Effects of trehalose gel on full-thickness skin defect wounds in rats and scar hyperplasia in rabbit ears

Jin Jian; Chu Yungao

doi:10.3760/cma.j.cn501225-20240118-00020

Volume 40 Issue 7

Jul. 2024

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Article Navigation > CHINESE JOURNAL OF BURNS AND WOUNDS > 2024 > 40(7): 679-688

Jin J,Chu YG.Effects of trehalose gel on full-thickness skin defect wounds in rats and scar hyperplasia in rabbit ears[J].Chin J Burns Wounds,2024,40(7):679-688.DOI: 10.3760/cma.j.cn501225-20240118-00020.

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Effects of trehalose gel on full-thickness skin defect wounds in rats and scar hyperplasia in rabbit ears

doi: 10.3760/cma.j.cn501225-20240118-00020

Jin Jian¹,
Chu Yungao^{2
,
,}

1.
Department of Polymer Science, Fudan University, Shanghai 200433, China
2.
Shanghai Medical Device Cosmetics Evaluation and Verification Center Medical Device Evaluation Department 2, Shanghai 201802, China

Funds:

Shanghai "Science and Technology Innovation Action Plan" Rising Star Talent Project 23QB1401000

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Corresponding author: Chu Yungao, Email: 358277599@qq.com
Received Date: 2024-01-18

Abstract

Abstract

Objective To investigate the effects of trehalose gel on full-thickness skin defect wounds in rats and scar hyperplasia in rabbit ears. Methods The study was an experimental study. The trehalose gel and carbomer gel were prepared, their appearance after irradiation sterilization, and their physicochemical characterization such as viscosity, film forming rate, bacteria resistance rate, heavy metal content, moisture retention rate, water vapor permeability, sterility, and biocompatibility such as cytotoxicity, intradermal irritation, and sensitization were observed. Thirty male Sprague-Dawley rats aged 8-10 weeks were divided into experimental group, positive control group, and negative control group using a random number table method, with 10 rats in each group. The full-thickness skin defect wound models were prepared on the back of rats in negative control group, positive control group, and experimental group, and were treated with routine dressing change, carbomer gel dressing change, and trehalose gel dressing change, respectively. The wound healing rates on 6 and 12 days after injury and the wound healing time were recorded. On 6 days after injury, the number of autophagosomes and autophagolysosomes in rat wound tissue was detected using transmission electron microscopy. The content of microtubule associated protein light chain 3Ⅰ (LC3Ⅰ) and LC3Ⅱ in rat wound tissue was detected using enzyme-linked immunosorbent assay method and their ratio was calculated. The proportion of type Ⅰ and Ⅲ collagens and their ratio, as well as the total collagen proportion in rat wound tissue were detected using sirius red picric acid staining method. The number of samples in the aforementioned experiments was all 5. Three male New Zealand albino model rabbits aged 3-4 months were taken, and 3 wounds deep to the perichondrium were created on each of the rabbit ears, with six wounds in each group and being grouped and treated as mentioned above. On 30 days after wound healing, the scar tissue of the rabbit ear was observed and evaluated using the Vancouver scar scale. The thickness of the epidermis and dermis in the scar tissue of the rabbit ear was measured using hematoxylin eosin staining, and the proportion of type Ⅰ and Ⅲ collagens and their ratio, as well as the total collagen proportion and arrangement in the scar tissue of the rabbit ear were measured using sirius red picric acid staining method. The number of samples was 6. Results The irradiated trehalose gel and carbomer gel were light yellow and transparent, without odor and impurities. The viscosity, film forming rate, bacteria resistance rate, and moisture retention rate of trehalose gel were significantly better than that of carbomer gel (with t values of 4.13, 3.50, 4.03, and 5.80, respectively, P<0.05), but the water vapor permeability was significantly lower than that of carbomer gel (t=-4.14, P<0.05). No heavy metals or bacteria were detected in any gel. Both of the two gel had no cytotoxicity, and the intradermal irritation and sensitization were negative. On 6 and 12 days after injury, the wound healing rates of rats in positive control group were significantly higher than that in negative control group (with t values of -6.82 and -4.58, respectively, P<0.05); the wound healing rate of rats in experimental group was significantly higher than those in positive control group (with t values of -8.90 and -4.25, respectively, P<0.05) and negative control group (with t values of -8.78 and -4.25, respectively, P<0.05). The wound healing time ((20.4±2.5), (23.4±2.5) d) of rats in positive control group and experimental group was significantly shorter than (27.0±2.1) d in negative control group (with t values of 2.45 and -4.49, respectively, P<0.05). On 6 days after injury, the number of autophagosomes and autophagolysosomes in wound tissue in experimental group of rats were significantly higher than those in positive control group (with t values of 7.37 and 9.33, respectively, P<0.05) and negative control group (with t values of -7.06 and -8.54, respectively, P<0.05). On 6 days after injury, the content of LC3 Ⅱ and LC3 Ⅱ/LC3 Ⅰ in wound tissue in positive control group of rats were significantly higher than that in negative control group (with t values of -4.48 and -2.47, respectively, P<0.05); the content of LC3Ⅰ and LC3 Ⅱ/LC3 Ⅰ in wound tissue in experimental group of rats were significantly higher than those in negative control group (with t values of 11.98 and 6.04, respectively, P<0.05) and positive control group (with t values of -6.64 and -4.17, respectively, P<0.05), the content of LC3Ⅰ was significantly lower than that in negative control group (t=2.33, P<0.05). On 6 days after injury, the proportions of total collagen and type Ⅰ collagen in wound tissue of rats in the three groups were similar, P>0.05. On 6 days after injury, the proportion of type Ⅲ collagen in wound tissue of rats in positive control group was significantly higher than that in negative control group (t=-3.19, P<0.05), and the type Ⅰ collagen/type Ⅲ collagen was significantly lower than that in negative control group (t=2.18, P<0.05); the proportion of type Ⅲ collagen in the wound tissue of rats in experimental group was significantly higher than those in negative control group and positive control group (with t values of -2.38 and 5.91, respectively, P<0.05), and type Ⅰ collagen/type Ⅲ collagen was significantly lower than those in negative control group and positive control group (with t values of 3.08 and -4.35, respectively, P<0.05). On 30 days after wound healing, it was observed that the rabbit ear scar proliferation in positive control group was similar to that in negative control group, while the rabbit ear scar proliferation in experimental group was significantly reduced. On 30 days after wound healing, the color, blood vessels, thickness, hardness score, and total score of rabbit ear scars in experimental group were significantly lower than those in positive control group (with t values of 3.80, 3.80, 2.39, 2.71, and 4.84, respectively, P<0.05) and negative control group (with t values of -3.81, -4.78, 0.04, -2.71, and -5.14, respectively, P<0.05). On 30 days after wound healing, there was no significant difference in the epidermal thickness of rabbit ear scar tissue among experimental group, negative control group, and positive control group (P>0.05); the dermal thickness of rabbit ear scar tissue in positive control group was significantly smaller than that in negative control group (t=5.42, P<0.05), while the dermal thickness of rabbit ear scar tissue in experimental group was significantly smaller than those in negative control group and positive control group (with t values of 11.91 and 8.49, respectively, P<0.05). On 30 days after wound healing, the collagen protein arrangement of scar tissue of rabbits in the three groups was disordered, and the total collagen proportion was similar (P>0.05). The proportion of type Ⅰ collagen of scar tissue in experimental group was significantly lower than that in positive control group (t=3.00, P<0.05), the content of type Ⅲ collagen was significantly higher than those in negative control group and positive control group (with t values of -4.46 and 4.05, respectively, P<0.05), and the type Ⅰcollagen/type Ⅲ collagen was significantly lower than those in negative control group and positive control group (with t values of 8.50 and -5.25, respectively, P<0.05). Conclusions Compared with carbomer gel, trehalose gel has a more suitable physicochemical characterization for wound healing, and has good biocompatibility. It can promote the wound healing of full-thickness defects in rats and reduce scar hyperplasia in rabbit ears based on autophagy activation.
- Biocompatible materials,
- Trehalose,
- Cicatrix,
- Autophagy,
- Wound repair

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References(33)

References

[1]	JinJ, ZhengX, HeF, et al. Therapeutic efficacy of early photobiomodulation therapy on the zones of stasis in burns: an experimental rat model study[J]. Wound Repair Regen, 2018,26(6):426-436. DOI: 10.1111/wrr.12661.
[2]	黄洁, 李书原, 王雪欣, 等. 我国烧伤专业医护人员对Ⅱ度烧伤创面的早期处理的横断面调查与分析[J]. 中华烧伤与创面修复杂志, 2022, 38(6):538-548. DOI: 10.3760/cma.j.cn501225-20220317-00065.
[3]	史春梦. 加强难愈合创面间充质干细胞治疗的基础与转化研究[J].中华烧伤与创面修复杂志,2022,38(11):999-1003. DOI: 10.3760/cma.j.cn501225-20220913-00405.
[4]	JinJ, LiH, ChenZ, et al. 3-D wound scanner: a novel, effective, reliable, and convenient tool for measuring scar area[J]. Burns, 2018,44(8):1930-1939. DOI: 10.1016/j.burns.2018.05.009.
[5]	苏滢泓, 夏文政, 黄昕, 等. 糖皮质激素治疗瘢痕疙瘩的研究进展[J].中华烧伤与创面修复杂志,2023,39(9):886-890. DOI: 10.3760/cma.j.cn501225-20230602-00198.
[6]	王蕴璋, 苏晨, 付思祺, 等. 瘢痕疙瘩中的成纤维细胞特性研究进展[J].中华烧伤与创面修复杂志,2022,38(6):590-594. DOI: 10.3760/cma.j.cn501120-20210510-00176.
[7]	MijaljicaD, SpadaF, KlionskyDJ, et al. Autophagy is the key to making chronic wounds acute in skin wound healing[J]. Autophagy, 2023,19(9):2578-2584. DOI: 10.1080/15548627.2023.2194155.
[8]	CuiD, WangZ, DangQ, et al. Spliceosome component Usp39 contributes to hepatic lipid homeostasis through the regulation of autophagy[J]. Nat Commun, 2023,14(1):7032. DOI: 10.1038/s41467-023-42461-6.
[9]	WardMA, VangalaJR, KamberKaya HE, et al. Transcription factor Nrf1 regulates proteotoxic stress-induced autophagy[J]. J Cell Biol, 2024,223(6):e202306150.DOI: 10.1083/jcb.202306150.
[10]	JinJ, ZhuKS, TangSM, et al. Trehalose promotes functional recovery of keratinocytes under oxidative stress and wound healing via ATG5/ATG7[J]. Burns, 2023,49(6):1382-1391. DOI: 10.1016/j.burns.2022.11.014.
[11]	LiaoD, WeiS, HuJ. Inhibition of miR-542-3p augments autophagy to promote diabetic corneal wound healing[J]. Eye Vis (Lond), 2024,11(1):3. DOI: 10.1186/s40662-023-00370-1.
[12]	RenH, ZhaoF, ZhangQ, et al. Autophagy and skin wound healing[J/OL]. Burns Trauma, 2022,10:tkac003[2024-01-18]. https://pubmed.ncbi.nlm.nih.gov/35187180/.DOI: 10.1093/burnst/tkac003.
[13]	YaoS, PengS, WangX. Phospholipase Dε interacts with autophagy-related protein 8 and promotes autophagy in Arabidopsis response to nitrogen deficiency[J]. Plant J, 2022,109(6):1519-1534. DOI: 10.1111/tpj.15649.
[14]	JakowecNA, FineganM, FinkelSE. Disruption of trehalose periplasmic recycling dysregulates cAMP-CRP signaling in Escherichia coli during stationary phase[J]. J Bacteriol, 2023,205(11):e0029223. DOI: 10.1128/jb.00292-23.
[15]	LuS, HarunariE, OkuN, et al. Trehangelin E, a bisacyl trehalose with plant growth promoting activity from a rare actinomycete Polymorphospora sp. RD064483[J]. J Antibiot (Tokyo), 2022,75(5):296-300. DOI: 10.1038/s41429-022-00519-5.
[16]	谢贵会, 李冰, 蔡美娟, 等. TFEB在海藻糖改善哮喘小鼠肺脏炎症中的作用研究[J].中国免疫学杂志,2022,38(12):1439-1444. DOI: 10.3969/j.issn.1000-484X.2022.12.006.
[17]	周立敏, 房贤文. 海藻糖和深海鱼类胶原蛋白防护UVA照射皮肤损伤的动物实验[J].中国皮肤性病学杂志,2009,23(2):79-80.
[18]	贾晓明, 蔡宏. 皮肤低温损伤机制中海藻糖的抗氧自由基作用[J].军医进修学院学报,2005,26(6):435-436. DOI: 10.3969/j.issn.1005-1139.2005.06.014.
[19]	王利江, 庄立琨, 杨通旺, 等. 海藻糖对肝脏缺血再灌注损伤的保护作用及机制研究[J].中华器官移植杂志,2021,42(2):109-115. DOI: 10.3760/cma.j.cn421203-20191022-00380.
[20]	WangXL, LiZC, ZhangC, et al. Spring Viremia of Carp Virus N Protein Negatively Regulates IFN Induction through Autophagy-Lysosome-Dependent Degradation of STING[J]. J Immunol, 2023,210(1):72-81. DOI: 10.4049/jimmunol.2200477.
[21]	Bushra, MahaIF, YuY, et al. Effects of autophagy inhibition by 3-methyladenine on encystation, morphology, and metabolites of Cryptocaryon irritans[J]. Parasitol Res, 2023,122(2):509-517. DOI: 10.1007/s00436-022-07751-w.
[22]	BenaroudjN, LeeDH, GoldbergAL. Trehalose accumulation during cellular stress protects cells and cellular proteins from damage by oxygen radicals[J]. J Biol Chem, 2001,276(26):24261-24267. DOI: 10.1074/jbc.M101487200.
[23]	FernandezO, BéthencourtL, QueroA, et al. Trehalose and plant stress responses: friend or foe?[J]. Trends Plant Sci, 2010,15(7):409-417. DOI: 10.1016/j.tplants.2010.04.004.
[24]	ThakralS, SonjeJ, MunjalB, et al. Mannitol as an Excipient for Lyophilized Injectable Formulations[J]. J Pharm Sci, 2023,112(1):19-35. DOI: 10.1016/j.xphs.2022.08.029.
[25]	吴晓娜,汪宜宇,赵凯.MXene基复合水凝胶在修复感染创面中的研究进展[J].复合材料学报, 2024, 41(7):3431-3445.DOI: 10.13801/j.cnki.fhclxb.20231214.001.
[26]	AnsariM, MeftahizadehH, EslamiH. Physical and antibacterial properties of Chitosan-guar-peppermint gel for improving wound healing[J]. Polymer Bulletin, 2022, 80(7):8133-8149. DOI: 10.1007/s00289-022-04448-z.
[27]	胡香敏, 刘大猛. 基于差分反射高光谱成像的薄层TMDC材料检测技术研究[J].光散射学报,2022,34(1):60-65.DOI: 10.13883/j.issn1004-5929.202201011.
[28]	米思聪, 焦瑶, 郭力嘉, 等. 聚赖氨酸凝胶治疗大鼠实验性牙周炎的效果观察[J].北京口腔医学,2023,31(3):168-171. DOI: 10.20049/j.bjkqyx.1006-673X.2023.03.004.
[29]	卢翰生, 陈颖, 冯芷媚, 等. 水凝胶敷料水蒸气透过性能的检测与思考[J].中国医疗器械信息,2023,29(9):27-29,41. DOI: 10.3969/j.issn.1006-6586.2023.09.008.
[30]	ChuY, FangY, WuH, et al. Establishment and characterization of immortalized human vocal fold fibroblast cell lines[J]. Biotechnol Lett, 2023,45(3):347-355. DOI: 10.1007/s10529-023-03350-6.
[31]	ChenZ, HuX, LinZ, et al. Layered GelMA/PEGDA hydrogel microneedle patch as an intradermal delivery system for hypertrophic scar treatment[J]. ACS Appl Mater Interfaces, 2023,15(37):43309-43320. DOI: 10.1021/acsami.3c06800.
[32]	GotoH, ArimaT, TakahashiA, et al. Trimebutine prevents corneal inflammation in a rat alkali burn model[J]. Sci Rep, 2024,14(1):12111. DOI: 10.1038/s41598-024-61112-4.
[33]	WihastyokoHYL, SoehartoS, WidjajantoE, et al. Modification of the Vancouver Scar Scale (VSS) score for scarring assessment using rattus novergicus abnormal scar model[J]. RJPT, 2022(3):15. DOI: 10.52711/0974-360X.2022.00219.

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Effects of trehalose gel on full-thickness skin defect wounds in rats and scar hyperplasia in rabbit ears

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Effects of trehalose gel on full-thickness skin defect wounds in rats and scar hyperplasia in rabbit ears

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