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Article Navigation >  CHINESE JOURNAL OF BURNS AND WOUNDS  >  > Accepted Manuscript
Guan Hao,Zhang Hao,Zhang Wanfu,et al.Establishment and efficacy evaluation of a rabbit lower limb partial-thickness scald model[J].Chin J Burns Wounds,2026,42(2):1-9.DOI: 10.3760/cma.j.cn501225-20241028-00418.
Citation: Guan Hao,Zhang Hao,Zhang Wanfu,et al.Establishment and efficacy evaluation of a rabbit lower limb partial-thickness scald model[J].Chin J Burns Wounds,2026,42(2):1-9.DOI: 10.3760/cma.j.cn501225-20241028-00418.
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Establishment and efficacy evaluation of a rabbit lower limb partial-thickness scald model

doi: 10.3760/cma.j.cn501225-20241028-00418

  • Department of Burns and Cutaneous Surgery, Burn Center of PLA, the First Affiliated Hospital of Air Force Medical University, Xi'an 710032, China

Funds:

National Natural Science Foundation of China (General Program) 82272268

Key Research and Development Program of Shaanxi Province 2024SF-ZDCYL-04-10

More Information
  • Corresponding author: Guan Hao, Email: guanhao2020@yeah.net
  • Received Date: 2024-10-28
    Available Online: 2026-01-30
    Abstract
  •   Objective  To establish a rabbit lower limb partial-thickness scald model and evaluate its efficacy.  Methods  This study was a repeated measures designed and group designed experimental study. Forty healthy male New Zealand rabbits aged 6 to 8 months were selected, and divided into scald group (30 rabbits) and normal control group (10 rabbits) according to the random number table method. The rabbits in scald group were anesthetized, then a bucket-type constant temperature water bath pot and a self-made rabbit scald fixation bracket were used to immerse the right lower limb in water at 70 ℃ for 12 s to prepare a rabbit lower limb partial-thickness scald model, then fluid supplementation were conducted within 6 h after injury. The rabbits in normal control group were subjected to hair removal and anesthesia as scald group at the same time point except for scald and fluid supplementation. Ten rabbits were randomly selected from scald group, and the wound tissue morphology was observed after hematoxylin-eosin staining before injury and at 6 and 24 h after injury, respectively. Another 10 rabbits in scald group were observed for skin blood perfusion in right lower limb using laser Doppler flowmetry before injury and at 0 (immediate), 1, 3, 6, 12, 24, and 48 h after injury, respectively. The remaining 10 rabbits in scald group were measured for subcutaneous and deep muscle temperatures in right lower limb using non-contact infrared temperature sensors and contact temperature sensors before injury and at 10 s, 20 s, 40 s, 2 min, 10 min, 20 min, 40 min, 1 h, 2 h, 3 h, 4 h, 5 h, and 6 h after injury, respectively. After blood perfusion observation, the 10 rabbits in scald group were measured for right lower limb circumference before injury and at 1, 2, 3, 4, 5, 6, 24, 48, and 72 h after injury, respectively, and wound healing time of dorsal foot, plantar foot, and crural regions were recorded. Ten rabbits from normal control group and 10 rabbits after temperature measurement from scald group were taken to detect the serum levels of interleukin-1β (IL-1β), IL-6, tumor necrosis factor-α (TNF-α), superoxide dismutase (SOD), and malondialdehyde by enzyme-linked immunosorbent assay.  Results  Before injury, epidermis and skin appendages of the right lower limb of rabbits in scald group were intact. At 6 h after injury, epidermal continuity was interrupted, partial skin appendages disappeared, interstitial spaces increased, and edema increased. At 24 h after injury, skin appendages atrophied and disappeared, tissue edema increased significantly, and inflammatory cell infiltration increased in superficial dermis. The skin blood perfusion of right lower limb of rabbits in scald group before injury and at 0, 1, 3, 6, 12, 24, and 48 h after injury was (1.00±0.26), (1.03±0.29), (1.04±0.29), (1.19±0.37), (1.30±0.50), (1.36±0.99), (1.39±0.22), and (0.72±0.21) perfusion units, respectively (main effect of time factor, F=12.55, P<0.05). The subcutaneous temperature in right lower limb of rabbits in scald group before injury and at 10 s, 20 s, 2 min, 10 min, 20 min, 40 min, 1 h, 2 h, 3 h, 4 h, 5 h, 6 h after injury was significantly lower than that at 40 s after injury (P<0.05). The deep muscle temperature in right lower limb of rabbits in scald group before injury and at 10 s, 20 s, 40 s, 2 min, 20 min, 40 min, 1 h, 2 h, 3 h, 4 h, 5 h, and 6 h after injury was significantly lower than that at 10 min after injury (P<0.05). The right lower limb circumference in rabbits in scald group at 24 h after injury was significantly larger than that before injury and at 1, 2, 3, 4, 5, 6, 48, and 72 h after injury (with P values all <0.05). The wound healing time of plantar foot of rabbits in scald group was significantly shorter than that of dorsal foot and crural region (with P values both <0.05). At 24 h after injury, compared with those in normal control group, serum levels of IL-1β, IL-6, TNF-α, and malondialdehyde in rabbits in scald group were significantly increased (with t values of 21.92, 7.48, 12.58, and 117.34, respectively, P<0.05), while serum level of SOD was significantly decreased (t=117.34, P<0.05).  Conclusions  The rabbit lower limb partial-thickness scald model established in this study can safely and stably simulate the pathophysiological process and clinical characteristics of human limb scald, thus is a favorable model for studying partial-thickness scald of human limbs.

     

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