Email Alerts
RSS
Volume 41 Issue 9
Sep.  2025
Turn off MathJax
Article Contents
Article Navigation >  CHINESE JOURNAL OF BURNS AND WOUNDS  > 2025  >  41(9): 887-894
Wang X,Cheng W,Liu XJ,et al.Effects of microneedle injection of rat epidermal stem cells on wound healing of full-thickness skin defects in rats[J].Chin J Burns Wounds,2025,41(9):887-894.DOI: 10.3760/cma.j.cn501225-20241031-00427.
Citation: Wang X,Cheng W,Liu XJ,et al.Effects of microneedle injection of rat epidermal stem cells on wound healing of full-thickness skin defects in rats[J].Chin J Burns Wounds,2025,41(9):887-894.DOI: 10.3760/cma.j.cn501225-20241031-00427.
shu

Effects of microneedle injection of rat epidermal stem cells on wound healing of full-thickness skin defects in rats

doi: 10.3760/cma.j.cn501225-20241031-00427
  • 1.

    Department of Burn Plastic Surgery, the Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou 215000, China

  • 2.

    School of Physical Science and Technology, Jiangsu Key Laboratory of Thin Film Materials, Soochow University, Suzhou 215006, China

Funds:

Suzhou Special Project for Diagnosis and Treatment of Key Clinical Diseases LCZX202315

Suzhou Science and Technology Bureau-Medical Devices and New Medicine Project SLJ2021018

More Information
    Abstract
  •   Objective  To investigate the effects of microneedle injection of rat epidermal stem cells (ESCs) on wound healing of full-thickness skin defects in rats.  Methods  This study was an experimental study. The hollow silicon-based microneedle injection device was developed successfully by utilizing laser-etching technology. ESCs were isolated and cultured from the skin of two 2-day-old female Sprague-Dawley rats and identified successfully by using flow cytometry and immunofluorescence method. A total of 18 eight-week-old female Sprague-Dawley rats were divided into control group, spray group, and microneedle group according to the random number table method, with 6 rats in each group. All rats were inflicted with a full-thickness skin defect wound on the dorsum. On day 0 (immediately after injury), the wounds in control group and spray group were sprayed with 0.5 mL phosphate-buffered solution and 0.5 mL rat ESCs suspension, respectively, and the wounds in microneedle group of rats were injected with 0.5 mL rat ESCs suspension through the hollow silicon-based microneedle injection device. On days 3, 7, and 14 after injury, the wound healing was observed and the wound healing rate was calculated. On day 14 after injury, the wound tissue of rats were harvested for hematoxylin and eosin staining to observe the epithelialization of wounds, for Masson staining to detect the collagen fiber content in wound tissue, and for immunohistochemical staining to detect the neovascular number in wound tissue.  Results  Within 14 days after injury, the wounds in three groups of rats progressively healed. On day 3 after injury, there was no statistically significant difference in the overall comparison of the wound healing rate among the three groups of rats (P>0.05). On days 7 and 14 after injury, the wound healing rates in microneedle group of rats were (77.0±4.6)% and (95.0±2.1)%, respectively, which were significantly higher than (66.5±8.6)% and (88.3±3.1)% in spray group and (44.5±5.7)% and (78.8±6.3)% in control group (P<0.05); the wound healing rates in spray group of rats were significantly higher than those in control group (P<0.05). On day 14 after injury, the degree of wound epithelialization in the microneedle group of rats was higher than that in spray group and control group, and hair follicles developed in the newly regenerated skin tissue. On day 14 after injury, the collagen fiber content in the wound tissue in microneedle group and spray group of rats was significantly higher than that in control group (P<0.05), and the collagen fiber content in the wound tissue in microneedle group of rats was significantly higher than that in spray group (P<0.05); the neovascular number in the wound tissue in microneedle group and spray group of rats was significantly more than that in control group (P<0.05), and the neovascular number in the wound tissue in microneedle group of rats was significantly more than that in spray group (P<0.05).  Conclusions  ESCs can promote neovascularization and collagen fiber formation in the wound tissue, thereby accelerating the wound healing and improve the quality of wound healing in rats with full-thickness skin defects. Injecting rat ESCs suspension through hollow silicon-based microneedle injection device shows better wound healing effects than using conventional approaches, making it an effective cell delivery method.

     

    • FullText(HTML)
  • loading
    • References(40)
  • [1]
    董鸿斐,黄茜,李先慧,等.胎盘间充质干细胞促进大鼠急性皮肤创面修复[J].中国组织工程研究,2024,28(13):2047-2053.DOI: 10.12307/2024.136.
    [2]
    LoughDM,YangM,BlumA,et al.Transplantation of the LGR6+ epithelial stem cell into full-thickness cutaneous wounds results in enhanced healing, nascent hair follicle development, and augmentation of angiogenic analytes[J].Plast Reconstr Surg,2014,133(3):579-590.DOI: 10.1097/PRS.0000000000000075.
    [3]
    YangY, HuangJ, ZengA, et al. The role of the skin microbiome in wound healing[J/OL]. Burns Trauma, 2024, 12:tkad059[2024-10-31]. https://pubmed.ncbi.nlm.nih.gov/38444635/. DOI: 10.1093/burnst/tkad059.
    [4]
    JamesSE, BoothS, DheansaB, et al. Sprayed cultured autologous keratinocytes used alone or in combination with meshed autografts to accelerate wound closure in difficult-to-heal burns patients[J]. Burns, 2010, 36(3):e10-20. DOI: 10.1016/j.burns.2008.11.011.
    [5]
    JacksonCJ,TønsethKA,UtheimTP.Cultured epidermal stem cells in regenerative medicine[J].Stem Cell Res Ther,2017,8(1):155.DOI: 10.1186/s13287-017-0587-1.
    [6]
    ChengD,ZhuX,YanS,et al.New insights into inflammatory memory of epidermal stem cells[J].Front Immunol,2023,14:1188559.DOI: 10.3389/fimmu.2023.1188559.
    [7]
    YangRH,QiSH,ShuB,et al.Epidermal stem cells (ESCs) accelerate diabetic wound healing via the Notch signalling pathway[J].Biosci Rep,2016,36(4):e00364.DOI: 10.1042/BSR20160034.
    [8]
    石志远,张博涵,孙佳辰,等. 表皮干细胞在皮肤创面修复中的作用与机制研究进展[J]. 中华烧伤与创面修复杂志,2022,38(9):854-858. DOI: 10.3760/cma.j.cn501120-20211109-00382.
    [9]
    GaoZ,ShengT,ZhangW,et al.Microneedle-mediated cell therapy[J].Adv Sci (Weinh),2024,11(8):e2304124.DOI: 10.1002/advs.202304124.
    [10]
    LeeK,XueY,LeeJ,et al.A patch of detachable hybrid microneedle depot for localized delivery of mesenchymal stem cells in regeneration therapy[J].Adv Funct Mater,2020,30(23):2000086.DOI: 10.1002/adfm.202000086.
    [11]
    SumathyB,VelayudhanS.Fabrication and evaluation of a bi-layered gelatin based scaffold with arrayed micro-pits for full-thickness skin construct[J].Int J Biol Macromol,2023,251:126360.DOI: 10.1016/j.ijbiomac.2023.126360.
    [12]
    WeidnerN,FolkmanJ,PozzaF,et al.Tumor angiogenesis: a new significant and independent prognostic indicator in early-stage breast carcinoma[J].J Natl Cancer Inst,1992,84(24):1875-1887.DOI: 10.1093/jnci/84.24.1875.
    [13]
    PersaOD,KoesterJ,NiessenCM.Regulation of cell polarity and tissue architecture in epidermal aging and cancer[J].J Invest Dermatol,2021,141(4S):1017-1023.DOI: 10.1016/j.jid.2020.12.012.
    [14]
    PeñaOA,MartinP.Cellular and molecular mechanisms of skin wound healing[J].Nat Rev Mol Cell Biol,2024,25(8):599-616.DOI: 10.1038/s41580-024-00715-1.
    [15]
    HuangS,KuriP,AubertY,et al.Lgr6 marks epidermal stem cells with a nerve-dependent role in wound re-epithelialization[J].Cell Stem Cell,2021,28(9):1582-1596.e6. DOI: 10.1016/j.stem.2021.05.007.
    [16]
    ZhangM,HuangM,DongX,et al.Rotating cell culture system-induced injectable self-assembled microtissues with epidermal stem cells for full-thickness skin repair[J].PeerJ,2024,12:e18418.DOI: 10.7717/peerj.18418.
    [17]
    KangD,WangX,ChenW,et al.Epidermal stem cell-derived exosomes improve wound healing by promoting the proliferation and migration of human skin fibroblasts[J/OL].Burns Trauma,2024,12:tkae047[2024-10-31].https://pubmed.ncbi.nlm.nih.gov/39687464/. DOI: 10.1093/burnst/tkae047.
    [18]
    SzaboAZ,FongS,YueL,et al.The CD44+ ALDH+ population of human keratinocytes is enriched for epidermal stem cells with long-term repopulating ability[J].Stem Cells,2013,31(4):786-799.DOI: 10.1002/stem.1329.
    [19]
    ShiY,YangR,TuL,et al.Long non-coding RNA HOTAIR promotes burn wound healing by regulating epidermal stem cells[J].Mol Med Rep,2020,22(3):1811-1820.DOI: 10.3892/mmr.2020.11268.
    [20]
    黄韶斌,胡志成,张逸,等.异体表皮干细胞对裸鼠全层皮肤缺损创面移植异体全厚皮成活的影响及其机制[J].中华烧伤杂志,2021,37(11):1061-1069.DOI: 10.3760/cma.j.cn501120-20200704-00339.
    [21]
    HuZ,ChenY,GaoM,et al.Novel strategy for primary epithelial cell isolation: combination of hyaluronidase and collagenase I[J].Cell Prolif,2022,56(1):e13320.DOI: 10.1111/cpr.13320.
    [22]
    SuzukiY,YanagisawaM,YagiH,et al.Involvement of beta1-integrin up-regulation in basic fibroblast growth factor- and epidermal growth factor-induced proliferation of mouse neuroepithelial cells[J].J Biol Chem,2010,285(24):18443-18451.DOI: 10.1074/jbc.M110.114645.
    [23]
    JinY, LiS, YuQ, et al. Application of stem cells in regeneration medicine[J]. MedComm (2020), 2023, 4(4):e291. DOI: 10.1002/mco2.291.
    [24]
    HuangS,HuZ,WangP,et al.Rat epidermal stem cells promote the angiogenesis of full-thickness wounds[J].Stem Cell Res Ther,2020,11(1):344.DOI: 10.1186/s13287-020-01844-y.
    [25]
    KucharzewskiM,RojczykE,Wilemska-KucharzewskaK,et al.Novel trends in application of stem cells in skin wound healing[J].Eur J Pharmacol,2019,843:307-315.DOI: 10.1016/j.ejphar.2018.12.012.
    [26]
    LiuM,LiuZ,ChenY,et al.Dendritic epidermal T cells secreting exosomes promote the proliferation of epidermal stem cells to enhance wound re-epithelialization[J].Stem Cell Res Ther,2022,13(1):121.DOI: 10.1186/s13287-022-02783-6.
    [27]
    DonnellyRF,SinghTR,TunneyMM,et al.Microneedle arrays allow lower microbial penetration than hypodermic needles in vitro[J].Pharm Res,2009,26(11):2513-2522.DOI: 10.1007/s11095-009-9967-2.
    [28]
    刘颖,程凤,王泽薇,等.负载小鼠脂肪干细胞的甲壳素/透明质酸/胶原水凝胶的制备及其对大鼠全层皮肤缺损创面愈合的作用[J].中华烧伤与创面修复杂志,2024,40(1):50-56.DOI: 10.3760/cma.j.cn501225-20230928-00101.
    [29]
    林志骁,张玉恒,黄容,等.负载大鼠表皮干细胞的聚己内酯-乙酸纤维素纳米纤维支架对大鼠全层皮肤缺损创面愈合的影响及其机制[J].中华烧伤杂志,2021,37(5):460-468.DOI: 10.3760/cma.j.cn501120-20210104-00005.
    [30]
    CaoH,DuanL,ZhangY,et al.Current hydrogel advances in physicochemical and biological response-driven biomedical application diversity[J]. Signal Transduct Target Ther,2021,6(1):426.DOI: 10.1038/s41392-021-00830-x.
    [31]
    XiaoT, HeG, GengH, et al. Microneedle assisted delivery of epidermal and dermal stem cells to promote hair growth[J]. J Mater Sci, 2024, 59(19): 8427-8440.DOI: 10.1007/s10853-024-09592-7.
    [32]
    ZhaoX, WuZ, GuoY, et al. Cell-laden biomimetic microneedles reconstruct skin rete ridge and stem cell niche[J]. J Nanobiotechnology, 2025, 23(1): 415. DOI: 10.1186/s12951-025-03430-x.
    [33]
    ZhaoY, WangM, LiangF, et al. Recent strategies for enhancing the therapeutic efficacy of stem cells in wound healing[J]. Stem Cell Res Ther, 2021, 12(1): 588. DOI: 10.1186/s13287-021-02657-3.
    [34]
    ZhangY,ChenZ,WuW,et al.Focused ion beam (FIB) prepared microtextured microneedle array capable of delivering drugs through punctured skin[J].Sci Rep,2024,14(1):31489. DOI: 10.1038/s41598-024-83094-z.
    [35]
    WuJ, LiX, YanX, et al. Fabrication of self-powered silicon microneedles for transdermal drug delivery[J]. J Phy: Conf Ser, 2024, 2884: 012004.DOI: 10.1088/1742-6596/2884/1/012004.
    [36]
    Pradeep NarayananS, RaghavanS. Solid silicon microneedles for drug delivery applications[J]. Int J Adv Manuf Technol, 2017, 93(1/2/3/4): 407-422.DOI: 10.1007/s00170-016-9698-6.
    [37]
    邹帅苏晓东程微等具有复合结构的硅微针中国CN202120900036.02021-12-032024-10-31https://cprs.patentstar.com.cn/Search/Detail?ANE=9CGB3CBA9IAB9FIF9GCB9IBA9GAG9IFE9BIA9EIDAGIADIGA

    邹帅,苏晓东,程微,等.具有复合结构的硅微针:中国,CN202120900036.0[P].2021-12-03[2024-10-31]. https://cprs.patentstar.com.cn/Search/Detail?ANE=9CGB3CBA9IAB9FIF9GCB9IBA9GAG9IFE9BIA9EIDAGIADIGA.
    [38]
    ChengW,WangX,ZouS,et al.Fabrication of black silicon microneedle arrays for high drug loading[J].J Funct Biomater,2023,14(5):245.DOI: 10.3390/jfb14050245.
    [39]
    ŚcieżyńskaA,NogowskaA,SikorskaM,et al.Isolation and culture of human primary keratinocytes-a methods review[J].Exp Dermatol,2019,28(2):107-112.DOI: 10.1111/exd.13860.
    [40]
    ZhangS,YangL,LiuJ,et al.Microneedle systems: cell, exosome, and nucleic acid based strategies[J].Biomater Sci,2023,11(21):7018-7033.DOI: 10.1039/d3bm01103h.
    • Relative Articles
    • Supplements(0)
    • Cited By
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(6)  / Tables(2)

    Get Citation
    PDF
    XML

    Article Metrics

    Article views (215) PDF downloads(8) Cited by()
    Proportional views
    Related

    Copyright © Chinese Journal of Burns京ICP备07035254号-14

    E-mail：shaoshangzazhi@163.com

    Website：https://zhsszz.xml-journal.net/

    Supported by: Beijing Renhe Information Technology Co. Ltd    

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return