Experimental study on the effect of three-dimensional porous structures on the vascularization rate of artificial dermis

Tan Rongwei; Liu Xi; Chen Yingying; Xu Mengqiang; Guo Yuanjun; Wang Danyan; Liang Jiamei; Liu Jiao; Yuan Shasha; Fan Wei; Wang Xiangkun; She Zhending

doi:10.3760/cma.j.cn501120-20200715-00347

Volume 37 Issue 10

Oct. 2021

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Article Navigation > CHINESE JOURNAL OF BURNS AND WOUNDS > 2021 > 37(10): 959-969

Tan RW,Liu X,Chen YY,et al.Experimental study on the effect of three-dimensional porous structures on the vascularization rate of artificial dermis[J].Chin J Burns,2021,37(10):959-969.DOI: 10.3760/cma.j.cn501120-20200715-00347.

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Experimental study on the effect of three-dimensional porous structures on the vascularization rate of artificial dermis

doi: 10.3760/cma.j.cn501120-20200715-00347

1.
Shenzhen Lando Biomaterials Co., Ltd., Shenzhen Engineering Research Center of Implantable Medical Polymer, Guangdong Engineering Research Center of Implantable Medical Polymer, Shenzhen 518107, China
2.
Shenzhen Tsing Care Medical Co., Ltd., Shenzhen 518107, China
3.
Department of Burns, the Fifth Hospital of Baoding City, Baoding 071027, China

Funds:

National Key Research and Development Program Intergovernmental Key Projects for International Cooperation in Science, Technology and Innovation 2018YFE0194300

Special Project of High-end Medical Devices in Guangdong Province 2020B1111150001

Shenzhen Development and Reform Commission Strategic Emerging Industries Development Project 2019561

Shenzhen Technical Key Project JSGG20180504170419462

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Corresponding author: She Zhending, Email: shezd@landobiom.com
Received Date: 2020-07-15

Abstract

Abstract

Objective To explore the effects of orienting three-dimensional porous network (type A) and honeycomb briquette-shaped vertically penetrating three-dimensional porous network (type B) on the vascularization rate of artificial dermis. Methods The experimental research method was used. The artificial dermis was composed of a double layer of silicone layer and scaffold layer. Based on the difference of scaffold layer, they were divided into type A and type B artificial dermis (type A dermis and type B dermis, for short) containing type A and type B structure, respectively. The type A and type B structures were prepared by gradient freeze-drying technique and physical pore-making technique, respectively. The micro-morphology of two kinds of dermis scaffold was observed by scanning electron microscopy. The porosity of two kinds of dermis scaffold was measured by the Pyrex method. According to the method of national medical industry standard, the hydroxyproline content in degradation liquids and their residues in two kind of dermis were determined after degradation at 4, 8, 13, and 24 h, reflecting the degradation rates of two kinds of dermis. According to the random number table, L929 cells were divided into type A dermis group, type B dermis group, negative control group, and positive control group. The positive control group was added with minimum essential medium (MEM) containing 5% dimethyl sulfoxide, The negative control group was added with high-density polyethylene extract, and the other two groups were added with the corresponding extract. At 24 hours after culture, the growth rate of L929 cells was detected by methyl thiazolyl tetrazolium, and the cytotoxicity was graded. L929 cells and human umbilical vein endothelial cells (HUVECs) were inoculated into pore plates with two kinds of dermis preinstalled. On 1, 4, 7, and 14 d after inoculating, the adhesion and growth of L929 cells on the surfaces of the two kinds of scaffolds were detected by immunofluorescence method. On 7 d after inoculating, the migration of the above two kinds of cells into the two kinds of dermal scaffolds was detected by immunofluorescence and hematoxylin-eosin (HE) staining. Three full-thickness skin defect wounds of 5.0 cm×5.0 cm were created on both sides of the back of three 6-month-old healthy male Ba-Ma mini pigs. According to the random number table, six columns of wounds were divided into type A dermis two-step method group, type B dermis two-step method group, and type B dermis one-step method group. The wounds in type A dermis two-step method group and type B dermis two-step method group were transplanted with type A or type B dermis respectively before, and with autologous split-thickness skin grafting later. The wounds in type B dermis one-step method group were transplanted in a synchronous procedure including type B dermis (without silicone layer) and autologous skin grafting simultaneously. The bleeding, exudation, and infection of the wounds on the back in type A dermis two-step method group and type B dermis two-step method group on the 7th day after the second transplantation and in type B dermis one-step method group on the 14th day after the first transplantation were generally observed. The area of autologous skin graft was measured by the transparent film grid method, and the survival rate of autologous skin was calculated. On 4, 7, and 14 d after the first transplantation, the inflammatory cells, fibroblasts (Fbs), and capillary infiltration into the scaffolds of the three groups were detected by HE staining. On 7, 14 d after the first transplantation, the vascularization of the scaffolds was further observed by immunohistochemistry. On 28, 90 d after the first operation, the degradation of the scaffolds of type A dermis and type B dermis was observed by HE staining. Data were statistically analyzed with one-way analysis of variance, independent sample t test, and Bonferroni correction. Results A large number of round and oval micropores were evenly distributed on the surface of type A scaffold, and the cylindrical hole walls could be observed arranging in a parallel direction in the longitudinal section. The honeycomb briquette-shaped penetrating macropores on the surface of type B scaffold were arranged in an orderly matrix. The pore walls of the honeycomb briquette-shaped penetrating macropores were connected by micropores to form a network structure. The porosity of type A dermis was (93.21±0.72)%, which was similar to (95.88±1.00)% of type B dermis (t=4.653, P>0.05). The degradation rates of type A dermis at 4, 8, 13, and 24 h were similar to those of type B dermis at the corresponding time point (t=0.232, 0.856, 0.258, 7.716, P>0.05). At 24 h after culture, the proliferation rates of L929 cells in the type A dermis group, type B dermis group, and negative control group were significantly higher than those of the positive control group (t=2 393.46, 2 538.27, 1 077.77, P<0.01). The cytotoxicity rating of cells in positive control group was grade 4, while that of the other three groups was grade zero. On 1, 4, 7, and 14 d after inoculation, both L929 cells and HUVECs proliferated in a time-dependent manner in two kinds of dermal scaffolds. The adhesion growth and proliferation rate of the two kinds of cells on the surface of type B dermis was higher than that of type A dermis. On 7 d after inoculation, both L929 cells and HUVECs covered the surface of type B dermis and migrated into one side of the silicone layer. However, the above two kinds of cells migrated slowly into type A dermis, and only a few cells were found on one side of the silicone layer. There was no bleeding, exudation, or infection in the wounds repaired by type A and type B dermis. The survival rate of autologous skin grafting of 6 wounds in each group was 100%. On 4, 7, and 14 d after the first operation, inflammatory cells, Fbs, and capillaries gradually infiltrated into the scaffold layer, and the cell infiltration rate from high to low was type B dermis one-step method group, type B dermis two-step method group, and type A dermis two-step method group. The scaffold in wound in the type B dermis one-step method group gradually collapsed on 28 d after the first operation, and completely degraded in 3 months after the first operation. The scaffold degradation rate of type A dermis two-step method group was similar to that mentioned above. Conclusions The honeycomb briquette-shaped vertically penetrating three-dimensional porous network structure of type B scaffold can accelerate its vascularization process, which is beneficial to autogenous split-thickness skin in one-step procedure to repair full-thickness skin defects wound in Ba-Ma mini pigs. Compared with the "two-step method" of staged transplantation of type A scaffold and autologous split-thickness skin, and one-step transplantation has equal efficacy and can provide a better choice for wound treatment.
- Skin, artificial,
- Wound healing,
- Three-dimensional,
- Pore structure,
- Full-thickness skin defect,
- Vascularization

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References

[1]	VarkeyM, VisscherDO, van ZuijlenPPM, et al. Skin bioprinting: the future of burn wound reconstruction?[J/OL]. Burns Trauma,2019,7:4[2021-05-06].https://pubmed.ncbi.nlm.nih.gov/30805375/.DOI: 10.1186/s41038-019-0142-7.
[2]	李士,宋永焕,周飞亚,等.人工真皮复合全厚皮修复儿童足踝部皮肤软组织缺损[J].中华小儿外科杂志,2014,35(1):36-38.DOI: 10.3760/cma.j.issn.0253-3006.2014.01.009.
[3]	HeimbachD,LutermanA,BurkeJ,et al.Artificial dermis for major burns. A multi-center randomized clinical trial[J].Ann Surg,1988,208(3):313-320.DOI: 10.1097/00000658-198809000-00008.
[4]	YannasIV,TzeranisDS,SoP.Regeneration mechanism for skin and peripheral nerves clarified at the organ and molecular scales[J].Curr Opin Biomed Eng,2018,6:1-7.DOI: 10.1016/j.cobme.2017.12.002.
[5]	WangW, ZhangL, SunL, et al. Biocompatibility and immunotoxicology of the preclinical implantation of a collagen-based artificial dermal regeneration matrix[J].Biomed Environ Sci,2018,31(11):829-842.DOI: 10.3967/bes2018.110.
[6]	弓辰,唐洪泰,王光毅,等.国产人工真皮移植结合自体皮移植修复骨质肌腱外露创面的疗效评价[J/CD].中华损伤与修复杂志:电子版,2016,11(1):34-39.DOI: 10.3877/cma.j.issn.1673-9450.2016.01.008.
[7]	王丽英,黄红军,牛希华.人工真皮联合自体薄皮片移植和自体中厚皮移植治疗烧伤后增生性瘢痕的疗效比较[J].中国医疗美容,2018,8(2):26-29.DOI: 10.19593/j.issn.2095-0721.2018.02.009.
[8]	《双层人工真皮临床应用专家共识(2019版)》编写组.双层人工真皮临床应用专家共识(2019版)[J].中华烧伤杂志,2019,35(10):705-711.DOI: 10.3760/cma.j.issn.1009-2587.2019.10.001.
[9]	FruehFS,MengerMD,LindenblattN,et al.Current and emerging vascularization strategies in skin tissue engineering[J].Crit Rev Biotechnol,2017,37(5):613-625.DOI: 10.1080/07388551.2016.1209157.
[10]	石桂欣, 王身国, 贝建中. 聚乳酸与聚乳酸—羟基乙酸多孔细胞支架的制备及孔隙的表征[J]. 功能高分子学报, 2001, 14(1): 7-11.DOI: 10.14133/j.cnki.1008-9357.2001.01.014.
[11]	BoyceST,HansbroughJF.Biologic attachment, growth, and differentiation of cultured human epidermal keratinocytes on a graftable collagen and chondroitin-6-sulfate substrate[J].Surgery,1988,103(4):421-431.
[12]	陈欣,副岛一孝,野崎斡弘,等.成纤维细胞移植促进人工真皮内血管新生的研究[J]. 中国修复重建外科杂志, 2004, 18(3): 205-208.
[13]	UtzingerU,BaggettB,WeissJA,et al.Large-scale time series microscopy of neovessel growth during angiogenesis[J].Angiogenesis,2015,18(3):219-232.DOI: 10.1007/s10456-015-9461-x.
[14]	AgostiniT,DiniM,Quattrini LiA,et al.A novel combined surgical approach to head and neck dermatofibrosarcoma protuberans[J].J Craniomaxillofac Surg,2013,41(7):681-685.DOI: 10.1016/j.jcms.2013.01.009.
[15]	JeschkeMG,RoseC,AngeleP,et al.Development of new reconstructive techniques: use of Integra in combination with fibrin glue and negative-pressure therapy for reconstruction of acute and chronic wounds[J].Plast Reconstr Surg,2004,113(2):525-530.DOI: 10.1097/01.PRS.0000100813.39746.5A.
[16]	LvZ,YuL,WangQ,et al.Dermal regeneration template and vacuum sealing drainage for treatment of traumatic degloving injuries of upper extremity in a single-stage procedure[J].ANZ J Surg,2019,89(7/8):950-954.DOI: 10.1111/ans.15315.
[17]	WainwrightD,MaddenM,LutermanA,et al.Clinical evaluation of an acellular allograft dermal matrix in full-thickness burns[J].J Burn Care Rehabil,1996,17(2):124-136.DOI: 10.1097/00004630-199603000-00006.
[18]	郑东风,谭谦,吴杰,等.脱细胞异体真皮加自体刃厚皮片修复皮瓣供区创面[J].中国美容医学,2012,21(16):3-4.DOI: 10.3969/j.issn.1008-6455.2012.16.002.
[19]	MüllerCS,SchiekoferC,KörnerR,et al.Improved patient-centered care with effective use of Integra® in dermatologic reconstructive surgery[J].J Dtsch Dermatol Ges,2013,11(6):537-548.DOI: 10.1111/ddg.12038.
[20]	HurGY,SeoDK,LeeJW.Contracture of skin graft in human burns: effect of artificial dermis[J].Burns,2014,40(8):1497-1503.DOI: 10.1016/j.burns.2014.08.007.
[21]	WollinaU.One-stage reconstruction of soft tissue defects with the sandwich technique: collagen-elastin dermal template and skin grafts[J].J Cutan Aesthet Surg,2011,4(3):176-182.DOI: 10.4103/0974-2077.91248.
[22]	SoejimaK, ShimodaK, KashimuraT, et al. One-step grafting procedure using artificial dermis and split-thickness skin in burn patients[J]. European Journal of Plastic Surgery, 2013, 36(9): 585-590. DOI: 10.1007/s00238-012-0785-0.
[23]	梁黎明,柴家科,杨红明,等.激光微孔猪脱细胞真皮基质制备及创面移植的观察[J].中华烧伤杂志,2007,23(2):122-125.DOI: 10.3760/cma.j.issn.1009-2587.2007.02.012.
[24]	罗旭,万丽,李安乐,等.新型激光微孔化猪脱细胞真皮基质的制备及应用[J/CD].中华损伤与修复杂志:电子版,2012,7(3):240-244.DOI: 10.3877/cma.j.issn.1673-9450.2012.03.005.
[25]	盛小辉,朱宝昌,冯世海,等.激光微孔脱细胞猪真皮支架对Ⅲ度皮肤烧伤血管化的影响[J].天津医科大学学报,2016,22(1):17-20.
[26]	BaiF,WangZ,LuJ,et al.The correlation between the internal structure and vascularization of controllable porous bioceramic materials in vivo: a quantitative study[J].Tissue Eng Part A,2010,16(12):3791-3803.DOI: 10.1089/ten.TEA.2010.0148.
[27]	ChoiSW,ZhangY,MacewanMR,et al.Neovascularization in biodegradable inverse opal scaffolds with uniform and precisely controlled pore sizes[J].Adv Healthc Mater,2013,2(1):145-154.DOI: 10.1002/adhm.201200106.
[28]	LaschkeMW,HarderY,AmonM,et al.Angiogenesis in tissue engineering: breathing life into constructed tissue substitutes[J].Tissue Eng,2006,12(8):2093-2104.DOI: 10.1089/ten.2006.12.2093.
[29]	ValarmathiMT,DavisJM,YostMJ,et al.A three-dimensional model of vasculogenesis[J].Biomaterials,2009,30(6):1098-1112.DOI: 10.1016/j.biomaterials.2008.10.044.
[30]	QiuX,WangJ,WangG,et al.Vascularization of Lando® dermal scaffold in an acute full-thickness skin-defect porcine model[J].J Plast Surg Hand Surg,2018,52(4):204-209.DOI: 10.1080/2000656X.2017.1421547.
[31]	KoenenW,FelchtM,VockenrothK,et al.One-stage reconstruction of deep facial defects with a single layer dermal regeneration template[J].J Eur Acad Dermatol Venereol,2011,25(7):788-793.DOI: 10.1111/j.1468-3083.2010.03863.x.
[32]	Böttcher-HaberzethS,BiedermannT,SchiestlC,et al.Matriderm® 1 mm versus Integra® Single Layer 1.3 mm for one-step closure of full thickness skin defects: a comparative experimental study in rats[J].Pediatr Surg Int,2012,28(2):171-177.DOI: 10.1007/s00383-011-2990-5.

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Experimental study on the effect of three-dimensional porous structures on the vascularization rate of artificial dermis

doi: 10.3760/cma.j.cn501120-20200715-00347