Long term efficacy of absorbable sutures coated with silver nanoparticles on the suture track of skin incisions in mice

He Feng; Du Juan; Song Yanbiao; Ye Mao; Wei Yandong; Liu Xuelai

doi:10.3760/cma.j.cn501225-20231007-00104

Volume 40 Issue 12

Dec. 2024

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Article Navigation > CHINESE JOURNAL OF BURNS AND WOUNDS > 2024 > 40(12): 1184-1192

He F,Du J,Song YB,et al.Long term efficacy of absorbable sutures coated with silver nanoparticles on the suture track of skin incisions in mice[J].Chin J Burns Wounds,2024,40(12):1184-1192.DOI: 10.3760/cma.j.cn501225-20231007-00104.

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Long term efficacy of absorbable sutures coated with silver nanoparticles on the suture track of skin incisions in mice

doi: 10.3760/cma.j.cn501225-20231007-00104

1.
Department of Biochemistry and Immunology, Capital Institute of Pediatrics, Beijing100020, China
2.
Emergency, Critical and Translational Medicine Laboratory, Jilin Province People's Hospital, Changchun130021, China
3.
Central Laboratory, the Second Hospital of Hebei Medical University, Shijiazhuang050061, China
4.
Department of General Surgery, Capital Institute of Pediatrics Affiliated Children Hospital, Beijing100020, China
5.
Department of Neonatal Surgery, Capital Institute of Pediatrics Affiliated Children Hospital, Beijing100020, China

Funds:

General Program of Beijing Natural Science Foundation 7222015

Fund for Beijing Science Technology Development of Traditional Chinese Medicine JJ-2020-50

Research Foundation of Capital Institute of Pediatrics LCYJ-2023-07

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Corresponding author: Liu Xuelai, Email: liuxuelai_steven@163.com
Received Date: 2023-10-07

Abstract

Abstract

Objective To explore the long term efficacy of absorbable sutures coated with silver nanoparticles on the suture track of skin incisions in mice. Methods This study was an experimental research. Eighteen half male and half female BALB/c mice aged 10-12 weeks were divided into normal Vicryl suture group, antibiotic Vicryl suture group, and silver nanoparticle Vicryl suture group according to the random number table, with 6 mice in each group, half male and half female. After the skin incisions were created on the back of all mice, the skin incisions of mice in normal Vicryl suture group, antibiotic Vicryl suture group, and silver nanoparticle Vicryl suture group were performed with continuous intradermal suture by absorbable Vicryl sutures, absorbable Vicryl sutures coated with triclosan antimicrobial, and absorbable Vicryl sutures coated with silver nanoparticles, respectively. On the 25^th day after surgery, the skin tissue in the suture track of incisions was collected, immunohistochemical staining was used to detect the percentages of F4/80, interleukin-6 (IL-6), and tumor necrosis factor α (TNF-α) positive areas in the suture track of incisions of mice, the TdT-mediated dUTP nick-end labeling (TUNEL) staining was used to detect the percentage of TUNEL staining positive area in the suture track of incisions of mice, Masson staining was used to observe the collagen fiber formation in the suture track of incisions of mice, and immunohistochemical staining was used to detect the percentage of tissue cathepsin K positive area in the suture track of incisions of mice. The number of the above experimental samples was 6. Results On the 25^th day after surgery, the percentages of F4/80, IL-6, and TNF-α positive areas in the suture track of incisions of mice in silver nanoparticle Vicryl suture group ((19.2±1.6)%, (20.2±1.7)%, and (16.0±1.6)%) were significantly lower than those in normal Vicryl suture group ((100±6.4)%, (100±7.6)%, and (100±9.6)%) and antibiotic Vicryl suture group ((47.2±3.2)%, (53.8±5.0)%, and 43.2%, P<0.05). The percentages of F4/80, IL-6, and TNF-α positive areas in the suture track of incisions of mice in antibiotic Vicryl suture group were significantly lower than those in normal Vicryl suture group (P<0.05). On the 25^th day after surgery, the percentage of TUNEL staining positive area in the suture track of incisions of mice in silver nanoparticle Vicryl suture group was significantly lower than those in normal Vicryl suture group and antibiotic Vicryl suture group (with both P values<0.05). The percentage of TUNEL staining positive area in the suture track of incisions of mice in antibiotic Vicryl suture group was significantly higher than that in normal Vicryl suture group (P<0.05). On the 25^th day after surgery, the collagen fiber deposition was not obvious in the suture track of incisions of mice in normal Vicryl suture group, no obvious collagen fiber formation was observed in the suture track of incisions of mice in antibiotic Vicryl suture group, while the collagen fibers were mostly densely arranged and well-ordered in the suture track of incisions of mice in silver nanoparticle Vicryl suture group. On the 25^th day after surgery, the percentage of tissue cathepsin K positive area in the suture track of incisions of mice in silver nanoparticle Vicryl suture group was significantly lower than those in normal Vicryl suture group and antibiotic Vicryl suture group (with P values both<0.05), the percentage of tissue cathepsin K positive area in the suture track of incisions of mice in antibiotic Vicryl suture group was significantly lower than that in normal Vicryl suture group (P<0.05). Conclusions In the suture track of incisions of mice, silver nanoparticle Vicryl sutures exhibit superior long-term anti-inflammatory effects compared with absorbable Vicryl sutures coated with triclosan antimicrobial and normal absorbable Vicryl sutures. Additionally, cell apoptosis is reduced, collagen fiber deposition is obvious, and delayed-type hypersensitivity reactions is the weakest in the suture track of incisions of mice sutured by silver nanoparticle Vicryl sutures. The silver nanoparticle Vicryl sutures have potential value for clinical application.
- Wounds and injuries,
- Inflammation,
- Apoptosis,
- Nanoparticles,
- Wound repair

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References

[1]	WongKK,LiuXL.Nanomedicine: a primer for surgeons[J].Pediatr Surg Int,2012,28(10):943-951.DOI: 10.1007/s00383-012-3162-y.
[2]	GaikwadS,BirlaS,IngleAP,et al.Superior in vivo wound-healing activity of mycosynthesized silver nanogel on different wound models in rat[J].Front Microbiol,2022,13:881404.DOI: 10.3389/fmicb.2022.881404.
[3]	ChoudhuryH, PandeyM, LimYQ,et al.Silver nanoparticles: advanced and promising technology in diabetic wound therapy[J].Mater Sci Eng C Mater Biol Appl,2020,112:110925. DOI: 10.1016/j.msec.2020.110925.
[4]	刘雪来,宋岩彪,张创,等.纳米银涂层可吸收线吻合小鼠肠壁近期抗炎疗效的实验研究[J].中国微创外科杂志,2018,18(9):825-829.DOI: 10.3969/j.issn.1009-6604.2018.09.015.
[5]	LiuX,LeePY,HoCM,et al.Silver nanoparticles mediate differential responses in keratinocytes and fibroblasts during skin wound healing[J].ChemMedChem,2010,5(3):468-475.DOI: 10.1002/cmdc.200900502.
[6]	ZhangS,LiuX,WangH,et al.Silver nanoparticle-coated suture effectively reduces inflammation and improves mechanical strength at intestinal anastomosis in mice[J].J Pediatr Surg,2014,49(4):606-613.DOI: 10.1016/j.jpedsurg.2013.12.012.
[7]	ShatanAB, VenclíkováK, ZasońskaBA, et al. Antibacterial silver-conjugated magnetic nanoparticles: design, synthesis and bactericidal effect[J].Pharm Res, 2019,36(10):147. DOI: 10.1007/s11095-019-2680-x.
[8]	DabaghS,HarisSA,IsfahaniBK,et al.Silver-decorated and silica-capped magnetite nanoparticles with effective antibacterial activity and reusability[J].ACS Appl Bio Mater,2023,6(6):2266-2276.DOI: 10.1021/acsabm.3c00122.
[9]	RahmanL,SarwarY,KhaliqS,et al.Surfactin-conjugated silver nanoparticles as an antibacterial and antibiofilm agent against Pseudomonas aeruginosa[J].ACS Appl Mater Interfaces,2023,15(37):43321-43331.DOI: 10.1021/acsami.3c07071.
[10]	JoyaYF,LiuZ,JoyaKS,et al.Preparation and antibacterial properties of laser-generated silver-anatase nanocomposite film against Escherichia coli and Staphylococcus aureus[J].Nanotechnology,2012,23(49):495708.DOI: 10.1088/0957-4484/23/49/495708.
[11]	LeachGA,ChaffinHM,BristyanMC,et al.External knot for running intradermal stitch[J].J Cutan Aesthet Surg,2020,13(1):57-58.DOI: 10.4103/JCAS.JCAS_83_19.
[12]	KonovalovA,TlisovaM,GadzhiagaevV,et al."Unshaved intradermal running suture for elective cranial neurovascular surgeries"[J].World Neurosurg,2023,171:139-143.DOI: 10.1016/j.wneu.2023.01.001.
[13]	刘雪来,宋岩彪,单颖君,等.脱蜡丝线与传统丝线介导腹壁线结周围炎症反应的实验研究[J].发育医学电子杂志,2018,6(3):176-181.DOI: 10.3969/j.issn.2095-5340.2018.03.009.
[14]	刘雪来,宋岩彪,李龙,等.脱蜡丝线线结在小鼠腹壁不同解剖层次诱导早期局部免疫应答的组织学研究[J].发育医学电子杂志,2020,8(2):168-172.DOI: 10.3969/j.issn.2095-5340.2020.02.014.
[15]	严珍珍,王雨翔,张停琳,等.负载银纳米颗粒小球藻的明胶/聚乙二醇水凝胶的性能及其对小鼠全层皮肤缺损感染创面愈合的作用[J].中华烧伤与创面修复杂志,2024,40(1):33-42.DOI: 10.3760/cma.j.cn501225-20231020-00126.
[16]	KehribarL,AydınM,CoşkunHS,et al.Silver nanoparticles enhance the antibacterial effect of antibiotic-loaded bone cement[J].Cureus,2023,15(2):e34992.DOI: 10.7759/cureus.34992.
[17]	ChaiG,WangN,XuM,et al.Poly (vinyl alcohol)/sodium alginate/carboxymethyl chitosan multifunctional hydrogel loading HKUST-1 nanoenzymes for diabetic wound healing[J].Int J Biol Macromol,2024,268(Pt 2):131670.DOI: 10.1016/j.ijbiomac.2024.131670.
[18]	KwanKH,LiuX,ToMK,et al.Modulation of collagen alignment by silver nanoparticles results in better mechanical properties in wound healing[J].Nanomedicine,2011,7(4):497-504.DOI: 10.1016/j.nano.2011.01.003.
[19]	魏添,高凯.硒和硒纳米颗粒在脊髓损伤治疗方面的研究进展[J].国际骨科学杂志,2024,45(3):179-182.DOI: 10.3969/j.issn.1673-7083.2024.03.007.
[20]	PistonesiDB,BelénF,RusoJM,et al.NIR-responsive nano-holed titanium alloy surfaces: a photothermally activated antimicrobial biointerface[J].J Mater Chem B,2024,12(36):8993-9004.DOI: 10.1039/d4tb01307g.
[21]	WongKK,CheungSO,HuangL,et al.Further evidence of the anti-inflammatory effects of silver nanoparticles[J].ChemMedChem,2009,4(7):1129-1135.DOI: 10.1002/cmdc.200900049.
[22]	KumarSSD, RajendranNK, HoureldNN, et al.Recent advances on silver nanoparticle and biopolymer-based biomaterials for wound healing applications[J].Int J Biol Macromol,2018,115:165-175.DOI: 10.1016/j.ijbiomac.2018.04.003.
[23]	ShahA,Ali BuabeidM,ArafaEA,et al.The wound healing and antibacterial potential of triple-component nanocomposite (chitosan-silver-sericin) films loaded with moxifloxacin[J].Int J Pharm,2019,564:22-38.DOI: 10.1016/j.ijpharm.2019.04.046.
[24]	TarushaL,PaolettiS,TravanA,et al.Alginate membranes loaded with hyaluronic acid and silver nanoparticles to foster tissue healing and to control bacterial contamination of non-healing wounds[J].J Mater Sci Mater Med,2018,29(3):22.DOI: 10.1007/s10856-018-6027-7.
[25]	MengX,SunS,GongC,et al.Ag-doped metal-organic frameworks' heterostructure for sonodynamic therapy of deep-seated cancer and bacterial infection[J/OL].ACS Nano,2022(2022-12-30)[2023-10-07]. https://pubmed.ncbi.nlm.nih.gov/36583572/.DOI:10.1021/acsnano.2c08687.[published online ahead of print].
[26]	QiX,HuangY,YouS,et al.Engineering robust Ag-decorated polydopamine nano-photothermal platforms to combat bacterial infection and prompt wound healing[J].Adv Sci (Weinh),2022,9(11):e2106015.DOI: 10.1002/advs.202106015.
[27]	QiaoY,HeJ,ChenW,et al.Light-activatable synergistic therapy of drug-resistant bacteria-infected cutaneous chronic wounds and nonhealing keratitis by cupriferous hollow nanoshells[J].ACS Nano,2020,14(3):3299-3315.DOI: 10.1021/acsnano.9b08930.
[28]	SongGJ,ChoiYS,HwangHS,et al.Silver-composited polydopamine nanoparticles: antibacterial and antioxidant potential in nanocomposite hydrogels[J].Gels,2023,9(3):183.DOI: 10.3390/gels9030183.
[29]	ChangR,ZhaoD,ZhangC,et al.Nanocomposite multifunctional hyaluronic acid hydrogel with photothermal antibacterial and antioxidant properties for infected wound healing[J].Int J Biol Macromol,2023,226:870-884.DOI: 10.1016/j.ijbiomac.2022.12.116.
[30]	WuC,ZhangG,XiaT,et al.Bioinspired synthesis of polydopamine/Ag nanocomposite particles with antibacterial activities[J].Mater Sci Eng C Mater Biol Appl,2015,55:155-165.DOI: 10.1016/j.msec.2015.05.032.
[31]	PhamTN,JiangYS,SuCF,et al.In situ formation of silver nanoparticles-contained gelatin-PEG-dopamine hydrogels via enzymatic cross-linking reaction for improved antibacterial activities[J].Int J Biol Macromol,2020,146:1050-1059.DOI: 10.1016/j.ijbiomac.2019.09.230.
[32]	ZhangK,LuiVCH,ChenY,et al.Delayed application of silver nanoparticles reveals the role of early inflammation in burn wound healing[J].Sci Rep,2020,10(1):6338.DOI: 10.1038/s41598-020-63464-z.
[33]	SaidMM,RehanM,El-SheikhSM,et al.Multifunctional hydroxyapatite/silver nanoparticles/cotton gauze for antimicrobial and biomedical applications[J].Nanomaterials (Basel),2021,11(2):429.DOI: 10.3390/nano11020429.
[34]	BasovA,DzhimakS,SokolovM,et al.Changes in number and antibacterial activity of silver nanoparticles on the surface of suture materials during cyclic freezing[J].Nanomaterials (Basel),2022,12(7):1164.DOI: 10.3390/nano12071164.
[35]	江纯静,杨成雪,喻正文,等.金属离子抗炎作用的分子机制[J].中国组织工程研究,2024,28(10):1626-1633.DOI: 10.12307/2024.267.
[36]	刘雪来.纳米生物载体在实体瘤治疗研究中的应用[J].中华小儿外科杂志,2012,33(9):705-709.DOI: 10.3760/cma.j.issn.0253-3006.2012.09.016.
[37]	WuY,ZhangJ,LinA,et al.Immunomodulatory poly(L-lactic acid) nanofibrous membranes promote diabetic wound healing by inhibiting inflammation, oxidation and bacterial infection[J/OL].Burns Trauma,2024,12:tkae009[2024-11-25]. https://pubmed.ncbi.nlm.nih.gov/38841099/.DOI: 10.1093/burnst/tkae009.
[38]	AlmohamadZ,FahmyR,FaragA,et al.Innovative approach: utilizing silver nanoparticles sheet for improved rabbit cecal anastomosis healing[J].Front Vet Sci,2024,11:1264414.DOI: 10.3389/fvets.2024.1264414.
[39]	ZhaoX,SuS,WuC,et al.High-throughput screening-based design of multifunctional natural polyphenol nano-vesicles to accelerate diabetic wound healing[J].J Nanobiotechnology,2024,22(1):725.DOI: 10.1186/s12951-024-02950-2.
[40]	WangY,ChenC,HeC,et al.Quaternized chitosan-based biomimetic nanozyme hydrogels with ROS scavenging, oxygen generating, and antibacterial capabilities for diabetic wound repair[J].Carbohydr Polym,2025,348(Pt B):122865.DOI: 10.1016/j.carbpol.2024.122865.
[41]	Al-SawareesDK,DarwishRM,Abu-ZuraykR,et al.Assessing silver nanoparticle and antimicrobial combinations for antibacterial activity and biofilm prevention on surgical sutures[J].J Appl Microbiol,2024,135(4):lxae063.DOI: 10.1093/jambio/lxae063.
[42]	LiH,DuanS,LiL,et al.Bio-responsive sliver peroxide-nanocarrier serves as broad-spectrum metallo-β-lactamase inhibitor for combating severe pneumonia[J].Adv Mater,2024,36(11):e2310532.DOI: 10.1002/adma.202310532.
[43]	ZhuJ,WenT,QuS,et al.G-quadruplex/hemin DNAzyme-functionalized silver nanoclusters with synergistic antibacterial and wound healing capabilities for infected wound management[J].Small,2024,20(8):e2307220.DOI: 10.1002/smll.202307220.
[44]	QiM,WangX,ChenJ,et al.Transformation, absorption and toxicological mechanisms of silver nanoparticles in the gastrointestinal tract following oral exposure[J].ACS Nano,2023,17(10):8851-8865.DOI: 10.1021/acsnano.3c00024.
[45]	FuX,RehmanU,WeiL,et al.Silver-dendrimer nanocomposite as emerging therapeutics in anti-bacteria and beyond[J].Drug Resist Updat,2023,68:100935.DOI: 10.1016/j.drup.2023.100935.
[46]	GravanteG,CarusoR,SorgeR,et al.Nanocrystalline silver: a systematic review of randomized trials conducted on burned patients and an evidence-based assessment of potential advantages over older silver formulations[J].Ann Plast Surg,2009,63(2):201-205.DOI: 10.1097/SAP.0b013e3181893825.
[47]	刘雪来,何峰,宋岩彪,等.纳米银薇乔线在小鼠皮内连续缝合线道周围近期抗炎疗效的观察研究[J].发育医学电子杂志,2023,11(4):249-255.DOI: 10.3969/j.issn.2095-5340.2023.04.002.
[48]	LiuX,GaoP,DuJ,et al.Long-term anti-inflammatory efficacy in intestinal anastomosis in mice using silver nanoparticle-coated suture[J].J Pediatr Surg,2017,52(12):2083-2087.DOI: 10.1016/j.jpedsurg.2017.08.026.
[49]	KüpFÖ,ÇoşkunçayS,DumanF.Biosynthesis of silver nanoparticles using leaf extract of Aesculus hippocastanum (horse chestnut): evaluation of their antibacterial, antioxidant and drug release system activities[J].Mater Sci Eng C Mater Biol Appl,2020,107:110207.DOI: 10.1016/j.msec.2019.110207.
[50]	DoescherJ,EmmanuelB,GreveJ,et al.Barbed suture in neck dissection: a randomized clinical study on efficacy, safety and aesthetic outcome[J].Eur Arch Otorhinolaryngol,2024,281(12):6613-6620.DOI: 10.1007/s00405-024-08869-6.
[51]	KamoK, KijimaH, OkuyamaK,et al. Osteolysis of the greater trochanter caused by a foreign body granuloma associated with the Ethibond^® suture after total hip arthroplasty[J].Case Rep Orthop,2017,2017:6082302. DOI: 10.1155/2017/6082302.
[52]	OllivereBJ, BosmanHA, BearcroftPW,et al. Foreign body granulomatous reaction associated with polyethelene 'Fiberwire^®' suture material used in Achilles tendon repair[J].Foot Ankle Surg,2014,20(2):e27-29. DOI: 10.1016/j.fas.2014.01.006.
[53]	OgbechieOA, PaulS, SchalockPC. A technique for identifying vicryl suture hypersensitivity[J].Dermatitis,2014,25(6):370-371. DOI: 10.1097/DER.0000000000000085.