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Article Navigation >  CHINESE JOURNAL OF BURNS AND WOUNDS  >  > Accepted Manuscript
Su Sen,Liu Xiaoyan,Zhang Ting,et al.Study on temporal changes in energy and material metabolism in burned mice[J].Chin J Burns Wounds,2025,41(12):1-11.DOI: 10.3760/cma.j.cn501225-20250807-00349.
Citation: Su Sen,Liu Xiaoyan,Zhang Ting,et al.Study on temporal changes in energy and material metabolism in burned mice[J].Chin J Burns Wounds,2025,41(12):1-11.DOI: 10.3760/cma.j.cn501225-20250807-00349.
shu

Study on temporal changes in energy and material metabolism in burned mice

doi: 10.3760/cma.j.cn501225-20250807-00349
  • 1.

    Clinical Medical Research Center, the First Affiliated Hospital of Army Medical University (the Third Military Medical University), Chongqing 400038

  • 2.

    Clinical Medical Research Center, State Key Laboratory of Trauma and Chemical Poisoning, the First Affiliated Hospital of Army Medical University (the Third Military Medical University), Chongqing 400038, China

Funds:

General Program of National Natural Science Foundation of China 82472549

General Program of Natural Science Foundation of Chongqing CSTB2024NSCQ-MSX0031

Key Research Project of State Key Laboratory of Trauma and Chemical Poisoning 2024K003

More Information
  • Corresponding author: Peng Xi, Email: pxlrmm@tmmu.edu.cn
  • Received Date: 2025-08-07
    Available Online: 2025-12-11
    Abstract
  •   Objective  To investigate the temporal dynamics of energy metabolism and material metabolism in burned mice.  Methods  This study was an experimental study. Sixteen male C57BL/6N mice aged 8-10 weeks were randomly assigned using a random number table to sham injury group (n=8) treated with sham injury and burn group (n=8) treated with scald injury. From days 1 to 14 post-burn, the daily water intake, food intake, activity levels, energy expenditure, and resting energy expenditure (REE) of mice were dynamically monitored using a small animal metabolic monitoring system. Daily body weight changes and respiratory entropy from days 1 to 14 post-burn, cumulative water intake, food intake, and activity levels from days 1 to 14 post-burn, respiratory entropy from days 1 to 7 post-burn, energy expenditure on days 1, 3, 7, 10, and 14 post-burn, and nighttime carbohydrate and fat oxidation rates on days 1, 3, 7, 10, and 14 post-burn were calculated. Forty-eight male C57BL/6N mice aged 8-10 weeks were randomly assigned using a random number table: 8 mice received sham injury, and the remaining 40 mice underwent scald injury. Cardiac blood samples were collected from burn mice at 1, 3, 7, 10, and 14 days post-burn and from sham mice at 1 day post-burn. Plasma was obtained by centrifugation. Liquid chromatography-mass spectrometry was employed to identify plasma metabolites (hereinafter referred to as metabolites) and determine their temporal dynamics. Metabolites from sham mice at 1 day post-burn were compared with those from burn-injured mice at the same time point, and metabolites from burn-injured mice at 1, 3, 7, 10, and 14 days post-burn were analyzed. Additionally, the correlation between the selected metabolites and REE was analyzed, and the relevant metabolites underwent Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis.  Results  Compared with those in sham group, the daily and cumulative water intake and food intake of mice in burn group from days 1 to 14 post-burn showed no statistically significant differences overall (P>0.05); the body weight changes of mice in burn group were significantly reduced from days 2 to 14 post-burn (P<0.05); cumulative daytime and nighttime activity levels of mice in burn group were significantly reduced from days 1 to 14 post-burn (P<0.05); daily activity levels of mice in burn group lost normal circadian rhythms from days 1 to 14 post-burn; energy expenditure of mice in burn group was significantly reduced on day 1 post-burn (P<0.05) and significantly increased on days 7, 10, and 14 post-burn (P<0.05); the REE of mice in burn group was significantly increased from days 4 to 14 post-burn (P<0.05); respiratory entropy of mice in burn group was significantly reduced during nighttime from days 1 to 7 post-burn (P<0.05), while no significant difference was observed during daytime (P>0.05); carbohydrate oxidation rates of mice in burn group were significantly reduced at night on days 1 and 3 post-burn (P<0.05); fat oxidation rates of mice in burn group were significantly increased at night on days 1, 3, and 7 post-burn (P<0.05). A total of 450 metabolites were identified in plasma samples from mice. Among these, 253 metabolites exhibited specific temporal patterns and could be clustered into five distinct patterns. Eighty-five metabolites of burn-injured mice exhibited statistically significant changes following injury (P<0.05). Forty metabolites showed significant association with REE, with the top metabolites including linoleic acid, oleic acid, glucose, etc. KEGG pathway enrichment analysis revealed significant associations between REE and pathways including fatty acid synthesis and degradation, amino acid (e.g., isoleucine, tryptophan) synthesis and metabolism, and glycolysis/gluconeogenesis (P<0.05).  Conclusions  Both burn injury and time significantly influence energy metabolism patterns and material metabolism characteristics in mice. The hypermetabolic state persisted up to 14 days post-burn, with multiple fatty acid, amino acid, and glucose metabolic pathways associated with this elevated metabolism.

     

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