/j.pharmthera.2020.107575.
    [25]
    TschumperlinDJ, LigrestiG, HilscherMB, et al. Mechanosensing and fibrosis[J]. J Clin Invest, 2018,128(1):74-84. DOI: 10.1172/JCI93561.
    [26]
    WuH, YuY, HuangH, et al. Progressive pulmonary fibrosis is caused by elevated mechanical tension on alveolar stem cells[J]. Cell, 2020,180(1):107-121.e17. DOI: 10.1016/j.cell.2019.11.027.
    [27]
    YaoB, ZhuDZ, CuiXL, et al. Modeling human hypertrophic scars with 3D preformed cellular aggregates bioprinting[J]. Bioactive Materials, 2021,In press. DOI: 10.1016/j.bioactmat.2021.09.004.
    [28]
    HinzB. Myofibroblasts[J]. Exp Eye Res, 2016,142:56-70. DOI: 10.1016/j.exer.2015.07.009.
    [29]
    HinzB, McCullochCA, CoelhoNM. Mechanical regulation of myofibroblast phenoconversion and collagen contraction[J]. Exp Cell Res, 2019,379(1):119-128.DOI: 10.1016/j.yexcr.2019.03.027.
    [30]
    KnipeRS, ProbstCK, LagaresD, et al. The Rho kinaseis oforms ROCK1 and ROCK2 each contribute to the development of experimental pulmonary fibrosis[J]. Am J Respir Cell Mol Biol, 2018,58(4):471-481. DOI: 10.1165/rcmb.2017-0075OC.
    [31]
    ShiJ, SurmaM, YangY, et al. Disruption of both ROCK1 and ROCK2 genes in cardiomyocytes promotes autophagy and reduces cardiac fibrosis during aging[J]. FASEB J, 2019,33(6):7348-7362. DOI: 10.1096/fj.201802510R.
    [32]
    ZhouY, HuangX, HeckerL, et al. Inhibition of mechanosensitive signaling in myofibroblasts ameliorates experimental pulmonary fibrosis[J]. J Clin Invest, 2013,123(3):1096-1108. DOI: 10.1172/JCI66700.
    [33]
    NoguchiS, SaitoA, NagaseT. YAP/TAZ signaling as a molecular link between fibrosis and cancer[J]. Int J Mol Sci, 2018,19(11):3674. DOI: 10.3390/ijms19113674.
    [34]
    Picollet-D'hahanN, DolegaME, LiguoriL, et al. A 3D toolbox to enhance physiological relevance of human tissue models[J]. Trends Biotechnol, 2016,34(9):757-769.DOI: 10.1016/j.tibtech.2016.06.012.
    [35]
    YanWC, DavoodiP, VijayavenkataramanS, et al. 3D bioprinting of skin tissue: from pre-processing to final product evaluation[J]. Adv Drug Deliv Rev, 2018,132:270-295.DOI: 10.1016/j.addr.2018.07.016.
    [36]
    朱冬振, 王一惠, 王睿, 等. 外源性肿瘤坏死因子α对三维环境下小鼠间充质干细胞向汗腺细胞分化的影响及机制[J]. 中华烧伤杂志, 2020,36(3):187-194. DOI: 10.3760/cma.j.cn501120-20200105-00005.
  • Relative Articles

    [1]Wang Yunwei, Zhang Hao, Cao Peng, Zhang Wanfu, Tong Lin, Li Shaohui, Chen Yang, Han Chao, Guan Hao. Influences and mechanism of extracellular vesicles from dermal papilla cells of mice on human hypertrophic scar fibroblasts[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2024, 40(3): 258-265. doi: 10.3760/cma.j.cn501225-20231107-00185
    [2]Zhang Lixia, Hu Dahai. Research advances on the role of aerobic glycolysis in skin fibrosis diseases[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2024, 40(4): 389-394. doi: 10.3760/cma.j.cn501225-20230712-00004
    [3]Chen Wei, Xu Guangchao, Huang Zhonglu, Chen Li, Nie Kaiyu. Research advances on the mechanism of nerve regeneration-related protein in skin fibrosis[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2023, 39(5): 491-495. doi: 10.3760/cma.j.cn501225-20220701-00278
    [4]Zhang Qingyi, Zhang Lixia, Han Donghui, Jiao Xiaochun, Zheng Zhao, Guo Kai, Yang Yunshu. Expression of endosialin in human hypertrophic scars and its regulation on fibroblast phenotype[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2023, 39(12): 1168-1174. doi: 10.3760/cma.j.cn501225-20231030-00154
    [5]Lin Shixiu, Guo Bingyu, Hui Qiang, Tao Kai. Effects and mechanism of eleutheroside E on the growth of human hypertrophic scar fibroblasts[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2021, 37(3): 279-287. doi: 10.3760/cma.j.cn501120-20200219-00072
    [6]Guo Bingyu, Lin Feng, Bai Zeming, Tao Kai, Wang Hongyi. Expression of microRNA-296 in rabbit hypertrophic scars and its role to human fibroblasts[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2021, 37(8): 725-730. doi: 10.3760/cma.j.cn501120-20210420-00142
    [7]Wang Zhiyong, Wang Xiqiao, Liu Yingkai, Yuan Bo, Dong Jiaoyun, Song Fei, Jiang Yuzhi, Lu Shuliang. Effects of denatured collagen type Ⅰ on differentiation of human fibroblasts into myofibroblasts[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2018, 34(2): 96-101. doi: 10.3760/cma.j.issn.1009-2587.2018.02.007
    [8]Kuang Fang, Zhang Zhi, Chen Bin, Liu Changling, Zhao Yuanyuan, Xu Zhirong, Li Xiaojian. The expression of SnoN in human hypertrophic scar fibroblasts and the mechanism of its participation in hypertrophic scar formation[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2017, 33(10): 634-638. doi: 10.3760/cma.j.issn.1009-2587.2017.10.011
    [9]Zhang Zhi, Kuang Fang, Liu Changling, Chen Bin, Tang Wenbin, Li Xiaojian. Effects of silencing Smad ubiquitination regulatory factor 2 on the function of human hypertrophic scar-derived fibroblasts[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2017, 33(3): 145-151. doi: 10.3760/cma.j.issn.1009-2587.2017.03.004
    [10]Zhang Lu, Li Haisheng, Yao Zhihui, Yang Sisi, He Weifeng, Wu Jun, Luo Gaoxing. Interaction between P311 and transforming growth factor beta 1 and its effect on the function of murine fibroblasts[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2016, 32(4): 208-215. doi: 10.3760/cma.j.issn.1009-2587.2016.04.005
    [11]Dong Jiaoyun, Song Fei, Liu Yingkai, Wang Xiqiao. Effects of severe hypoxia and low concentration of serum protein on the function of human hypertrophic scar fibroblasts[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2016, 32(10): 594-598. doi: 10.3760/cma.j.issn.1009-2587.2016.10.005
    [12]Wang Yang, Zhang Liangping, Lei Rui, Shen Yichen, Shen Hui, Wu Zhinan, Xu Jinghong. Effects of blocking two sites of transforming growth factor-β/Smads signaling on the formation of scar-related proteins in human skin fibroblasts[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2015, 31(5): 372-377. doi: 10.3760/cma.j.issn.1009-2587.2015.05.013
    [14]LIU Da-en, LI Xuan, ZHANG Guo-you, NIU Zhan-guo, YI Cheng-gang, JIA Yu-bo, XIA Wei, GUO Shu-zhong. Experimental study on effect of hirudin in inhibiting hyperplastic scar fibroblasts[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2009, 25(4): 265-267.
    [15]CAO Chuan, LI Shi-rong, DAI Xia, CHEN Yan-qing, FENG Zhi, QIN Xia, ZHAO Yun, WU Jun. The effects of genistein on tyrosine protein kinase-mitogen activated protein kinase signal transduction pathway in hypertrophic scar fibroblasts[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2008, 24(2): 118-121.
    [16]YANG Ping, WANG Ai-li, LIU De-wu, XU Shun, GU Yao-hui, HUANG Jing, CHEN Bo, LUO Qian-cheng, JIA Qing, WU Zhi-hong. Effect of transfection of recombinant human endothelial nitric oxide synthase gene on hypertrophic scar fibroblasts in vitro[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2008, 24(4): 275-277.
    [17]ZHANG Zhi, LI Xiao-jian, LIANG Da-rong, LI Ye-yang, XU Wei-shi. The antagonistic effect of recombinant human decorin on TGF-β1 stimulation of fibroblasts in collagen lattices of scar[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2006, 22(3): 207-210.
    [18]LIANG Guang-ping, LUO Xiang-dong, YANG Zong-cheng. Influence of human telomerase reverse transcriptase gene transfection on the proliferation of human embryonic fibroblasts[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2005, 21(1): 30-32.
    [19]ZHANG Cheng-de, ZHANG Cai-ping, SONG Lan, LONG Shi-yin, TIAN Ying. An experimental study on the role of protein kinase C in the down-regulation of fibroblast proliferation in normal skin and hyperplastic scar by adrenaline[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2005, 21(6): 448-451.
    [20]JIA Chi-yu. Experimental study on the inhibition of fibroblast contraction by α smooth muscle actin fusion protein[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2004, 20(5): 292-294.
  • Cited by

    Periodical cited type(4)

    1. 侯国清,岳海龙,常倩,马俊涛. 右美托咪定通过转化生长因子-β1途径对大鼠脊柱切除术后硬膜外纤维化的影响. 实用临床医药杂志. 2025(01): 77-82 .
    2. 胡艳阁,丁伟,朱薇. 头皮刃厚皮回植治疗中厚皮供区创面的临床研究. 皖南医学院学报. 2023(01): 40-42 .
    3. 孙佳辰,孙天骏,申传安,赵虹晴,刘馨竹,张熠杰. 衰老皮肤中ⅩⅦ型胶原蛋白α1对表皮干细胞的影响及微小RNA干预机制. 中华烧伤与创面修复杂志. 2022(09): 839-848 . 本站查看
    4. 肖臻阳,林志琥,汪明柱,徐家钦,刘钰,熊武,张熙,周建大. 遵循平衡法则 提高创面修复临床与科研水平. 中国医师杂志. 2021(12): 1761-1763 .

    Other cited types(2)

  • Created with Highcharts 5.0.7Amount of accessChart context menuAbstract Views, HTML Views, PDF Downloads StatisticsAbstract ViewsHTML ViewsPDF Downloads2024-052024-062024-072024-082024-092024-102024-112024-122025-012025-022025-032025-04051015202530
    Created with Highcharts 5.0.7Chart context menuAccess Class DistributionFULLTEXT: 30.0 %FULLTEXT: 30.0 %META: 60.8 %META: 60.8 %PDF: 9.1 %PDF: 9.1 %FULLTEXTMETAPDF
    Created with Highcharts 5.0.7Chart context menuAccess Area Distribution其他: 5.3 %其他: 5.3 %其他: 1.3 %其他: 1.3 %Australia: 0.2 %Australia: 0.2 %China: 1.4 %China: 1.4 %United States: 0.3 %United States: 0.3 %三明: 0.2 %三明: 0.2 %上海: 2.0 %上海: 2.0 %东莞: 0.5 %东莞: 0.5 %临汾: 0.2 %临汾: 0.2 %伊犁: 0.2 %伊犁: 0.2 %佛山: 0.2 %佛山: 0.2 %加利福尼亚州: 0.2 %加利福尼亚州: 0.2 %北京: 2.8 %北京: 2.8 %北方邦: 0.5 %北方邦: 0.5 %南京: 0.5 %南京: 0.5 %南昌: 1.1 %南昌: 1.1 %呼和浩特: 0.2 %呼和浩特: 0.2 %哈尔滨: 0.3 %哈尔滨: 0.3 %哥伦布: 0.5 %哥伦布: 0.5 %商洛: 0.2 %商洛: 0.2 %嘉兴: 0.2 %嘉兴: 0.2 %大同: 0.2 %大同: 0.2 %天津: 0.8 %天津: 0.8 %太原: 0.2 %太原: 0.2 %安庆: 0.6 %安庆: 0.6 %宣城: 0.5 %宣城: 0.5 %密蘇里城: 0.3 %密蘇里城: 0.3 %广州: 1.3 %广州: 1.3 %张家口: 3.0 %张家口: 3.0 %悉尼: 0.2 %悉尼: 0.2 %成都: 0.5 %成都: 0.5 %拉贾斯坦邦: 0.5 %拉贾斯坦邦: 0.5 %昆明: 0.5 %昆明: 0.5 %普赖恩维尔: 0.2 %普赖恩维尔: 0.2 %杭州: 0.6 %杭州: 0.6 %武汉: 0.6 %武汉: 0.6 %沃思堡: 0.3 %沃思堡: 0.3 %沈阳: 0.3 %沈阳: 0.3 %洛阳: 0.2 %洛阳: 0.2 %济南: 1.3 %济南: 1.3 %海得拉巴: 0.5 %海得拉巴: 0.5 %深圳: 1.1 %深圳: 1.1 %温州: 0.2 %温州: 0.2 %漯河: 0.9 %漯河: 0.9 %石家庄: 0.3 %石家庄: 0.3 %绵阳: 1.3 %绵阳: 1.3 %芒廷维尤: 50.2 %芒廷维尤: 50.2 %芜湖: 0.6 %芜湖: 0.6 %芝加哥: 0.3 %芝加哥: 0.3 %苏州: 0.8 %苏州: 0.8 %荆州: 0.5 %荆州: 0.5 %葵涌: 0.3 %葵涌: 0.3 %西宁: 2.7 %西宁: 2.7 %西安: 1.4 %西安: 1.4 %运城: 0.2 %运城: 0.2 %遵义: 0.2 %遵义: 0.2 %邯郸: 0.2 %邯郸: 0.2 %重庆: 6.4 %重庆: 6.4 %银川: 0.5 %银川: 0.5 %长沙: 0.9 %长沙: 0.9 %阜阳: 0.2 %阜阳: 0.2 %青岛: 0.5 %青岛: 0.5 %韶关: 0.2 %韶关: 0.2 %香港: 0.2 %香港: 0.2 %其他其他AustraliaChinaUnited States三明上海东莞临汾伊犁佛山加利福尼亚州北京北方邦南京南昌呼和浩特哈尔滨哥伦布商洛嘉兴大同天津太原安庆宣城密蘇里城广州张家口悉尼成都拉贾斯坦邦昆明普赖恩维尔杭州武汉沃思堡沈阳洛阳济南海得拉巴深圳温州漯河石家庄绵阳芒廷维尤芜湖芝加哥苏州荆州葵涌西宁西安运城遵义邯郸重庆银川长沙阜阳青岛韶关香港

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(8)  / Tables(1)

    Article Metrics

    Article views (386) PDF downloads(58) Cited by(6)
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return