Indicative effect of indocyanine green-near-infrared fluorescence imaging in the thickness of necrotic dermal tissue in porcine burn wounds

Zhang Yushen; Pan Xiang; Ge Yunlong; Yue Junchen; Song Yixuan; Song Weiye; Zhao Ran

doi:10.3760/cma.j.cn501225-20250731-00337

Volume 41 Issue 11

Nov. 2025

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Article Navigation > CHINESE JOURNAL OF BURNS AND WOUNDS > 2025 > 41(11): 1074-1082

Zhang YS,Pan X,Ge YL,et al.Indicative effect of indocyanine green-near-infrared fluorescence imaging in the thickness of necrotic dermal tissue in porcine burn wounds[J].Chin J Burns Wounds,2025,41(11):1074-1082.DOI: 10.3760/cma.j.cn501225-20250731-00337.

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Indicative effect of indocyanine green-near-infrared fluorescence imaging in the thickness of necrotic dermal tissue in porcine burn wounds

doi: 10.3760/cma.j.cn501225-20250731-00337

1.
Department of Burn and Plastic Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, China
2.
National Key Laboratory of Advanced Equipment and Technology for Metal Forming, School of Mechanical Engineering, Shandong University, Jinan 250061, China
3.
College of Aerospace Science and Engineering, National University of Defense Technology, Changsha 410073, China

Funds:

Youth Science Fund Project of National Natural Science Foundation of China 82402897

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Corresponding author: Zhao Ran, Email: zhaoranburn@126.com
Received Date: 2025-07-31

Abstract

Abstract

Objective To explore the indicative effects of indocyanine green-near-infrared fluorescence imaging (ICG-NIFI) in the thickness of necrotic dermal tissue in porcine burn wounds. Methods This study was a medical-engineering interdisciplinary basic research. One 3-month-old male Bama miniature pig was selected, and circular burn wounds with a diameter of 2 cm were created by applying a scalding instrument with inflicting temperature of 75 ℃ to the skin on the dorsal side of the pig for 3, 7, and 11 seconds, respectively, with two wounds for each duration of injury. The fluorescence intensity of the regions of interest in the wounds and the surrounding normal skin was detected by ICG-NIFI technology within 700 seconds after injecting indocyanine green (hereinafter referred to as after administration). The trend of the normalized fluorescence intensity was observed, and the time when the fluorescence intensity of the regions of interest in the wounds and the surrounding normal skin reached the peak (hereinafter referred to as the peak time) was determined. Additional two 3-month-old male Bama miniature pigs were selected, and circular burn wounds with a diameter of 2 cm were created on the skin of bilateral thoracic walls by applying a scalding instrument with inflicting temperature of 75 ℃ for 5, 7, 9, 11, 13, 15, 17, and 19 seconds, respectively, with 4 wounds for each duration of injury. The blood flow intensity of the regions of interest in the wounds was detected by laser speckle contrast imaging technology; the relative fluorescence intensity of the regions of interest in the wounds was detected by ICG-NIFI technology at the peak time of the wounds and the surrounding normal skin. The full-thickness skin tissue of the wounds was taken for hematoxylin-eosin staining, and the thickness of necrotic dermal tissue was measured. The correlation between the blood flow intensity of the regions of interest in the wounds and the relative fluorescence intensity at the peak time of the regions of interest in the wounds and the surrounding normal skin and the thickness of necrotic dermal tissue in the wounds was analyzed. Results The fluorescence intensity of the regions of interest in the surrounding normal skin showed a rapid increase followed by a slow decrease, with the peak time of approximately 60 seconds after administration. The fluorescence intensity of the regions of interest in the wounds showed a slow increase followed by a sustained stable trend, with the peak time of approximately 600 seconds after administration. The thickness of necrotic dermal tissue in the wounds caused by durations of injury of 5, 7, 9, 11, 13, 15, 17, and 19 seconds was (101±8), (130±6), (201±19), (197±30), (204±21), (280±39), (302±35), and (366±27) μm, respectively. The correlation between the blood flow intensity of the regions of interest in the wounds and the thickness of necrotic dermal tissue in the wounds was not significant (P>0.05). The relative fluorescence intensity of the regions of interest in the wounds at 60 seconds after administration was significantly negatively correlated with the thickness of necrotic dermal tissue in the wounds (R²=0.97, P<0.05), and the relative fluorescence intensity of the regions of interest in the wounds at 600 seconds after administration was significantly positively correlated with the thickness of necrotic dermal tissue in the wounds (R²=0.96, P<0.05). Conclusions The relative fluorescence intensity of the regions of interest in porcine burn wounds detected by ICG-NIFI at 60 and 600 seconds after administration was significantly correlated with the thickness of necrotic dermal tissue in the wounds, indicating that ICG-NIFI has a significantly sensitive indicative effects for the thickness of necrotic dermal tissue in porcine burn wounds.
- Burns,
- Dermis,
- Laser speckle contrast imaging,
- Laser-Doppler flowmetry,
- Fluorescence imaging,
- Indocyanine green

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References(41)

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