Advances in the research of application of three-dimensional bioprinting in skin tissue engineering

Deep skin wounds require skin grafts for coverage. Current treatments such as tissue engineered skin or skin substitutes can not meet the needs of clinical application due to the technical problems involving preservation, transportation, and a lengthy preparation process. In comparison with traditional methods such as freeze-drying, three-dimensional bioprinting can precisely dispense living cells, nucleic acid, growth factor, and phase-changing hydrogels according to the wound form, while maintaining high cell viability. Besides, it has excellent performance in high resolution, flexibility, reproducibility, and high throughput, showing great potential in the fabrication of tissue engineered skin. This review mainly introduces the common techniques of three-dimensional bioprinting, and their application in skin tissue engineering, focusing on the latest research progress, and summarizes the current challenges and future development of three-dimensional skin printing.