Chen W,Chang SS,Zhou J,et al.Clinical effects of antibiotic bone cement combined with free anterolateral thigh flap in sequential treatment of diabetic foot ulcer[J].Chin J Burns Wounds,2023,39(4):319-324.DOI: 10.3760/cma.j.cn501225-20220628-00267.
Citation: Bai XZ,Tao K,Liu Y,et al.Effects and underlying mechanism of human adipose mesenchymal stem cells-derived exosomes on acute lung injury in septic mice[J].Chin J Burns Wounds,2024,40(12):1132-1142.DOI: 10.3760/cma.j.cn501225-20240927-00355.

Effects and underlying mechanism of human adipose mesenchymal stem cells-derived exosomes on acute lung injury in septic mice

doi: 10.3760/cma.j.cn501225-20240927-00355
Funds:

General Program of National Natural Science Foundation of China 82272269

More Information
  •   Objective  To explore the effects and underlying mechanism of human adipose mesenchymal stem cells (ADSC)-derived exosomes on acute lung injury in septic mice.  Methods  The study was an experimental study. Human ADSC of passages 4-5 were selected, and exosomes in their supernatant were isolated and extracted by differential ultracentrifugation. Exosomes were then used after identification. Twenty-four adult male BALB/c mice were selected and divided into normal control group, simple cecal ligation and puncture (CLP) group, and CLP+ADSC-exosome group according to the random number table method (the grouping method was the same below), with 8 mice in each group. The mice in simple CLP group were injected with phosphate buffer after CLP surgery (to establish an animal model of acute lung injury in septic mice), the mice in CLP+ADSC-exosome group were treated according to the corresponding group name, and the mice in normal control group were only injected with phosphate buffer. At 24 hours after surgery, the morphology of lung tissue was observed by hematoxylin-eosin staining, the apoptosis of lung tissue cells was detected by in-situ end-labeling method, the content of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in the serum of mice was detected by enzyme-linked immunosorbent assay, the content of malondialdehyde and superoxide dismutase (SOD) in lung tissue was detected by microplate reader, and the expressions of CD86 and CD206 in mouse lung tissue cells was detected by immunofluorescence method. Mouse macrophage RAW264.7 was taken and divided into blank control group, simple lipopolysaccharide (LPS) group, and LPS+ADSC-exosome group. The cells of LPS+ADSC-exosome group and simple LPS group were cultured by adding LPS+ADSC-exosome and LPS, respectively, and cells in blank control group were routinely cultured. Twelve hours after culture, the ATP content, the percentage of mitochondrial reactive oxygen species positive cells, as well as mitochondrial membrane potential in cells were detected by related detection kits. The mRNA expression levels of M1 polarization marker inducible nitric oxide synthase (iNOS), M2 polarization marker arginase-1 (Arg1), and inflammatory factors TNF-α and IL-1β in cells were detected by real-time fluorescence quantitative reverse-transcription polymerase chain reaction method. Three samples were used for mRNA expression detection, and four samples were used for the detection of the other indicators.  Results  At 24 hours after surgery, the structure of mouse lung tissues in normal control group was clear and intact without inflammatory cell infiltration. Compared with that in normal control group, the lung tissue edema as well as the infiltration of inflammatory cells of mice was much more obvious in simple CLP group. However, compared with that in simple CLP group, the lung tissue edema of mice in CLP+ADSC-exosome group was significantly alleviated, the infiltration of inflammatory cells was significantly reduced, and the cell apoptosis and necrosis were significantly improved. Twenty-four hours after surgery, compared with that in normal control group, the levels of TNF-α and IL-1β in the serum of mice in simple CLP group were significantly increased (with t values of 50.82 and 30.81, respectively, P<0.05); compared with that in simple CLP group, the levels of TNF-α and IL-1β in the serum of mice in CLP+ADSC-exosome group were significantly decreased (with t values of 16.36 and 19.25, respectively, P<0.05). Compared with that in normal control group, the content of malondialdehyde in the lung tissue of mice in simple CLP group was significantly increased (t=9.89, P<0.05); and the content of SOD was significantly decreased (t=5.01, P<0.05); compared with that in simple CLP group, the content of malondialdehyde in the lung tissue of mice in CLP+ADSC-exosome group was significantly decreased (t=4.38, P<0.05), and the content of SOD was significantly increased (t=2.97, P<0.05). Twenty-four hours after surgery, compared with that in normal control group, the proportion of CD86 positive cells in the lung tissue of mice in simple CLP group was significantly increased, and the proportion of CD206 positive cells was significantly decreased; compared with that in simple CLP group, the proportion of CD86 positive cells in the lung tissue of mice in CLP+ADSC-exosome group was significantly decreased, and the proportion of CD206 positive cells was significantly increased. After 12 hours of culture, compared with that in blank control group, the ATP content of RAW264.7 cells in simple LPS group was significantly decreased (t=6.28, P<0.05); compared with that in simple LPS group, the ATP content of RAW264.7 cells in LPS+ADSC-exosome group was significantly increased (t=4.01, P<0.05). After 12 hours of culture, compared with (22±4)% in blank control group, (40±6)% of positive cells of mitochondrial reactive oxygen species in RAW264.7 cells in simple LPS group was significantly increased (t=5.04, P<0.05); compared with that in LPS group, (30±5)% of positive cells of mitochondrial reactive oxygen species in RAW264.7 cells in LPS+ADSC-exosome group was significantly decreased (t=2.65, P<0.05). After 12 hours of culture, compared with that in blank control group, the mitochondrial membrane potential of RAW264.7 cells in simple LPS group was significantly decreased; the mitochondrial membrane potential of RAW264.7 cells in LPS+ ADSC-exosome group was between those in blank control group and simple LPS group. After 12 hours of culture, compared with that in blank control group, the mRNA expressions of TNF-α, IL-1β, and iNOS in RAW264.7 cells in simple LPS group were significantly increased (with t values of 16.51, 31.04, and 7.70, respectively, P<0.05), and the decrease in the mRNA expression of Arg1 was not statistically significant (P>0.05); compared with that in simple LPS group, the mRNA expressions of TNF-α, IL-1β, and iNOS in RAW264.7 cells in LPS+ADSC-exosome group were significantly decreased (with t values of 11.38, 22.58, and 5.28, respectively, P<0.05), and the mRNA expression of Arg1 was significantly increased (t=7.66, P<0.05).  Conclusions  Human ADSC-exosomes may play a role in improving lung injury in septic mice by improving LPS-induced mitochondrial dysfunction in mice macrophages, inhibiting the polarization of macrophages toward M1, and reducing the inflammatory response.

     

  • [1]
    LongME, MallampalliRK, HorowitzJC. Pathogenesis of pneumonia and acute lung injury[J]. Clin Sci (Lond), 2022,136(10):747-769. DOI: 10.1042/CS20210879.
    [2]
    RawalG, YadavS, KumarR. Acute respiratory distress syndrome: an update and review[J]. J Transl Int Med, 2018,6(2):74-77. DOI: 10.1515/jtim-2016-0012.
    [3]
    JohnsonER, MatthayMA. Acute lung injury: epidemiology, pathogenesis, and treatment[J]. J Aerosol Med Pulm Drug Deliv, 2010,23(4):243-252. DOI: 10.1089/jamp.2009.0775.
    [4]
    LuhSP, ChiangCH. Acute lung injury/acute respiratory distress syndrome (ALI/ARDS): the mechanism, present strategies and future perspectives of therapies[J]. J Zhejiang Univ Sci B, 2007,8(1):60-69. DOI: 10.1631/jzus.2007.B0060.
    [5]
    LocatiM, CurtaleG, DiversityMantovani A., mechanisms, and significance of macrophage plasticity[J]. Annu Rev Pathol, 2020,15:123-147. DOI: 10.1146/annurev-pathmechdis-012418-012718.
    [6]
    OishiY, ManabeI. Macrophages in inflammation, repair and regeneration[J]. Int Immunol, 2018,30(11):511-528. DOI: 10.1093/intimm/dxy054.
    [7]
    GibbingsSL, ThomasSM, AtifSM, et al. Three unique interstitial macrophages in the murine lung at steady state[J]. Am J Respir Cell Mol Biol, 2017,57(1):66-76. DOI: 10.1165/rcmb.2016-0361OC.
    [8]
    AggarwalNR, KingLS, D'AlessioFR. Diverse macrophage populations mediate acute lung inflammation and resolution[J]. Am J Physiol Lung Cell Mol Physiol, 2014,306(8):L709-725. DOI: 10.1152/ajplung.00341.2013.
    [9]
    SchumackerPT, GillespieMN, NakahiraK, et al. Mitochondria in lung biology and pathology: more than just a powerhouse[J]. Am J Physiol Lung Cell Mol Physiol, 2014,306(11):L962-974. DOI: 10.1152/ajplung.00073.2014.
    [10]
    KellnerM, NoonepalleS, LuQ, et al. ROS signaling in the pathogenesis of acute lung injury (ALI) and acute respiratory distress syndrome (ARDS)[J]. Adv Exp Med Biol, 2017,967:105-137. DOI: 10.1007/978-3-319-63245-2_8.
    [11]
    TanHY, WangN, LiS, et al. The reactive oxygen species in macrophage polarization: reflecting its dual role in progression and treatment of human diseases[J]. Oxid Med Cell Longev, 2016,2016:2795090. DOI: 10.1155/2016/2795090.
    [12]
    Van den BosscheJ, BaardmanJ, OttoNA, et al. Mitochondrial dysfunction prevents repolarization of inflammatory macrophages[J]. Cell Rep, 2016,17(3):684-696. DOI: 10.1016/j.celrep.2016.09.008.
    [13]
    邱煜程, 周显玉, 刘菲, 等. 间充质干细胞及其外泌体在移植中的应用进展[J].组织工程与重建外科杂志,2023,19(2):184-188. DOI: 10.3969/j.issn.1673-0364.2023.02.016.
    [14]
    YuT, LiuH, GaoM, et al. Dexmedetomidine regulates exosomal miR-29b-3p from macrophages and alleviates septic myocardial injury by promoting autophagy in cardiomyocytes via targeting glycogen synthase kinase3β[J/OL]. Burns Trauma, 2024,12:tkae042[2024-09-27].https://pubmed.ncbi.nlm.nih.gov/39502342/. DOI: 10.1093/burnst/tkae042.
    [15]
    蒲倩, 修光辉, 孙洁, 等. 间充质干细胞外泌体在脓毒症多器官功能障碍中作用的研究进展[J].中华危重病急救医学,2021,33(6):757-760. DOI: 10.3760/cma.j.cn121430-20200908-00620.
    [16]
    HuQ, LyonCJ, FletcherJK, et al. Extracellular vesicle activities regulating macrophage- and tissue-mediated injury and repair responses[J]. Acta Pharm Sin B, 2021,11(6):1493-1512. DOI: 10.1016/j.apsb.2020.12.014.
    [17]
    JingW, WangH, ZhanL, et al. Extracellular vesicles, new players in sepsis and acute respiratory distress syndrome[J]. Front Cell Infect Microbiol, 2022,12:853840. DOI: 10.3389/fcimb.2022.853840.
    [18]
    HommaK, BazhanovN, HashimotoK, et al. Mesenchymal stem cell-derived exosomes for treatment of sepsis[J]. Front Immunol, 2023,14:1136964. DOI: 10.3389/fimmu.2023.1136964.
    [19]
    GongT, LiuYT, FanJ. Exosomal mediators in sepsis and inflammatory organ injury: unraveling the role of exosomes in intercellular crosstalk and organ dysfunction[J]. Mil Med Res, 2024,11(1):24. DOI: 10.1186/s40779-024-00527-6.
    [20]
    BaiX, LiJ, LiL, et al. Extracellular vesicles from adipose tissue-derived stem cells affect Notch-miR148a-3p axis to regulate polarization of macrophages and alleviate sepsis in mice[J]. Front Immunol, 2020,11:1391. DOI: 10.3389/fimmu.2020.01391.
    [21]
    DejagerL, PinheiroI, DejonckheereE, et al. Cecal ligation and puncture: the gold standard model for polymicrobial sepsis?[J]. Trends Microbiol, 2011,19(4):198-208. DOI: 10.1016/j.tim.2011.01.001.
    [22]
    JiaoY, ZhangT, ZhangC, et al. Exosomal miR-30d-5p of neutrophils induces M1 macrophage polarization and primes macrophage pyroptosis in sepsis-related acute lung injury[J]. Crit Care, 2021,25(1):356. DOI: 10.1186/s13054-021-03775-3.
    [23]
    BaiX, HeT, LiuY, et al. Acetylation-dependent regulation of notch signaling in macrophages by SIRT1 affects sepsis development[J]. Front Immunol, 2018,9:762. DOI: 10.3389/fimmu.2018.00762.
    [24]
    蔡维霞, 沈括, 曹涛, 等. 人脂肪间充质干细胞来源外泌体对脓毒症小鼠肺血管内皮细胞损伤的影响及其机制[J].中华烧伤与创面修复杂志,2022,38(3):266-275. DOI: 10.3760/cma.j.cn501120-20211020-00362.
    [25]
    ShenK, WangX, WangY, et al. miR-125b-5p in adipose derived stem cells exosome alleviates pulmonary microvascular endothelial cells ferroptosis via Keap1/Nrf2/GPX4 in sepsis lung injury[J]. Redox Biol, 2023,62:102655. DOI: 10.1016/j.redox.2023.102655.
    [26]
    WuH, WangY, ZhangY, et al. Breaking the vicious loop between inflammation, oxidative stress and coagulation, a novel anti-thrombus insight of nattokinase by inhibiting LPS-induced inflammation and oxidative stress[J]. Redox Biol, 2020,32:101500. DOI: 10.1016/j.redox.2020.101500.
    [27]
    XuH, QiQ, YanX. Myricetin ameliorates sepsis-associated acute lung injury in a murine sepsis model[J]. Naunyn Schmiedebergs Arch Pharmacol, 2021,394(1):165-175. DOI: 10.1007/s00210-020-01880-8.
    [28]
    JinC, ChenJ, GuJ, et al. Gut-lymph-lung pathway mediates sepsis-induced acute lung injury[J]. Chin Med J (Engl), 2020,133(18):2212-2218. DOI: 10.1097/CM9.0000000000000928.
    [29]
    YehyaN, SmithL, ThomasNJ, et al. Definition, incidence, and epidemiology of pediatric acute respiratory distress syndrome: from the second pediatric acute lung injury consensus conference[J]. Pediatr Crit Care Med, 2023,24(12 Suppl 2):S87-98. DOI: 10.1097/PCC.0000000000003161.
    [30]
    WangS, HuL, FuY, et al. Inhibition of IRE1α/XBP1 axis alleviates LPS-induced acute lung injury by suppressing TXNIP/NLRP3 inflammasome activation and ERK/p65 signaling pathway[J]. Respir Res, 2024,25(1):417. DOI: 10.1186/s12931-024-03044-1.
    [31]
    ButtY, KurdowskaA, AllenTC. Acute lung injury: a clinical and molecular review[J]. Arch Pathol Lab Med, 2016,140(4):345-350. DOI: 10.5858/arpa.2015-0519-RA.
    [32]
    赵松韵, 万志杰, 曹曦元, 等. 靶向DNA损伤应答在小细胞肺癌中的作用研究进展[J].解放军医学杂志,2022,47(8):838-844. DOI: 10.11855/j.issn.0577-7402.2022.08.0838.
    [33]
    李林, 邢福席, 付全有, 等. 脓毒症急性肺损伤治疗的研究进展[J].中华医院感染学杂志,2024,34(1):149-155. DOI: 10.11816/cn.ni.2024-236123.
    [34]
    ZhangW, ChenH, XuZ, et al. Liensinine pretreatment reduces inflammation, oxidative stress, apoptosis, and autophagy to alleviate sepsis acute kidney injury[J]. Int Immunopharmacol, 2023,122:110563. DOI: 10.1016/j.intimp.2023.110563.
    [35]
    Bar-OrD, CarrickMM, MainsCW, et al. Sepsis, oxidative stress, and hypoxia: are there clues to better treatment?[J] Redox Rep, 2015,20(5):193-197. DOI: 10.1179/1351000215Y.0000000005.
    [36]
    JoffreJ, HellmanJ. Oxidative stress and endothelial dysfunction in sepsis and acute inflammation[J]. Antioxid Redox Signal, 2021,35(15):1291-1307. DOI: 10.1089/ars.2021.0027.
    [37]
    ChenX, TangJ, ShuaiW, et al. Macrophage polarization and its role in the pathogenesis of acute lung injury/acute respiratory distress syndrome[J]. Inflamm Res, 2020,69(9):883-895. DOI: 10.1007/s00011-020-01378-2.
    [38]
    YuanY, FanG, LiuY, et al. Correction to: the transcription factor KLF14 regulates macrophage glycolysis and immune function by inhibiting HK2 in sepsis[J]. Cell Mol Immunol, 2022,19(5):650. DOI: 10.1038/s41423-022-00839-4.
    [39]
    WangX, ChenS, LuR, et al. Adipose-derived stem cell-secreted exosomes enhance angiogenesis by promoting macrophage M2 polarization in type 2 diabetic mice with limb ischemia via the JAK/STAT6 pathway[J]. Heliyon, 2022,8(11):e11495. DOI: 10.1016/j.heliyon.2022.e11495.
    [40]
    ZhaoH, ShangQ, PanZ, et al. Exosomes from adipose-derived stem cells attenuate adipose inflammation and obesity through polarizing M2 macrophages and beiging in white adipose tissue[J]. Diabetes, 2018,67(2):235-247. DOI: 10.2337/db17-0356.
    [41]
    WestAP, BrodskyIE, RahnerC, et al. TLR signalling augments macrophage bactericidal activity through mitochondrial ROS[J]. Nature, 2011,472(7344):476-480. DOI: 10.1038/nature09973.
    [42]
    WestAP, Khoury-HanoldW, StaronM, et al. Mitochondrial DNA stress primes the antiviral innate immune response[J]. Nature, 2015,520(7548):553-557. DOI: 10.1038/nature14156.
    [43]
    HongP, YangH, WuY, et al. The functions and clinical application potential of exosomes derived from adipose mesenchymal stem cells: a comprehensive review[J]. Stem Cell Res Ther, 2019,10(1):242. DOI: 10.1186/s13287-019-1358-y.
    [44]
    WangZ, WhiteA, WangX, et al. Mitochondrial fission mediated cigarette smoke-induced pulmonary endothelial injury[J]. Am J Respir Cell Mol Biol, 2020,63(5):637-651. DOI: 10.1165/rcmb.2020-0008OC.
    [45]
    VidelaLA, MarimánA, RamosB, et al. Standpoints in mitochondrial dysfunction: underlying mechanisms in search of therapeutic strategies[J]. Mitochondrion, 2022,63:9-22. DOI: 10.1016/j.mito.2021.12.006.
    [46]
    HoffmannRF, ZarrintanS, BrandenburgSM, et al. Prolonged cigarette smoke exposure alters mitochondrial structure and function in airway epithelial cells[J]. Respir Res, 2013,14(1):97. DOI: 10.1186/1465-9921-14-97.
    [47]
    GalleyHF. Oxidative stress and mitochondrial dysfunction in sepsis[J]. Br J Anaesth, 2011, 107(1):57-64. DOI: 10.1093/bja/aer093.
    [48]
    BhattiJS, BhattiGK, ReddyPH. Mitochondrial dysfunction and oxidative stress in metabolic disorders-a step towards mitochondria based therapeutic strategies[J]. Biochim Biophys Acta Mol Basis Dis, 2017,1863(5):1066-1077. DOI: 10.1016/j.bbadis.2016.11.010.
    [49]
    XianH, LiuY, Rundberg NilssonA, et al. Metformin inhibition of mitochondrial ATP and DNA synthesis abrogates NLRP3 inflammasome activation and pulmonary inflammation[J]. Immunity, 2021,54(7):1463-1477.e11. DOI: 10.1016/j.immuni.2021.05.004.
    [50]
    ZhongZ, LiangS, Sanchez-LopezE, et al. New mitochondrial DNA synthesis enables NLRP3 inflammasome activation[J]. Nature, 2018,560(7717):198-203. DOI: 10.1038/s41586-018-0372-z.
    [51]
    XuZ, ShenJ, LinL, et al. Exposure to irregular microplastic shed from baby bottles activates the ROS/NLRP3/Caspase-1 signaling pathway, causing intestinal inflammation[J]. Environ Int, 2023,181:108296. DOI: 10.1016/j.envint.2023.108296.
    [52]
    ChenY, YeX, EscamesG, et al. The NLRP3 inflammasome: contributions to inflammation-related diseases[J]. Cell Mol Biol Lett, 2023,28(1):51. DOI: 10.1186/s11658-023-00462-9.
    [53]
    MittalM, SiddiquiMR, TranK, et al. Reactive oxygen species in inflammation and tissue injury[J]. Antioxid Redox Signal, 2014,20(7):1126-1167. DOI: 10.1089/ars.2012.5149.
    [54]
    Shang-GuanK, WangM, HtweN, et al. Lipopolysaccharides trigger two successive bursts of reactive oxygen species at distinct cellular locations[J]. Plant Physiol, 2018,176(3):2543-2556. DOI: 10.1104/pp.17.01637.
    [55]
    CaiS, ZhaoM, ZhouB, et al. Mitochondrial dysfunction in macrophages promotes inflammation and suppresses repair after myocardial infarction[J]. J Clin Invest, 2023,133(4):e159498. DOI: 10.1172/JCI159498.
  • Relative Articles

    [1]Long Luyao, Chen Yanwei, Deng Rufei, Jiang Zhenyu, Zhang Youlai. Application and research advances of delayed sural neurotrophic vascular flap for diabetic foot ulcers[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2024, 40(3): 296-300. doi: 10.3760/cma.j.cn501225-20231102-00173
    [2]Zheng Yun, Cheng Liangkun, Cui Liuchao, Tan Yuzhong, Tian Lin. Clinical effects of free dorsal interosseous artery perforator flaps in repairing multi-finger skin and soft tissue defects[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2024, 40(5): 476-481. doi: 10.3760/cma.j.cn501225-20231130-00221
    [3]Liu Xin, Huang Guangtao, Wu Jun. Research advances on the application of free flaps in limb salvage treatment of patients with diabetic foot ulcers[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2024, 40(10): 985-990. doi: 10.3760/cma.j.cn501225-20240130-00041
    [4]Zhao Jianjun, Xie Zhenjun, Zhao Guohong, Zhang Jianhua, Sun Huawei, Bai Huikai, Zhang Huifeng, Zhang Dongbin, Xiao Erhui, Zhu Guosong. Clinical effects of free anterolateral thigh perforator flaps in repairing diabetic foot ulcers under a multi-disciplinary team cooperation model[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2024, 40(8): 756-761. doi: 10.3760/cma.j.cn501225-20231107-00184
    [5]Zhang Hairui, Zhang Dongliang, Yan Xiaohui, Zhang Xiaopeng, Shang Xuliang, Meng Yanbin. Clinical effect of free posterior interosseous artery perforator flap carrying superficial vein for reconstructing severe perioral scar hyperplasia and contracture[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2023, 39(12): 1175-1179. doi: 10.3760/cma.j.cn501225-20231031-00162
    [6]Li Hai, Xiao Shun'e, Deng Chengliang, Wu Bihua, Wu Xiangkui, Zhang Tianhua, Liu Zhiyuan, Wei Zairong. Clinical application of combination of different types of free perforator flaps in the repair of complex wounds in extremities[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2023, 39(8): 758-764. doi: 10.3760/cma.j.cn501225-20220720-00300
    [7]Yang Chengpeng, Tang Linfeng, Liu Zhijin, Liu Shengzhe, Yang Lin, Cheng Junnan, Zhang Tao, Sun Fengwen, Huang Yongtao, Gao Qinfeng, Ju Jihui. Clinical effects of anterolateral thigh flap with blood supply source of medial femoral perforator in repairing the wounds on extremities[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2023, 39(9): 842-848. doi: 10.3760/cma.j.cn501225-20220726-00310
    [8]Guo Qingjiao, Ouyang Jing, Rao Jiaqin, Zhang Yizhi, Yu Lihong, Xu Wanying, Long Jinhua, Gao Xiuhua, Wu Xiaoyan, Gu Ying. Construction and preliminary validation of a risk prediction model for the recurrence of diabetic foot ulcer in diabetic patients[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2023, 39(12): 1149-1157. doi: 10.3760/cma.j.cn501225-20231101-00166
    [9]Wang Qian, Zhu Hongjuan, Feng Ying, Chu Wanli, Song Yaoyao. A cross-sectional survey and analysis of the pain status and its influencing factors in diabetic foot ulcer patients[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2023, 39(4): 330-336. doi: 10.3760/cma.j.cn501225-20220421-00150
    [10]Cao Tao, Ji Peng, Zhang Zhi, Xiao Dan, Wang Kejia, Li Na, Li Wen, Jin Guangjun, Hao Tong, Tao Ke. A prospective randomized controlled study of antibiotic bone cement in the treatment of diabetic foot ulcer[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2023, 39(4): 311-318. doi: 10.3760/cma.j.cn501225-20221111-00485
    [11]Zhang Tao, Cheng Junnan, Yang Lin, Huang Yongtao, Gao Qinfeng, Sun Fengwen, Liu Zhijin, Liu Shengzhe, Yang Chengpeng, Cao Yang, Ju Jihui. Curative effects of the superficial peroneal artery perforator flap carrying multiple perforators in repairing hand and foot wounds[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2023, 39(3): 234-240. doi: 10.3760/cma.j.cn501225-20220723-00305
    [12]Jian Yang, Wei Zairong, Chen Wei, Zhang Yanji, Tang Mingyuan, Zhong Yunxue, Liu Chenxiaoxiao. Research advances on the application of free flaps in repairing diabetic foot ulcers[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2023, 39(4): 376-380. doi: 10.3760/cma.j.cn501225-20221216-00539
    [13]Wang Peng, Chen Zhaohong, Jiang Liyuan, Zhou Xiaoqian, Jia Chiyu, Xiao Hou'an. Screening, functional analysis and clinical validation of differentially expressed genes in diabetic foot ulcers[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2022, 38(10): 944-951. doi: 10.3760/cma.j.cn501225-20220731-00328
    [14]Research progress of induced pluripotent stem cells in promoting wound healing of diabetic foot ulcers[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2022, 38(9): 864-869. doi: 10.3760/cma.j.cn501120-20210630-00230
    [15]Yu Xiaoyuan, Zhao Mingyu, Zhang Ying, Xu Gang. Research advances on the treatment of diabetic foot ulcers with autologous platelet-rich fibrin[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2022, 38(12): 1185-1189. doi: 10.3760/cma.j.cn501225-20220110-00001
    [16]Shao Jiaming, Wang Xingang, Yu Chaoheng, Han Chunmao. Teicoplanin-induced hypersensitivity syndrome in a diabetic foot patient with malignant ulcer[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2020, 36(8): 747-750. doi: 10.3760/cma.j.cn501120-20190617-00273
    [17]Shen Jinfu, Jiang Ruimei, Wang Zhuoqun, Li Mao, Li Juan, Xie Shuyong, Kang Jingjing. Recurrence and influencing factors of diabetic foot ulcer in patients with type 2 diabetes mellitus[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2020, 36(10): 947-952. doi: 10.3760/cma.j.cn501120-20190726-00315
    [18]Huang Hongjun, Niu Xihua, Yang Guanlong, Wang Liying, Shi Fanchao, Xu Shaojun, Xu Lingang, Li Yonglin. Clinical effects of application of antibiotic bone cement in wounds of diabetic foot ulcers[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2019, 35(6): 464-466. doi: 10.3760/cma.j.issn.1009-2587.2019.06.013
    [19]Gao Ya, Cui Zhengjun, Shi Xun, Guo Pengfei, Meng Qingnan, Yang Gaoyuan, Yang Rongqiang. Application of percutaneous transluminal angioplasty in the surgical treatment of patients with diabetic feet[J]. CHINESE JOURNAL OF BURNS AND WOUNDS, 2016, 32(8): 479-483. doi: 10.3760/cma.j.issn.1009-2587.2016.08.008
  • Created with Highcharts 5.0.7Amount of accessChart context menuAbstract Views, HTML Views, PDF Downloads StatisticsAbstract ViewsHTML ViewsPDF Downloads2024-042024-052024-062024-072024-082024-092024-102024-112024-122025-012025-022025-0305101520

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(6)  / Tables(2)

    Article Metrics

    Article views (681) PDF downloads(11) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return